PMID- 35821149
OWN - NLM
STAT- MEDLINE
DCOM- 20221014
LR  - 20221014
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 167
IP  - 11
DP  - 2022 Nov
TI  - Characterization of replication and variations in genome segments of a bat 
      reovirus, BatMRV/B19-02, by RNA-seq in infected Vero-E6 cells.
PG  - 2133-2142
LID - 10.1007/s00705-022-05534-3 [doi]
AB  - Mammalian orthoreoviruses (MEVs) that can cause enteric, respiratory, and 
      encephalitic infections have been identified in a wide variety of mammalian 
      species. Here, we report a novel MRV type 1 strain detected in Miniopterus 
      schreibersii that may have resulted from reassortment events. Using 
      next-generation RNA sequencing (RNA-seq), we found that the ratios of the RNA 
      levels of the 10 reovirus segments in infected cells were constant during the 
      late stages of infection. We also discovered that the relative abundance of each 
      segment differed. Notably, the relative abundance of M2 (encoding the micro1 protein) 
      and S4 (encoding the sigma3 protein) RNAs was higher than that of the others 
      throughout the infection. Additionally, massive junctions were identified. These 
      results support the hypothesis that defective genome segments are generated and 
      that cross-family recombination occurs. These data may further the study of gene 
      function, viral replication, and virus evolution.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, 
      part of Springer Nature.
FAU - Lo, Van Thi
AU  - Lo VT
AD  - Bionanotechnology Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Daejeon, Korea.
AD  - Bio-Analytical Science Division, Korea University of Science and Technology 
      (UST), Daejeon, Korea.
FAU - Yoon, Sun-Woo
AU  - Yoon SW
AD  - Bionanotechnology Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Daejeon, Korea.
AD  - Bio-Analytical Science Division, Korea University of Science and Technology 
      (UST), Daejeon, Korea.
FAU - Noh, Ji Yeong
AU  - Noh JY
AD  - Department of Biological Sciences and Biotechnology, College of Natural Science, 
      Chungbuk National University, Cheongju, Korea.
FAU - Jang, Seong Sik
AU  - Jang SS
AD  - Department of Biological Sciences and Biotechnology, College of Natural Science, 
      Chungbuk National University, Cheongju, Korea.
FAU - Na, Woonsung
AU  - Na W
AD  - College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
FAU - Song, Daesub
AU  - Song D
AD  - College of Veterinary Medicine, Seoul National University, Seoul, Korea.
FAU - Jeong, Dae Gwin
AU  - Jeong DG
AD  - Bionanotechnology Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Daejeon, Korea. dgjeong@kribb.re.kr.
AD  - Bio-Analytical Science Division, Korea University of Science and Technology 
      (UST), Daejeon, Korea. dgjeong@kribb.re.kr.
FAU - Kim, Hye Kwon
AU  - Kim HK
AUID- ORCID: 0000-0003-3458-3403
AD  - Department of Biological Sciences and Biotechnology, College of Natural Science, 
      Chungbuk National University, Cheongju, Korea. khk1329@chungbuk.ac.kr.
LA  - eng
GR  - H-GUARD_2013M3A6B2078954/Ministry of Science and ICT/
GR  - NRF-2020R1C1C1010440/National Research Foundation of Korea/
GR  - 2020R1A6A1A06046235/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20220712
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - *Chiroptera
MH  - Genome, Viral
MH  - *Orthoreovirus/genetics
MH  - RNA
MH  - RNA-Seq
MH  - *Reoviridae/genetics
EDAT- 2022/07/13 06:00
MHDA- 2022/10/15 06:00
CRDT- 2022/07/12 23:56
PHST- 2022/04/07 00:00 [received]
PHST- 2022/05/26 00:00 [accepted]
PHST- 2022/07/13 06:00 [pubmed]
PHST- 2022/10/15 06:00 [medline]
PHST- 2022/07/12 23:56 [entrez]
AID - 10.1007/s00705-022-05534-3 [pii]
AID - 10.1007/s00705-022-05534-3 [doi]
PST - ppublish
SO  - Arch Virol. 2022 Nov;167(11):2133-2142. doi: 10.1007/s00705-022-05534-3. Epub 
      2022 Jul 12.