PMID- 35817112
OWN - NLM
STAT- MEDLINE
DCOM- 20220908
LR  - 20220914
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 845
DP  - 2022 Nov 1
TI  - The effects of endosulfan on cell migration and invasion in prostate cancer cells 
      via the KCNQ1OT1/miR-137-3p/PTP4A3 axis.
PG  - 157252
LID - S0048-9697(22)04350-9 [pii]
LID - 10.1016/j.scitotenv.2022.157252 [doi]
AB  - Endosulfan belongs to persistent organic pollutants (POPs), closely related to an 
      increased risk of prostate cancer (PCa). The existing evidence shows that lncRNAs 
      compete with miRNAs for binding sites and contribute to the onset and progression 
      of human malignancies. In this study we investigate how endosulfan promotes cell 
      migration and invasion in DU145 and PC3 prostate cancer cells through epigenetic 
      mechanism of lncRNA-miRNA regulation. Based on our past research we focused on 
      PTP4A3 and constructed wild-type (WT) and mutant PTP4A3 plasmids for further 
      analysis. Our results revealed that transfection of PTP4A3-WT can lead to changes 
      in the expression of epithelial-mesenchymal transition (EMT) biomarkers and 
      critical proteins in the TGF-beta signaling pathway, and promote cell migration and 
      invasion in PCa cells. Bioinformatics analysis shows that there were 
      complementary sequences in PTP4A3 3'-UTR and KCNQ1OT1 3'-UTR to the seed sequence 
      of hsa-miR-137-3p, and dual luciferase reporter assay indicates the potential 
      binding capacity of miR-137-3p to 3'-UTR of PTP4A3 and KCNQ1OT1. We found that 
      miR-137-3p mimic inhibited cell migration and invasion, as well as repressed 
      alterations of EMT biomarkers and critical proteins in the TGF-beta signaling 
      pathway. Rescue experiment results revealed that co-transfection of miR-137-3p 
      mimic and PTP4A3-WT plasmid reversed these changes following transfection with 
      miR-137-3p mimic alone. We found that KCNQ1OT1 was predominantly distributed in 
      the cytoplasm from a subcellular fractionation assay. Functionally, silencing of 
      KCNQ1OT1 repressed cell migration and invasion, and caused alterations of EMT 
      biomarkers and critical proteins in the TGF-beta signaling pathway, which were all 
      restored by co-transfection with anti-miR-137-3p or PTP4A3-WT plasmid. 
      Furthermore, overexpression of miR-137-3p or silencing of KCNQ1OT1 dramatically 
      rescued the effects of endosulfan on promoting cell migration and invasion. These 
      findings suggest that endosulfan can indeed promote cell migration and invasion 
      via the KCNQ1OT1/miR-137-3p/PTP4A3 axis in PCa cells.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Wang, Yue
AU  - Wang Y
AD  - Institute of Environmental Systems Biology, Environment Science and Engineering 
      College, Dalian Maritime University, Linghai Road 1, Dalian, 116026, PR China.
FAU - Guo, Yubing
AU  - Guo Y
AD  - Institute of Environmental Systems Biology, Environment Science and Engineering 
      College, Dalian Maritime University, Linghai Road 1, Dalian, 116026, PR China.
FAU - Lu, Yanyuan
AU  - Lu Y
AD  - Institute of Environmental Systems Biology, Environment Science and Engineering 
      College, Dalian Maritime University, Linghai Road 1, Dalian, 116026, PR China.
FAU - Sun, Yeqing
AU  - Sun Y
AD  - Institute of Environmental Systems Biology, Environment Science and Engineering 
      College, Dalian Maritime University, Linghai Road 1, Dalian, 116026, PR China.
FAU - Xu, Dan
AU  - Xu D
AD  - Institute of Environmental Systems Biology, Environment Science and Engineering 
      College, Dalian Maritime University, Linghai Road 1, Dalian, 116026, PR China. 
      Electronic address: jotan1995@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220708
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (KCNQ1OT1 long non-coding RNA, human)
RN  - 0 (MIRN137 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Potassium Channels, Voltage-Gated)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 3.1.3.48 (PTP4A3 protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - OKA6A6ZD4K (Endosulfan)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Endosulfan/toxicity
MH  - Humans
MH  - Male
MH  - *MicroRNAs/genetics
MH  - Neoplasm Proteins/genetics
MH  - Potassium Channels, Voltage-Gated
MH  - *Prostatic Neoplasms
MH  - *Protein Tyrosine Phosphatases/genetics
MH  - *RNA, Long Noncoding/genetics
MH  - Transforming Growth Factor beta/metabolism
OTO - NOTNLM
OT  - Endosulfan
OT  - Invasion
OT  - KCNQ1OT1
OT  - Migration
OT  - PTP4A3
OT  - miR-137-3p
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/07/12 06:00
MHDA- 2022/09/09 06:00
CRDT- 2022/07/11 19:23
PHST- 2022/03/26 00:00 [received]
PHST- 2022/07/03 00:00 [revised]
PHST- 2022/07/05 00:00 [accepted]
PHST- 2022/07/12 06:00 [pubmed]
PHST- 2022/09/09 06:00 [medline]
PHST- 2022/07/11 19:23 [entrez]
AID - S0048-9697(22)04350-9 [pii]
AID - 10.1016/j.scitotenv.2022.157252 [doi]
PST - ppublish
SO  - Sci Total Environ. 2022 Nov 1;845:157252. doi: 10.1016/j.scitotenv.2022.157252. 
      Epub 2022 Jul 8.