PMID- 35806463
OWN - NLM
STAT- MEDLINE
DCOM- 20220712
LR  - 20230308
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 13
DP  - 2022 Jul 5
TI  - Investigation of the Molecular Mechanisms Underlying the Antiatherogenic Actions 
      of Kaempferol in Human THP-1 Macrophages.
LID - 10.3390/ijms23137461 [doi]
LID - 7461
AB  - Cardiovascular disease (CVD) is causing high mortality worldwide (World Health 
      Organization-WHO, 2015). Atherosclerosis, the hardening and narrowing of arteries 
      caused by the accumulation of fatty acids and lipids (cholesterol plaques), is a 
      main reason of stroke, myocardial infarction, and angina. Present therapies for 
      cardiovascular disease basically use statins such as beta-Hydroxy 
      beta-methylglutaryl-CoA, with <70% efficacy and multiple side effects. An in vitro 
      investigation was conducted to evaluate the impact of kaempferol, a natural 
      medication, in an atherosclerotic cell model. We used cytotoxicity assays, Boyden 
      chamber invasion assays, and quantitative PCR. Affymetrix microarrays were used 
      to profile the entire transcriptome of kaempferol-treated cell lines, and Partek 
      Genomic Suite was used to interpret the results. Kaempferol was not cytotoxic to 
      THP-1 macrophages. In comparison to the control, kaempferol reduced monocyte 
      migration mediated by monocyte chemotactic protein 1 (MCP-1) by 80%. The qPCR 
      results showed a 73.7-fold reduction in MCP-1 and a 2.5-fold reduction in 
      intercellular adhesion molecule 1 (ICAM-1) expression in kaempferol-treated 
      cells. In interferon gamma (IFN-gamma) without kaempferol and IFN-gamma with kaempferol 
      treated cells, we found 295 and 168 differentially expressed genes (DEGs), 
      respectively. According to DEG pathway analysis, kaempferol exhibits 
      anti-atherosclerosis and anti-inflammatory characteristics. Kaempferol is an 
      effective and safe therapy for atherosclerosis.
FAU - Huwait, Etimad
AU  - Huwait E
AUID- ORCID: 0000-0002-4657-6668
AD  - Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah 
      21589, Saudi Arabia.
AD  - Cell Culture Unit and Experimental Biochemistry Unit, King Fahd Medical Research 
      Centre, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
FAU - Ayoub, Maha
AU  - Ayoub M
AUID- ORCID: 0000-0002-1491-7851
AD  - Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah 
      21589, Saudi Arabia.
AD  - Cell Culture Unit and Experimental Biochemistry Unit, King Fahd Medical Research 
      Centre, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
FAU - Karim, Sajjad
AU  - Karim S
AUID- ORCID: 0000-0003-0907-2097
AD  - Center of Excellence in Genomic Medicine Research, King Abdulaziz University, 
      Jeddah 21589, Saudi Arabia.
AD  - Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, 
      King Abdulaziz University, Jeddah 21589, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20220705
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Chemokine CCL2)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - *Atherosclerosis/drug therapy/metabolism
MH  - *Cardiovascular Diseases/metabolism
MH  - Chemokine CCL2/genetics/metabolism
MH  - Cholesterol/metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Macrophages/metabolism
MH  - Monocytes/metabolism
PMC - PMC9267302
OTO - NOTNLM
OT  - Affymetrix microarrays
OT  - ICAM-1
OT  - IFN-gamma
OT  - MCP-1
OT  - cardiovascular disease atherosclerosis
OT  - human THP-1 macrophages
OT  - kaempferol
COIS- The authors declare that they have no competing interests.
EDAT- 2022/07/10 06:00
MHDA- 2022/07/14 06:00
PMCR- 2022/07/05
CRDT- 2022/07/09 01:13
PHST- 2022/06/14 00:00 [received]
PHST- 2022/06/29 00:00 [revised]
PHST- 2022/06/30 00:00 [accepted]
PHST- 2022/07/09 01:13 [entrez]
PHST- 2022/07/10 06:00 [pubmed]
PHST- 2022/07/14 06:00 [medline]
PHST- 2022/07/05 00:00 [pmc-release]
AID - ijms23137461 [pii]
AID - ijms-23-07461 [pii]
AID - 10.3390/ijms23137461 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Jul 5;23(13):7461. doi: 10.3390/ijms23137461.