PMID- 35805985
OWN - NLM
STAT- MEDLINE
DCOM- 20220712
LR  - 20220716
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 13
DP  - 2022 Jun 23
TI  - Molecular Allergen-Specific IgE Recognition Profiles and Cumulative Specific IgE 
      Levels Associated with Phenotypes of Cat Allergy.
LID - 10.3390/ijms23136984 [doi]
LID - 6984
AB  - Cat allergy is a major trigger factor for respiratory reactions (asthma and 
      rhinitis) in patients with immunoglobulin E (IgE) sensitization. In this study, 
      we used a comprehensive panel of purified cat allergen molecules (rFel d 1, nFel 
      d 2, rFel d 3, rFel d 4, rFel d 7, and rFel d 8) that were obtained by 
      recombinant expression in Escherichia coli or by purification as natural proteins 
      to study possible associations with different phenotypes of cat allergy (i.e., 
      rhinitis, conjunctivitis, asthma, and dermatitis) by analyzing molecular IgE 
      recognition profiles in a representative cohort of clinically well-characterized 
      adult cat allergic subjects (n = 84). IgE levels specific to each of the allergen 
      molecules and to natural cat allergen extract were quantified by ImmunoCAP 
      measurements. Cumulative IgE levels specific to the cat allergen molecules 
      correlated significantly with IgE levels specific to the cat allergen extract, 
      indicating that the panel of allergen molecules resembled IgE epitopes of the 
      natural allergen source. rFel d 1 represented the major cat allergen, which was 
      recognized by 97.2% of cat allergic patients; however, rFel d 3, rFel d 4, and 
      rFel d 7 each showed IgE reactivity in more than 50% of cat allergic patients, 
      indicating the importance of additional allergens in cat allergy. Patients with 
      cat-related skin symptoms showed a trend toward higher IgE levels and/or 
      frequencies of sensitization to each of the tested allergen molecules compared 
      with patients suffering only from rhinitis or asthma, while there were no such 
      differences between patients with rhinitis and asthma. The IgE levels specific to 
      allergen molecules, the IgE levels specific to cat allergen extract, and the IgE 
      levels specific to rFel d 1 were significantly higher in patients with four 
      different symptoms compared with patients with 1-2 symptoms. This difference was 
      more pronounced for the sum of IgE levels specific to the allergen molecules and 
      to cat extract than for IgE levels specific for rFel d 1 alone. Our study 
      indicates that, in addition to rFel d 1, rFel d 3, rFel d 4, and rFel d 7 must be 
      considered as important cat allergens. Furthermore, the cumulative sum of IgE 
      levels specific to cat allergen molecules seems to be a biomarker for identifying 
      patients with complex phenotypes of cat allergy. These findings are important for 
      the diagnosis of IgE sensitization to cats and for the design of 
      allergen-specific immunotherapies for the treatment and prevention of cat 
      allergy.
FAU - Riabova, Ksenja
AU  - Riabova K
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergy, 
      Sechenov First Moscow State Medical University, 119991 Moscow, Russia.
FAU - Karsonova, Antonina V
AU  - Karsonova AV
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergy, 
      Sechenov First Moscow State Medical University, 119991 Moscow, Russia.
FAU - van Hage, Marianne
AU  - van Hage M
AUID- ORCID: 0000-0003-3091-1596
AD  - Division of Immunology and Allergy, Department of Medicine Solna, Karolinska 
      Institutet and Karolinska University Hospital, SE-171 77 Stockholm, Sweden.
FAU - Kack, Ulrika
AU  - Kack U
AD  - Department of Clinical Science and Education, Sodersjukhuset, Karolinska 
      Institutet, SE-171 77 Stockholm, Sweden.
FAU - Konradsen, Jon R
AU  - Konradsen JR
AUID- ORCID: 0000-0001-7745-8624
AD  - Astrid Lindgren Children's Hospital, Karolinska University Hospital, SE-171 64 
      Stockholm, Sweden.
