PMID- 35816250
OWN - NLM
STAT- MEDLINE
DCOM- 20220919
LR  - 20230201
IS  - 1439-7633 (Electronic)
IS  - 1439-4227 (Print)
IS  - 1439-4227 (Linking)
VI  - 23
IP  - 18
DP  - 2022 Sep 16
TI  - Peroxidase Activity of Myoglobin Variants Reconstituted with Artificial 
      Cofactors.
PG  - e202200197
LID - 10.1002/cbic.202200197 [doi]
LID - e202200197
AB  - Myoglobin (Mb) can react with hydrogen peroxide (H(2) O(2) ) to form a highly 
      active intermediate compound and catalyse oxidation reactions. To enhance this 
      activity, known as pseudo-peroxidase activity, previous studies have focused on 
      the modification of key amino acid residues of Mb or the heme cofactor. In this 
      work, the Mb scaffold (apo-Mb) was systematically reconstituted with a set of 
      cofactors based on six metal ions and two ligands. These Mb variants were fully 
      characterised by UV-Vis spectroscopy, circular dichroism (CD) spectroscopy, 
      inductively coupled plasma mass spectrometry (ICP-MS) and native mass 
      spectrometry (nMS). The steady-state kinetics of guaiacol oxidation and 
      2,4,6-trichlorophenol (TCP) dehalogenation catalysed by Mb variants were 
      determined. Mb variants with iron chlorin e6 (Fe-Ce6) and manganese chlorin e6 
      (Mn-Ce6) cofactors were found to have improved catalytic efficiency for both 
      guaiacol and TCP substrates in comparison with wild-type Mb, i. e. 
      Fe-protoporphyrin IX-Mb. Furthermore, the selected cofactors were incorporated 
      into the scaffold of a Mb mutant, swMb H64D. Enhanced peroxidase activity for 
      both substrates were found via the reconstitution of Fe-Ce6 into the mutant 
      scaffold.
CI  - (c) 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.
FAU - Guo, Chao
AU  - Guo C
AUID- ORCID: 0000-0002-1610-1768
AD  - Department of Pure and Applied Chemistry, University of Strathclyde, 295 
      Cathedral Street, G1 1XL, Glasgow, UK.
FAU - Chadwick, Robert J
AU  - Chadwick RJ
AUID- ORCID: 0000-0001-7476-2399
AD  - Department of Pure and Applied Chemistry, University of Strathclyde, 295 
      Cathedral Street, G1 1XL, Glasgow, UK.
FAU - Foulis, Adam
AU  - Foulis A
AD  - Department of Pure and Applied Chemistry, University of Strathclyde, 295 
      Cathedral Street, G1 1XL, Glasgow, UK.
FAU - Bedendi, Giada
AU  - Bedendi G
AD  - Department of Inorganic and Analytical Chemistry, University of Geneva, 1211, 
      Geneva 4, Switzerland.
FAU - Lubskyy, Andriy
AU  - Lubskyy A
AUID- ORCID: 0000-0002-6725-4430
AD  - Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700, 
      Fribourg, Switzerland.
FAU - Rodriguez, Kyle J
AU  - Rodriguez KJ
AUID- ORCID: 0000-0002-3208-8612
AD  - Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700, 
      Fribourg, Switzerland.
FAU - Pellizzoni, Michela M
AU  - Pellizzoni MM
AUID- ORCID: 0000-0003-4294-0247
AD  - Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700, 
      Fribourg, Switzerland.
FAU - Milton, Ross D
AU  - Milton RD
AUID- ORCID: 0000-0002-2229-0243
AD  - Department of Inorganic and Analytical Chemistry, University of Geneva, 1211, 
      Geneva 4, Switzerland.
FAU - Beveridge, Rebecca
AU  - Beveridge R
AUID- ORCID: 0000-0003-0320-6496
AD  - Department of Pure and Applied Chemistry, University of Strathclyde, 295 
      Cathedral Street, G1 1XL, Glasgow, UK.
FAU - Bruns, Nico
AU  - Bruns N
AUID- ORCID: 0000-0001-6199-9995
AD  - Department of Pure and Applied Chemistry, University of Strathclyde, 295 
      Cathedral Street, G1 1XL, Glasgow, UK.
AD  - Department of Chemistry, Technical University of Darmstadt, Alarich-Weiss-Str. 4, 
      64287, Darmstadt, Germany.
LA  - eng
GR  - MR/T020970/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220728
PL  - Germany
TA  - Chembiochem
JT  - Chembiochem : a European journal of chemical biology
JID - 100937360
RN  - 0 (Amino Acids)
RN  - 0 (Myoglobin)
RN  - 42VZT0U6YR (Heme)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 6JKA7MAH9C (Guaiacol)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.- (Peroxidases)
SB  - IM
MH  - Amino Acids
MH  - Guaiacol
MH  - Heme/chemistry
MH  - *Hydrogen Peroxide/chemistry
MH  - Manganese
MH  - *Myoglobin/chemistry/genetics/metabolism
MH  - Peroxidases/metabolism
PMC - PMC9545363
OTO - NOTNLM
OT  - metalloproteins
OT  - mutagenesis
OT  - myoglobin
OT  - peroxidases
OT  - reconstitution
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/12 06:00
MHDA- 2022/09/20 06:00
PMCR- 2022/10/07
CRDT- 2022/07/11 11:15
PHST- 2022/07/08 00:00 [revised]
PHST- 2022/04/06 00:00 [received]
PHST- 2022/07/12 06:00 [pubmed]
PHST- 2022/09/20 06:00 [medline]
PHST- 2022/07/11 11:15 [entrez]
PHST- 2022/10/07 00:00 [pmc-release]
AID - CBIC202200197 [pii]
AID - 10.1002/cbic.202200197 [doi]
PST - ppublish
SO  - Chembiochem. 2022 Sep 16;23(18):e202200197. doi: 10.1002/cbic.202200197. Epub 
      2022 Jul 28.