PMID- 35819336
OWN - NLM
STAT- MEDLINE
DCOM- 20220728
LR  - 20230728
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 70
IP  - 29
DP  - 2022 Jul 27
TI  - Detection of Acrylamide in Foodstuffs by Nanobody-Based Immunoassays.
PG  - 9179-9186
LID - 10.1021/acs.jafc.2c01872 [doi]
AB  - Acrylamide is toxic aliphatic amide formed via the Maillard reaction between 
      asparagine and reducing sugars during thermal processing of food. Herein, a 
      specific nanobody termed Nb-7E against the acrylamide derivative xanthyl 
      acrylamide (XAA) was isolated from an immunized phage display library and 
      confirmed to be able to detect acrylamide. First, an indirect competitive 
      enzyme-linked immunosorbent assay (ic-ELISA) was established for acrylamide with 
      a limit of detection (LOD) of 0.089 mug/mL and working range from 0.23 to 5.6 
      mug/mL. Furthermore, an enhanced electrochemical immunoassay (ECIA) was developed 
      based on the optimized reaction conditions. The LOD was as low as 0.033 mug/mL, 
      threefold improved compared to that of ic-ELISA, and a wider linear detection 
      range from 0.39 to 50.0 mug/mL was achieved. The average recoveries ranged from 
      88.29 to 111.76% in spiked baked biscuits and potato crisps. Finally, the 
      analytical performance of the ECIA was validated by standard ultraperformance 
      liquid chromatography tandem mass spectrometry (UPLC-MS/MS).
FAU - Liang, Yifan
AU  - Liang Y
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Zeng, Yuyao
AU  - Zeng Y
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Luo, Lin
AU  - Luo L
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Xu, Zhenlin
AU  - Xu Z
AUID- ORCID: 0000-0002-1197-4615
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
AD  - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
FAU - Shen, Yudong
AU  - Shen Y
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
FAU - Wang, Hong
AU  - Wang H
AUID- ORCID: 0000-0001-9136-8959
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou 510642, China.
AD  - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
FAU - Hammock, Bruce D
AU  - Hammock BD
AUID- ORCID: 0000-0003-1408-8317
AD  - Department of Entomology and Nematology, UCD Comprehensive Cancer Center, 
      University of California, Davis, California 95616, United States.
LA  - eng
GR  - P42 ES004699/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20220712
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 20R035KLCI (Acrylamide)
SB  - IM
MH  - *Acrylamide/analysis
MH  - Chromatography, Liquid
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Food Contamination/analysis
MH  - *Tandem Mass Spectrometry/methods
PMC - PMC10111249
MID - NIHMS1890005
OTO - NOTNLM
OT  - ELISA
OT  - acrylamide
OT  - electrochemical immunoassay
OT  - nanobody
COIS- The authors declare no competing financial interest.
EDAT- 2022/07/13 06:00
MHDA- 2022/07/29 06:00
PMCR- 2023/07/27
CRDT- 2022/07/12 10:12
PHST- 2022/07/13 06:00 [pubmed]
PHST- 2022/07/29 06:00 [medline]
PHST- 2022/07/12 10:12 [entrez]
PHST- 2023/07/27 00:00 [pmc-release]
AID - 10.1021/acs.jafc.2c01872 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2022 Jul 27;70(29):9179-9186. doi: 10.1021/acs.jafc.2c01872. 
      Epub 2022 Jul 12.