PMID- 35807667
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2223-7747 (Print)
IS  - 2223-7747 (Electronic)
IS  - 2223-7747 (Linking)
VI  - 11
IP  - 13
DP  - 2022 Jun 28
TI  - Dual Beneficial Effects of Methylnissolin-3-O-beta-d-Glucopyranoside on 
      Obesity-Induced Inflammatory Responses in Adipocyte-Macrophage Co-Culture.
LID - 10.3390/plants11131715 [doi]
LID - 1715
AB  - Methylnissolin-3-O-beta-d-glucopyranoside (MNG) is a pterocarpan analog, which 
      protects EA.hy926 cells against oxidative damage through the Nrf2/HO-1 pathway. 
      However, the effects of MNG on obesity-induced inflammatory responses in 
      adipocyte-macrophage co-culture remain unclear. A differentiated murine 
      preadipocyte cell line (3T3-L1) was co-cultured with a murine macrophage cell 
      line (RAW264.7). Intracellular lipid accumulation was determined using Oil Red O 
      staining. Western blotting was performed to investigate the expression of 
      adipogenesis- and inflammation-associated proteins. Cell culture supernatants 
      were assayed using ELISA kits to measure the levels of proinflammatory cytokines 
      such as interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1). MNG 
      inhibited lipid accumulation and the production of IL-6 and MCP-1 in the 3T3-L1 
      and RAW264.7 cell co-culture. Moreover, MNG inhibited the protein expression of 
      CCAAT/enhancer-binding protein alpha (C/EBPalpha), C/EBPbeta, peroxisome 
      proliferator-activated receptor gamma (PPARgamma), cyclooxygenase 2 (COX-2), and 
      inducible nitric oxide synthase (iNOS) under the same co-culture conditions. MNG 
      also inhibited IL-6 and MCP-1 production compared with the co-culture control. 
      These findings demonstrate that MNG inhibited lipid accumulation and inflammatory 
      response by downregulating IL-6 and MCP-1 production and protein expression of 
      C/EBPbeta, C/EBPalpha, PPARgamma, COX-2, and iNOS in co-culture conditions with 3T3-L1 and 
      RAW264.7 cells. These results suggest that MNG may be beneficial in preventing 
      obesity-related inflammatory status.
FAU - Lee, Dahae
AU  - Lee D
AD  - College of Korean Medicine, Gachon University, Seongnam 13120, Korea.
FAU - Wu, Xiaohua
AU  - Wu X
AD  - Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, 
      University of Hawai'i at Hilo, Hilo, HI 96720, USA.
FAU - Lange, Ingo
AU  - Lange I
AD  - Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, 
      University of Hawai'i at Hilo, Hilo, HI 96720, USA.
FAU - Cao, Shugeng
AU  - Cao S
AUID- ORCID: 0000-0001-6684-8221
AD  - Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, 
      University of Hawai'i at Hilo, Hilo, HI 96720, USA.
FAU - Kang, Ki Sung
AU  - Kang KS
AD  - College of Korean Medicine, Gachon University, Seongnam 13120, Korea.
LA  - eng
GR  - 2020M3A9E4104380/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20220628
PL  - Switzerland
TA  - Plants (Basel)
JT  - Plants (Basel, Switzerland)
JID - 101596181
PMC - PMC9269391
OTO - NOTNLM
OT  - adipocytes
OT  - adipogenesis
OT  - inflammation
OT  - macrophage
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/10 06:00
MHDA- 2022/07/10 06:01
PMCR- 2022/06/28
CRDT- 2022/07/09 01:20
PHST- 2022/06/13 00:00 [received]
PHST- 2022/06/22 00:00 [revised]
PHST- 2022/06/24 00:00 [accepted]
PHST- 2022/07/09 01:20 [entrez]
PHST- 2022/07/10 06:00 [pubmed]
PHST- 2022/07/10 06:01 [medline]
PHST- 2022/06/28 00:00 [pmc-release]
AID - plants11131715 [pii]
AID - plants-11-01715 [pii]
AID - 10.3390/plants11131715 [doi]
PST - epublish
SO  - Plants (Basel). 2022 Jun 28;11(13):1715. doi: 10.3390/plants11131715.