PMID- 35802277
OWN - NLM
STAT- MEDLINE
DCOM- 20220913
LR  - 20231213
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 49
IP  - 9
DP  - 2022 Sep
TI  - LncRNA MEG3 induces endothelial differentiation of mouse derived adipose-derived 
      stem cells by targeting MiR-145-5p/KLF4.
PG  - 8495-8505
LID - 10.1007/s11033-022-07671-z [doi]
AB  - BACKGROUND: The present study aimed to investigate the mechanisms through which 
      long non-coding RNA (lncRNA) maternally expressed 3 (MEG3) affected the 
      endothelial differentiation of mouse derived adipose-derived stem cells (ADSCs). 
      MATERIALS AND METHODS: ADSCs were isolated and identified by specific surface 
      marker detection. The effects of lncRNA MEG3 on endothelial differentiation of 
      ADSCs were also detected via quantitative PCR, western blotting, 
      immunofluorescence and Matrigel angiogenesis assays. In addition, using target 
      gene prediction tools and luciferase reporter assays, the downstream target gene 
      was demonstrated. RESULTS: LncRNA MEG3 targeted and reduced the expression levels 
      of microRNA-145-5p (miR-145-5p), which upregulated the expression levels of 
      Kruppel like factor 4 (KLF4), promoting endothelial differentiation of ADSCs. 
      CONCLUSION: LncRNA MEG3 induced endothelial differentiation of ADSCs by targeting 
      miR-145-5p/KLF4, which may provide novel insights to illustrate the mechanism of 
      endothelial differentiation of ADSCs.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Zhang, Hailong
AU  - Zhang H
AD  - Department of Dermatology, First Affiliated Hospital of Heilongjiang University 
      of Traditional Chinese Medicine, Harbin, 150006, Heilongjiang, People's Republic 
      of China.
FAU - Liu, Gang
AU  - Liu G
AD  - Department of Medicine, Second Affiliated Hospital of Heilongjiang University of 
      Traditional Chinese Medicine, Harbin, 150006, Heilongjiang, People's Republic of 
      China.
FAU - Mao, Xu
AU  - Mao X
AD  - Department of Health Center, Heilongjiang University of Traditional Chinese 
      Medicine, Harbin, 150040, Heilongjiang, People's Republic of China.
FAU - Yang, Lei
AU  - Yang L
AD  - Department of Medicine, Heilongjiang Academy of Traditional Chinese Medicine, No. 
      33 of West Dazhi Street, Harbin, 150001, Heilongjiang, People's Republic of 
      China.
FAU - Wang, Bingyu
AU  - Wang B
AD  - Department of Medicine, Heilongjiang Academy of Traditional Chinese Medicine, No. 
      33 of West Dazhi Street, Harbin, 150001, Heilongjiang, People's Republic of 
      China. 243138070@qq.com.
FAU - Yuan, Xingxing
AU  - Yuan X
AUID- ORCID: 0000-0002-9894-4127
AD  - Department of Medicine, Heilongjiang Academy of Traditional Chinese Medicine, No. 
      33 of West Dazhi Street, Harbin, 150001, Heilongjiang, People's Republic of 
      China. jackie198711@163.com.
LA  - eng
GR  - ZHY2020-041/Traditional Chinese Medicine Research Project of Heilongjiang/
GR  - ZHY19-062/Traditional Chinese Medicine Research Project of Heilongjiang/
PT  - Journal Article
DEP - 20220708
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Klf4 protein, mouse)
RN  - 0 (Kruppel-Like Factor 4)
RN  - 0 (MEG3 non-coding RNA, mouse)
RN  - 0 (MIRN145a microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Animals
MH  - Cell Differentiation/genetics
MH  - *Endothelium/cytology
MH  - *Kruppel-Like Factor 4/genetics
MH  - Mice
MH  - *MicroRNAs/genetics
MH  - *RNA, Long Noncoding/genetics
MH  - *Stem Cells/metabolism
OTO - NOTNLM
OT  - Adipose-derived stem cells
OT  - Endothelial differentiation
OT  - Kruppel like factor 4
OT  - Long non-coding RNA maternally expressed 3
OT  - MicroRNA-145-5p
EDAT- 2022/07/09 06:00
MHDA- 2022/09/14 06:00
CRDT- 2022/07/08 11:18
PHST- 2022/01/19 00:00 [received]
PHST- 2022/06/01 00:00 [accepted]
PHST- 2022/07/09 06:00 [pubmed]
PHST- 2022/09/14 06:00 [medline]
PHST- 2022/07/08 11:18 [entrez]
AID - 10.1007/s11033-022-07671-z [pii]
AID - 10.1007/s11033-022-07671-z [doi]
PST - ppublish
SO  - Mol Biol Rep. 2022 Sep;49(9):8495-8505. doi: 10.1007/s11033-022-07671-z. Epub 
      2022 Jul 8.