PMID- 35816841
OWN - NLM
STAT- MEDLINE
DCOM- 20220823
LR  - 20220823
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 242
DP  - 2022 Sep 1
TI  - Isoprocarb causes neurotoxicity of zebrafish embryos through oxidative 
      stress-induced apoptosis.
PG  - 113870
LID - S0147-6513(22)00710-2 [pii]
LID - 10.1016/j.ecoenv.2022.113870 [doi]
AB  - Isoprocarb is a widely used carbamate insecticide in agriculture and aquaculture. 
      Overuse of isoprocarb always leaves toxic residues in soil and water, however, 
      the potential ecotoxicity of isoprocarb to organisms is still confusing. In this 
      study, zebrafish embryo was used as a model to evaluate the toxicity of 
      isoprocarb. Zebrafish embryos (96 hpf) were separately exposed at different 
      concentrations of isoprocarb. The mortality rate, hatchability rate, average 
      heart beat of the zebrafish embryo were separately calculated. Our results 
      suggested that exposure to isoprocarb induced developmental toxicity in zebrafish 
      embryos. HE staining showed that exposure to isoprocarb caused developmental 
      defect in the hindbrain of zebrafish embryos. As expected, the behavioral 
      analysis also showed that the motor ability of zebrafish embryos were 
      significantly inhibited following exposure to isoprocarb. In terms of mechanism, 
      The expressions of genes involved in neurodevelopment signaling pathways, such as 
      foxo3a, gfap, syn2a, elavl3 and sox19b, were inhibited in zebrafish embryos after 
      exposure to isoprocarb. The acetylcholinesterase (AChE) activity was also reduced 
      in isoprocarb-treated zebrafish embryos. Moreover, oxidative stress was induced 
      by increasing the reactive oxygen species (ROS) level and decreasing the activity 
      of antioxidant enzyme (SOD) after exposure to isoprocarb. Expectedly, acridine 
      orange (AO) staining and the detection of some apoptosis-related genes revealed 
      that oxidative stress resulted in apoptosis. In short, the expressions of genes 
      associated with the neurodevelopmental signaling pathway are inhibited, and 
      oxidative stress is also induced in zebrafish embryos after exposure to 
      isoprocarb, which may be the molecular basics of isoprocarb-induced neurotoxicity 
      in zebrafish embryos.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Wang, Shanghong
AU  - Wang S
AD  - School of Life Science, Nanchang University, Nanchang 330031, Jiangxi, China.
FAU - Han, Xue
AU  - Han X
AD  - School of Life Science, Nanchang University, Nanchang 330031, Jiangxi, China.
FAU - Yu, Tingting
AU  - Yu T
AD  - School of Life Science, Nanchang University, Nanchang 330031, Jiangxi, China.
FAU - Liu, Yulong
AU  - Liu Y
AD  - School of Life Science, Nanchang University, Nanchang 330031, Jiangxi, China.
FAU - Zhang, Hongying
AU  - Zhang H
AD  - School of Life Science, Nanchang University, Nanchang 330031, Jiangxi, China.
FAU - Mao, Huiling
AU  - Mao H
AD  - School of Life Science, Nanchang University, Nanchang 330031, Jiangxi, China.
FAU - Hu, Chengyu
AU  - Hu C
AD  - School of Life Science, Nanchang University, Nanchang 330031, Jiangxi, China.
FAU - Xu, Xiaowen
AU  - Xu X
AD  - School of Life Science, Nanchang University, Nanchang 330031, Jiangxi, China; 
      State Key Laboratory of Food Science and Technology, Nanchang University, 
      Nanchang 330047, Jiangxi, China; Jiangxi Provincial Key Laboratory of 
      Interdisciplinary Science, Nanchang University, Nanchang 330031, Jiangxi, China. 
      Electronic address: xuxw2020@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220708
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Carbamates)
RN  - 0 (SOX Transcription Factors)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (sox19b protein, zebrafish)
RN  - 714I55QH9K (isoprocarb)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
SB  - IM
MH  - Acetylcholinesterase/metabolism
MH  - Animals
MH  - Apoptosis/genetics
MH  - Carbamates/metabolism
MH  - Embryo, Nonmammalian/metabolism
MH  - *Neurotoxicity Syndromes/metabolism
MH  - Oxidative Stress
MH  - SOX Transcription Factors/metabolism
MH  - *Water Pollutants, Chemical/metabolism/toxicity
MH  - Zebrafish/metabolism
MH  - Zebrafish Proteins/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Carbamate insecticide
OT  - Neurodevelopment
OT  - Oxidative stress
OT  - Zebrafish embryos
EDAT- 2022/07/12 06:00
MHDA- 2022/08/24 06:00
CRDT- 2022/07/11 18:17
PHST- 2022/04/01 00:00 [received]
PHST- 2022/07/02 00:00 [revised]
PHST- 2022/07/07 00:00 [accepted]
PHST- 2022/07/12 06:00 [pubmed]
PHST- 2022/08/24 06:00 [medline]
PHST- 2022/07/11 18:17 [entrez]
AID - S0147-6513(22)00710-2 [pii]
AID - 10.1016/j.ecoenv.2022.113870 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2022 Sep 1;242:113870. doi: 10.1016/j.ecoenv.2022.113870. 
      Epub 2022 Jul 8.