PMID- 35820953
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1749-8546 (Print)
IS  - 1749-8546 (Electronic)
IS  - 1749-8546 (Linking)
VI  - 17
IP  - 1
DP  - 2022 Jul 12
TI  - Bu Shen Huo Xue decoction promotes functional recovery in spinal cord injury mice 
      by improving the microenvironment to promote axonal regeneration.
PG  - 85
LID - 10.1186/s13020-022-00639-y [doi]
LID - 85
AB  - BACKGROUND: Bu-Shen-Huo-Xue (BSHX) decoction has been used in the postoperative 
      rehabilitation of patients with spinal cord injury in China. In the present 
      study, we aim to reveal the bioactive compounds in BSHX decoction and 
      comprehensively explore the effects of BSHX decoction and the underlying 
      mechanism in spinal cord injury recovery. METHODS: The main chemical constituents 
      in BSHX decoction were determined by UPLC-MS/MS. SCI mice were induced by a 
      pneumatic impact device at T9-T10 level of the vertebra, and treated with BSHX 
      decoction. Basso-Beattie-Bresnahan (BBB) score, footprint analysis, 
      hematoxylin-eosin (H&E) staining, Nissl staining and a series of 
      immunofluorescence staining were performed to investigate the functional 
      recovery, glial scar formation and axon regeneration after BSHX treatment. 
      Immunofluorescent staining of bromodeoxyuridine (BrdU), neuronal nuclei (NeuN) 
      and glial fibrillary acidic protein (GFAP) was performed to evaluate the effect 
      of BSHX decoction on neural stem cells (NSCs) proliferation and differentiation. 
      RESULTS: We found that the main compounds in BSHX decoction were Gallic acid, 
      3,4-Dihydroxybenzaldehyde, (+)-Catechin, Paeoniflorin, Rosmarinic acid, and 
      Diosmetin. BSHX decoction improved the pathological findings in SCI mice through 
      invigorating blood circulation and cleaning blood stasis in the lesion site. In 
      addition, it reduced tissue damage and neuron loss by inhibiting astrocytes 
      activation, and promoting the polarization of microglia towards M2 phenotype. The 
      functional recovery test revealed that BSHX treatment improved the motor function 
      recovery post SCI. CONCLUSIONS: Our study provided evidence that BSHX treatment 
      could improve the microenvironment of the injured spinal cord to promote axonal 
      regeneration and functional recovery in SCI mice.
CI  - (c) 2022. The Author(s).
FAU - Hou, Yonghui
AU  - Hou Y
AD  - Department of Orthopedic Surgery, Guangdong Provincial Hospital of Chinese 
      Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese 
      Medicine, No. 111 Dade Road, Guangzhou, 510120, Guangdong, People's Republic of 
      China.
AD  - Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Baiyun District, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
FAU - Luo, Dan
AU  - Luo D
AD  - Department of Orthopedic Surgery, Guangdong Provincial Hospital of Chinese 
      Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese 
      Medicine, No. 111 Dade Road, Guangzhou, 510120, Guangdong, People's Republic of 
      China.
AD  - Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Baiyun District, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
FAU - Hou, Yu
AU  - Hou Y
AD  - Department of Orthopedic Surgery, Guangdong Provincial Hospital of Chinese 
      Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese 
      Medicine, No. 111 Dade Road, Guangzhou, 510120, Guangdong, People's Republic of 
      China.
AD  - Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Baiyun District, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
FAU - Luan, Jiyao
AU  - Luan J
AD  - Department of Orthopedic Surgery, Guangdong Provincial Hospital of Chinese 
      Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese 
      Medicine, No. 111 Dade Road, Guangzhou, 510120, Guangdong, People's Republic of 
      China.
AD  - Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Baiyun District, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
FAU - Zhan, Jiheng
AU  - Zhan J
AD  - Department of Orthopedic Surgery, Guangdong Provincial Hospital of Chinese 
      Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese 
      Medicine, No. 111 Dade Road, Guangzhou, 510120, Guangdong, People's Republic of 
      China.
AD  - Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Baiyun District, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
FAU - Chen, Zepeng
AU  - Chen Z
AD  - Department of Orthopedic Surgery, Guangdong Provincial Hospital of Chinese 
      Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese 
      Medicine, No. 111 Dade Road, Guangzhou, 510120, Guangdong, People's Republic of 
      China.
AD  - Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Baiyun District, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
FAU - E, Shunmei
AU  - E S
AD  - Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Baiyun District, 
      Guangzhou, 510405, Guangdong, People's Republic of China.
AD  - Department of Laboratory Medicine, Guangdong Provincial Hospital of Chinese 
      Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese 
      Medicine, Guangzhou Higher Education Mega Center, 55 Neihuan Xi Road, Panyu 
      District, Guangzhou, 510120, Guangdong, People's Republic of China.
FAU - Xu, Liangliang
AU  - Xu L
AD  - Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Baiyun District, 
      Guangzhou, 510405, Guangdong, People's Republic of China. xull-2016@gzucm.edu.cn.
AD  - Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, 
      Guangzhou, 510405, Guangdong, People's Republic of China. xull-2016@gzucm.edu.cn.
AD  - Key Laboratory of Orthopaedics & Traumatology, The First Affiliated Hospital of 
      Guangzhou University of Chinese Medicine, Guangzhou University of Chinese 
      Medicine, Guangzhou, People's Republic of China. xull-2016@gzucm.edu.cn.
FAU - Lin, Dingkun
AU  - Lin D
AD  - Department of Orthopedic Surgery, Guangdong Provincial Hospital of Chinese 
      Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese 
      Medicine, No. 111 Dade Road, Guangzhou, 510120, Guangdong, People's Republic of 
      China. lindingkuntcm@126.com.
AD  - Guangzhou University of Chinese Medicine, No. 12, Jichang Road, Baiyun District, 
      Guangzhou, 510405, Guangdong, People's Republic of China. lindingkuntcm@126.com.
LA  - eng
GR  - 81673992/National Natural Science Foundation of China/
GR  - 82074451/National Natural Science Foundation of China/
GR  - 20221161/Research Project of Traditional Chinese Medicine Bureau of Guangdong 
      Province/
GR  - 202102010203/Science and Technology Program of Guangzhou, China/
GR  - 2021M700905/Postdoctoral Research Foundation of China/
PT  - Journal Article
DEP - 20220712
PL  - England
TA  - Chin Med
JT  - Chinese medicine
JID - 101265109
PMC - PMC9277908
OTO - NOTNLM
OT  - Axonal regeneration
OT  - Bu-Shen-Huo-Xue decoction
OT  - Inflammatory response
OT  - Microenvironment
OT  - Spinal cord injury
COIS- The authors declare no competing interests.
EDAT- 2022/07/13 06:00
MHDA- 2022/07/13 06:01
PMCR- 2022/07/12
CRDT- 2022/07/12 23:40
PHST- 2022/01/10 00:00 [received]
PHST- 2022/06/28 00:00 [accepted]
PHST- 2022/07/12 23:40 [entrez]
PHST- 2022/07/13 06:00 [pubmed]
PHST- 2022/07/13 06:01 [medline]
PHST- 2022/07/12 00:00 [pmc-release]
AID - 10.1186/s13020-022-00639-y [pii]
AID - 639 [pii]
AID - 10.1186/s13020-022-00639-y [doi]
PST - epublish
SO  - Chin Med. 2022 Jul 12;17(1):85. doi: 10.1186/s13020-022-00639-y.