PMID- 35812888
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 9
DP  - 2022
TI  - Evolutionary Analysis of Four Recombinant Viruses of the Porcine Reproductive and 
      Respiratory Syndrome Virus From a Pig Farm in China.
PG  - 933896
LID - 10.3389/fvets.2022.933896 [doi]
LID - 933896
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) is one of the 
      most important pathogens causing substantial economic losses to the Chinese swine 
      industry. In this study, we analyzed the complete genome sequences of four PRRSV 
      isolates (PRRSV2/CN/SS0/2020, PRRSV2/CN/SS1/2021, PRRSV2/CN/L3/2021, and 
      PRRSV2/CN/L4/2020) isolated from a single pig farm from 2020 to 2021. The genomes 
      of the four isolates were 14,962-15,023 nt long, excluding the poly (A) tails. 
      Comparative analysis of the genome sequences showed that the four isolates shared 
      93.2-98.1% homology and they had no close PRRSV relatives registered in the 
      GenBank (<92%). Furthermore, PRRSV2/CN/SS0/2020 and PRRSV2/CN/SS1/2021 had 
      characteristic 150-aa deletions (aa481+aa537-566 +aa628-747) that were identical 
      to the live attenuated virus vaccine strain TJM-F92 (derived from the HP-PRRSV 
      TJ). Further analysis of the full-length sequences suggests that the four 
      isolates were natural recombinant strains between lineages 1 (NADC30-like), 3 
      (QYYZ-like), and 8.7 (JXA1-like). Animal experiments revealed discrepancies in 
      virulence between PRRSV2/CN/SS0/2020 and PRRSV2/CN/L3/2021. The strain with high 
      homology to HP-PRRSV demonstrates higher pathogenicity for pigs than the other 
      isolate with low homology to HP-PRRSV. Taken together, our findings suggest that 
      PRRSVs have undergone genome evolution by recombination among field 
      strains/MLV-like strains of different lineages.
CI  - Copyright (c) 2022 Liu, Lai, Xu, Yang, Li, Liu, Hunag and Wei.
FAU - Liu, Jiankui
AU  - Liu J
AD  - College of Life Sciences, Longyan University, Longyan, China.
AD  - Engineering Research Center for the Prevention and Control of Animal Original 
      Zoonosis, Fujian Province University, College of Life Science, Longyan 
      University, Longyan, China.
AD  - College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 
      China.
FAU - Lai, Liling
AU  - Lai L
AD  - College of Life Sciences, Longyan University, Longyan, China.
FAU - Xu, Ye
AU  - Xu Y
AD  - College of Life Sciences, Longyan University, Longyan, China.
AD  - College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 
      China.
FAU - Yang, Yuan
AU  - Yang Y
AD  - College of Life Sciences, Longyan University, Longyan, China.
FAU - Li, Jiarui
AU  - Li J
AD  - College of Life Sciences, Longyan University, Longyan, China.
FAU - Liu, Chen
AU  - Liu C
AD  - College of Life Sciences, Longyan University, Longyan, China.
AD  - College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 
      China.
FAU - Hunag, Cuiqin
AU  - Hunag C
AD  - College of Life Sciences, Longyan University, Longyan, China.
AD  - Engineering Research Center for the Prevention and Control of Animal Original 
      Zoonosis, Fujian Province University, College of Life Science, Longyan 
      University, Longyan, China.
AD  - College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 
      China.
FAU - Wei, Chunhua
AU  - Wei C
AD  - College of Life Sciences, Longyan University, Longyan, China.
AD  - Engineering Research Center for the Prevention and Control of Animal Original 
      Zoonosis, Fujian Province University, College of Life Science, Longyan 
      University, Longyan, China.
AD  - College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220624
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC9270021
OTO - NOTNLM
OT  - evolution
OT  - genome characterization
OT  - pathogenicity
OT  - porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - recombination
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/12 06:00
MHDA- 2022/07/12 06:01
PMCR- 2022/01/01
CRDT- 2022/07/11 03:52
PHST- 2022/05/01 00:00 [received]
PHST- 2022/05/30 00:00 [accepted]
PHST- 2022/07/11 03:52 [entrez]
PHST- 2022/07/12 06:00 [pubmed]
PHST- 2022/07/12 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2022.933896 [doi]
PST - epublish
SO  - Front Vet Sci. 2022 Jun 24;9:933896. doi: 10.3389/fvets.2022.933896. eCollection 
      2022.