PMID- 35809284
OWN - NLM
STAT- MEDLINE
DCOM- 20220712
LR  - 20220712
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 67
IP  - 4
DP  - 2022 Jan 2
TI  - Diagnosis and detection of VicK gene in Streptococcus mutans isolated from the 
      saliva of patients with diabetic type 2 with tooth decay in the Iraqi population.
PG  - 222-231
LID - 10.14715/cmb/2021.67.4.25 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is gradually becoming more common in Iraq. 
      Salivary changes and proliferation of specific bacterial communities cause oral 
      disease that can adversely affect systemic conditions such as diabetes. Fifty 
      saliva samples were collected from people with T2DM suffering from tooth decay 
      and twenty-five people without T2DM suffering from tooth decay. The periodontal 
      status, the extent of the root surface, and coronal caries were evaluated. Saliva 
      was cultured for investigating Streptococcus mutans. The results showed that 
      patients with type 2 diabetes had significantly more severe Periodontitis and a 
      higher prevalence and magnitude of bacterial caries. Diabetic subjects had higher 
      levels of Hemoglobin A1c (HbA1c) and Random Blood Sugar (R.B.S.). The S. mutans 
      diagnosis by PCR for Sanger Sequencing technique by using VicK gene sequences 
      (1300bp). The PCR products of the isolate were submitted to Macrogen Company for 
      sequencing. Selected seven isolates as new isolates registered in global gene 
      bank as locally S. Mutans isolates in Bagdad city/Iraq and their accepted 
      accession numbers include LOCUS MT603520, MT603521, MT603522, MT603523, MT603524, 
      MT603525,and MT603526 of nucleotide sequence. The VicK genes isolates' 
      phylogenetic trees revealed a genotype that was closely connected to other 
      isolates in GenBank. Furthermore, gene sequencing demonstrated a success rate of 
      99 percent. resemblance to other isolates in the GenBank database The likelihood 
      of a link between S. Mutans and dental carries was determined by these findings.
FAU - Al-Sudani, Susan F Khadhem
AU  - Al-Sudani SFK
AD  - Lecture in Medical Microbiology, Pathology & Genetic, College of Dentistry, 
      Al-Bayan University, Baghdad, Iraq. Fattima.ali@hmu.edu.krd.
FAU - Hamad, Laheeb R
AU  - Hamad LR
AD  - Lecturer in Microbiology, Department of Pathological Analysis, College of Applied 
      Sciences, Al Fallujah University, Iraq. Fattima.ali@hmu.edu.krd.
FAU - Ali, Fattma A
AU  - Ali FA
AD  - Assistant Professor in Medical Microbiology, Medical Microbiology Department, 
      College of Health Sciences, Hawler Medical University, Kurdistan Region-Iraq. 
      Fattima.ali@hmu.edu.krd.
LA  - eng
PT  - Journal Article
DEP - 20220102
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
SB  - IM
MH  - *Diabetes Mellitus, Type 2/complications/genetics
MH  - Humans
MH  - Iraq
MH  - Phylogeny
MH  - Saliva/microbiology
MH  - *Streptococcus mutans/genetics
EDAT- 2022/07/10 06:00
MHDA- 2022/07/14 06:00
CRDT- 2022/07/09 17:12
PHST- 2022/01/01 00:00 [received]
PHST- 2022/07/09 17:12 [entrez]
PHST- 2022/07/10 06:00 [pubmed]
PHST- 2022/07/14 06:00 [medline]
AID - 10.14715/cmb/2021.67.4.25 [doi]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2022 Jan 2;67(4):222-231. doi: 
      10.14715/cmb/2021.67.4.25.