PMID- 35814427
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 12
DP  - 2022
TI  - High CBX8 Expression Leads to Poor Prognosis in Laryngeal Squamous Cell Carcinoma 
      by Inducing EMT by Activating the Wnt/beta-Catenin Signaling Pathway.
PG  - 881262
LID - 10.3389/fonc.2022.881262 [doi]
LID - 881262
AB  - BACKGROUND: In this study, we detected the expression of chromobox protein 
      homolog 8 (CBX8) in laryngeal squamous cell carcinoma (LSCC) and its influence on 
      the occurrence and progression of LSCC. METHODS: Pancancer analysis of CBX8 was 
      analyzed by TCGA database and its expression in LSCC.The expression of CBX8 in 30 
      pairs of LSCC and adjacent tissues was analyzed by quantitative real-time 
      PCR(qRT-PCR)and immunohistochemical assays, and its association with the 
      prognosis and clinicopathological features of LSCC was further evaluated. A CBX8 
      knockdown model was constructed in AMC-HN-8 and Hep2 cell lines. The effects of 
      CBX8 on LSCC cell proliferation, migration, invasion and apoptosis were detected 
      by CCK8,EdU,wound healing, Transwell and flow cytometry assays. Levels of 
      apoptosis-related protein, WNT/beta-catenin signaling pathway and epithelial to 
      mesenchymal transition (EMT) proteins, including Bax, Bcl2, beta-catenin, DKK1, 
      GSK3beta, N-cadherin, E-cadherin and Snail1, in LSCC cells were detected by Western 
      blotting. RESULTS: CBX8 was overexpressed in LSCC. High expression of CBX8 in 
      LSCC patients led to shorter overall survival and correlated with tumor stage and 
      lymphatic metastasis. After CBX8 knockdown, the proliferation of AMC-HN-8 and 
      Hep2 cells slowed, and the number of EdU-positive cells decreased. Wound healing 
      slowed down, and the number of Transwell invading cells decreased. The percentage 
      of apoptotic cells increased. The expression levels of Bcl2, beta-catenin, 
      N-cadherin and Snail11 proteins were significantly reduced in the CBX8 knockdown 
      cells, while Bax, DKK1, GSK3beta and E-cadherin significantly increased with 
      their corresponding controls. CONCLUSION: CBX8 is highly expressed in LSCC and 
      induces the EMT process by activating the Wnt/beta-catenin signaling pathway to 
      promote LSCC cell proliferation and migration and inhibit apoptosis, resulting in 
      poor prognosis.
CI  - Copyright (c) 2022 Meng, Li and Wang.
FAU - Meng, Qingchao
AU  - Meng Q
AD  - Department of Otolaryngology, Shengjing Hospital of China Medical University, 
      Shenyang, China.
FAU - Li, Lei
AU  - Li L
AD  - Department of Urology, ShengJing Hospital of China Medical University, Shenyang, 
      China.
FAU - Wang, Liping
AU  - Wang L
AD  - Department of Otolaryngology, Shengjing Hospital of China Medical University, 
      Shenyang, China.
LA  - eng
PT  - Journal Article
DEP - 20220623
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC9259798
OTO - NOTNLM
OT  - CBX8
OT  - EMT
OT  - LSCC
OT  - TCGA
OT  - Wnt/beta-catenin
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/12 06:00
MHDA- 2022/07/12 06:01
PMCR- 2022/01/01
CRDT- 2022/07/11 04:17
PHST- 2022/02/22 00:00 [received]
PHST- 2022/05/25 00:00 [accepted]
PHST- 2022/07/11 04:17 [entrez]
PHST- 2022/07/12 06:00 [pubmed]
PHST- 2022/07/12 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2022.881262 [doi]
PST - epublish
SO  - Front Oncol. 2022 Jun 23;12:881262. doi: 10.3389/fonc.2022.881262. eCollection 
      2022.