PMID- 35820160
OWN - NLM
STAT- MEDLINE
DCOM- 20220728
LR  - 20220728
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 70
IP  - 29
DP  - 2022 Jul 27
TI  - Versatile Application of Nanobodies for Food Allergen Detection and Allergy 
      Immunotherapy.
PG  - 8901-8912
LID - 10.1021/acs.jafc.2c03324 [doi]
AB  - The unique characteristics of camelid heavy-chain only antibody (HCAb) derived 
      nanobodies (Nbs) have facilitated their employment as tools for research and 
      application in extensive fields including food safety inspection, diagnosis and 
      therapy of diseases, etc., to develop immune detecting techniques or alternative 
      candidates of conventional antibodies as diagnostic and therapeutic reagents. The 
      wide application in the fields of food allergen inspection and immunotherapy has 
      not been addressed as not much results published in the literature. The robust 
      properties and straightforward selecting strategy of Nbs impel the advantageous 
      employment compared with monoclonal antibodies (mAbs) to establish immunoassay 
      and serve as blocking antibodies to compete immunoglobulin E (IgE) binding 
      epitopes on food allergens. More and more efforts have been invested to develop 
      specific Nbs against food allergen proteins, such as macadamia allergen of Mac i 
      1, peanut allergen of Ara h 3, and lupine allergen of Lup an 1, which 
      demonstrated the potential of Nbs for research and application in food allergen 
      surveillance. Meanwhile, the paratopes of Nbs preferably targeting the unique 
      epitopes of food allergens can provide more possibilities to serve as blocking 
      antibodies to shield IgE binding epitopes for food allergy immunotherapy. 
      Regardless, the research and application of Nbs in the field of food allergen and 
      allergic reactions are expected to attract dramatic focus and produce promising 
      research outputs.
FAU - Hu, Yaozhong
AU  - Hu Y
AUID- ORCID: 0000-0002-6228-9220
AD  - Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai 
      University, Tianjin 300071, China.
FAU - Wang, Yi
AU  - Wang Y
AD  - Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai 
      University, Tianjin 300071, China.
FAU - Lin, Jing
AU  - Lin J
AD  - Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai 
      University, Tianjin 300071, China.
FAU - Wu, Sihao
AU  - Wu S
AD  - Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai 
      University, Tianjin 300071, China.
FAU - Muyldermans, Serge
AU  - Muyldermans S
AUID- ORCID: 0000-0002-3678-3575
AD  - Cellular and Molecular Immunology, Vrije Universiteit Brussel, 1050 Brussels, 
      Belgium.
FAU - Wang, Shuo
AU  - Wang S
AUID- ORCID: 0000-0003-0910-6146
AD  - Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai 
      University, Tianjin 300071, China.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20220712
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Blocking)
RN  - 0 (Antigens, Plant)
RN  - 0 (Epitopes)
RN  - 0 (Immunologic Factors)
RN  - 0 (Single-Domain Antibodies)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens
MH  - Antibodies, Blocking
MH  - Antigens, Plant
MH  - Epitopes
MH  - *Food Hypersensitivity/therapy
MH  - Humans
MH  - Immunoglobulin E
MH  - Immunologic Factors
MH  - Immunotherapy/methods
MH  - *Single-Domain Antibodies
OTO - NOTNLM
OT  - food allergen
OT  - food allergy
OT  - immunoassay
OT  - immunotherapy
OT  - nanobody
EDAT- 2022/07/13 06:00
MHDA- 2022/07/29 06:00
CRDT- 2022/07/12 17:03
PHST- 2022/07/13 06:00 [pubmed]
PHST- 2022/07/29 06:00 [medline]
PHST- 2022/07/12 17:03 [entrez]
AID - 10.1021/acs.jafc.2c03324 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2022 Jul 27;70(29):8901-8912. doi: 10.1021/acs.jafc.2c03324. 
      Epub 2022 Jul 12.