AD  - Department of Women's and Children's Health, Karolinska Institutet, SE-171 77 
      Stockholm, Sweden.
FAU - Gronlund, Hans
AU  - Gronlund H
AUID- ORCID: 0000-0003-4882-7624
AD  - Therapeutic Immune Design Unit, Department of Clinical Neuroscience, Karolinska 
      Institutet, SE-171 77 Stockholm, Sweden.
FAU - Fomina, Daria
AU  - Fomina D
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergy, 
      Sechenov First Moscow State Medical University, 119991 Moscow, Russia.
AD  - Moscow City Center of Allergy and Immunology, Clinical City Hospital #52, 123182 
      Moscow, Russia.
FAU - Beltyukov, Evgeny
AU  - Beltyukov E
AD  - Ural State Medical University, 620014 Ekaterinburg, Russia.
FAU - Glazkova, Polina A
AU  - Glazkova PA
AUID- ORCID: 0000-0002-8830-7503
AD  - Laboratory of Medical and Physics Research, Moscow Regional Research and Clinical 
      Institute ("MONIKI"), 129110 Moscow, Russia.
FAU - Semenov, Dmitry Yu
AU  - Semenov DY
AD  - Thoracic Surgery Center, Saint-Petersburg State Research Institute of 
      Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation, 
      194291 Saint-Petersburg, Russia.
FAU - Valenta, Rudolf
AU  - Valenta R
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergy, 
      Sechenov First Moscow State Medical University, 119991 Moscow, Russia.
AD  - Karl Landsteiner University for Healthcare Sciences, 3500 Krems, Austria.
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Karaulov, Alexander
AU  - Karaulov A
AUID- ORCID: 0000-0002-1930-5424
AD  - Laboratory of Immunopathology, Department of Clinical Immunology and Allergy, 
      Sechenov First Moscow State Medical University, 119991 Moscow, Russia.
FAU - Curin, Mirela
AU  - Curin M
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, 1090 Vienna, Austria.
LA  - eng
GR  - na/DARC Program of the Country of Lower Austria/
PT  - Journal Article
DEP - 20220623
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Allergens)
RN  - 0 (Glycoproteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens
MH  - *Alveolitis, Extrinsic Allergic
MH  - *Asthma
MH  - Glycoproteins/genetics
MH  - Humans
MH  - *Hypersensitivity/diagnosis
MH  - Immunoglobulin E/genetics
MH  - Phenotype
MH  - *Rhinitis
PMC - PMC9266786
OTO - NOTNLM
OT  - IgE reactivity
OT  - allergen molecules
OT  - allergy
OT  - allergy diagnosis
OT  - allergy phenotypes
OT  - cat allergens
OT  - cat allergy
COIS- Rudolf Valenta has received research grants from WORG Pharmaceutical (Hangzhou, 
      China), HVD Biotech (Vienna, Austria), and Viravaxx AG (Vienna, Austria). He 
      serves as a consultant for WORG and Viravaxx. The other authors declare no 
      conflicts of interest. The funders had no role in the design of the study; in the 
      collection, analyses, or interpretation of data; in the writing of the 
      manuscript; or in the decision to publish the results.
EDAT- 2022/07/10 06:00
MHDA- 2022/07/14 06:00
PMCR- 2022/06/23
CRDT- 2022/07/09 01:10
PHST- 2022/05/06 00:00 [received]
PHST- 2022/06/13 00:00 [revised]
PHST- 2022/06/17 00:00 [accepted]
PHST- 2022/07/09 01:10 [entrez]
PHST- 2022/07/10 06:00 [pubmed]
PHST- 2022/07/14 06:00 [medline]
PHST- 2022/06/23 00:00 [pmc-release]
AID - ijms23136984 [pii]
AID - ijms-23-06984 [pii]
AID - 10.3390/ijms23136984 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Jun 23;23(13):6984. doi: 10.3390/ijms23136984.