PMID- 35802715
OWN - NLM
STAT- MEDLINE
DCOM- 20220712
LR  - 20220722
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 17
IP  - 7
DP  - 2022
TI  - Calcitriol decreases HIV-1 transfer in vitro from monocyte-derived dendritic 
      cells to CD4 + T cells, and downregulates the expression of DC-SIGN and SIGLEC-1.
PG  - e0269932
LID - 10.1371/journal.pone.0269932 [doi]
LID - e0269932
AB  - Dendritic cells (DCs) promote HIV-1 transmission by acting as Trojan horses, 
      capturing viral particles, facilitating the infection of CD4+ T-cells. Vitamin D 
      (VitD) has shown to decrease T cell activation, reducing susceptibility to HIV-1 
      infection of CD4+ T-cells in vitro; however, if VitD decreases viral transfer 
      from DCs to CD4+ T-cells is unknown. In this study, we co-cultured HIV-1-pulsed 
      immature and LPS mature monocytes-derived DCs (iDCs and LmDCs, respectively), 
      differentiated in presence or absence of calcitriol (VitD active form), with 
      PHA-activated autologous CD4+ T-cells from 16 healthy donors. In co-cultures of 
      iDCs and LmDCs treated with calcitriol, there was a significant decrease in 
      frequency of infected CD4+ T-cells, evaluated by flow cytometry. However, p24 
      levels evaluated by ELISA were not significantly reduced in culture supernatants. 
      Moreover, calcitriol-treated iDCs exhibited decreased expression of genes 
      involved in HIV-1 transfer compared to the control. Both, calcitriol-treated iDCs 
      and LmDCs exhibit a similar gene expression profile, probably related to a 
      transcriptional balance achieved after long treatment with calcitriol. Since 
      calcitriol-differentiated DCs express on their surface a lower amount of DC-SIGN 
      and SIGLEC-1 molecules, widely associated with HIV-1 transfer, suggesting that 
      this mechanism contributes to a lower transfer of viral particles by the DCs.
FAU - Alvarez, Natalia
AU  - Alvarez N
AD  - Facultad de Medicina, Grupo Inmunovirologia, Universidad de Antioquia UdeA, 
      Medellin, Colombia.
FAU - Gonzalez, Sandra M
AU  - Gonzalez SM
AD  - Facultad de Medicina, Grupo Inmunovirologia, Universidad de Antioquia UdeA, 
      Medellin, Colombia.
AD  - Department of Medical Microbiology and Infectious Diseases, University of 
      Manitoba, Winnipeg, MB, Canada.
FAU - Hernandez, Juan C
AU  - Hernandez JC
AUID- ORCID: 0000-0002-9200-5698
AD  - Facultad de Medicina, Infettare, Universidad Cooperativa de Colombia, Medellin, 
      Colombia.
FAU - Rugeles, Maria T
AU  - Rugeles MT
AD  - Facultad de Medicina, Grupo Inmunovirologia, Universidad de Antioquia UdeA, 
      Medellin, Colombia.
FAU - Aguilar-Jimenez, Wbeimar
AU  - Aguilar-Jimenez W
AD  - Facultad de Medicina, Grupo Inmunovirologia, Universidad de Antioquia UdeA, 
      Medellin, Colombia.
LA  - eng
SI  - figshare/10.6084/m9.figshare.20070209.v1
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220708
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - CD4-Positive T-Lymphocytes
MH  - Calcitriol/pharmacology
MH  - Cell Adhesion Molecules
MH  - Cells, Cultured
MH  - Dendritic Cells
MH  - *HIV Infections/prevention & control
MH  - *HIV Seropositivity
MH  - *HIV-1/physiology
MH  - Humans
MH  - Lectins, C-Type
MH  - Monocytes
MH  - Receptors, Cell Surface
MH  - Sialic Acid Binding Ig-like Lectin 1
PMC - PMC9269915
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest. The funders had no role in the design of the study; in the 
      collection, analyses, or interpretation of data; in the writing of the 
      manuscript, or in the decision to publish the results. This does not alter our 
      adherence to PLOS ONE policies on sharing data and materials.
EDAT- 2022/07/09 06:00
MHDA- 2022/07/14 06:00
PMCR- 2022/07/08
CRDT- 2022/07/08 14:14
PHST- 2021/09/09 00:00 [received]
PHST- 2022/05/31 00:00 [accepted]
PHST- 2022/07/08 14:14 [entrez]
PHST- 2022/07/09 06:00 [pubmed]
PHST- 2022/07/14 06:00 [medline]
PHST- 2022/07/08 00:00 [pmc-release]
AID - PONE-D-21-29271 [pii]
AID - 10.1371/journal.pone.0269932 [doi]
PST - epublish
SO  - PLoS One. 2022 Jul 8;17(7):e0269932. doi: 10.1371/journal.pone.0269932. 
      eCollection 2022.