PMID- 35822680
OWN - NLM
STAT- MEDLINE
DCOM- 20220714
LR  - 20220714
IS  - 1735-5249 (Electronic)
IS  - 1735-1502 (Linking)
VI  - 21
IP  - 3
DP  - 2022 Jun 18
TI  - The Effect of Age and Cell Culture Parameters on the Quantity and Function of 
      Bone Marrow-derived Dendritic Cells.
PG  - 300-312
LID - 10.18502/ijaai.v21i3.9803 [doi]
AB  - Dendritic cells (DCs) are a group of bone marrow-derived cells that play a 
      crucial role in innate and acquired immune responses. Bone marrow-derived 
      dendritic cells (BMDC) are used in many studies, so the efficiency and purity of 
      the differentiated cells are essential. This study aimed to investigate the 
      effect of several parameters, including the age of mice, cell culture medium, and 
      swirling of the culture plate, to increase the efficiency of the induced cells, 
      considering the standard protocols. Bone marrow-derived dendritic cells were 
      induced from both juvenile and adult mice bone marrow cells. Then, the purity of 
      CD11c+ cells was compared between juvenile mice BMDCs and adult mice BMDCs. Cells 
      were cultured in an enriched and non-enriched medium, and some wells were swirled 
      when changing the medium on the 3rd day. Then the effect of enriched medium and 
      swirling before medium replacement were evaluated based on the expression of the 
      CD11c marker. The efficiency of DCs differentiation (CD11c+ cells) was higher 
      when juvenile mouse bone marrow precursors were used compared to adult mice; 
      using the enriched media with supplements and swirling the well before media 
      replacement significantly affected the purity of immature CD11c+ cells. Due to 
      our results, using juvenile mice, an enriched culture medium, and physical 
      removal of granulocyte cells could significantly improve the purity and 
      efficiency of CD11c+ cells. Therefore, considering these three items in the 
      production protocol of these cells can probably reduce the use of 
      lymphocyte-removing antibodies and purification methods.
FAU - Golara, Maryam
AU  - Golara M
AD  - Department of Immunology, Faculty of Medicine, Tarbiat Modarres University, 
      Tehran, Iran. golimmuno@yahoo.com.
FAU - Amiri, Mohammad Mehdi
AU  - Amiri MM
AD  - Department of Immunology, School of Public Health, Tehran University of Medical 
      Sciences, Tehran, Iran. golimmuno@yahoo.com.
FAU - Moazzeni, Seyed Mohammad
AU  - Moazzeni SM
AD  - Department of Immunology, Faculty of Medical Sciences, Tarbiat Modarres 
      University, Tehran, Iran. moazzeni@modares.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20220618
PL  - Iran
TA  - Iran J Allergy Asthma Immunol
JT  - Iranian journal of allergy, asthma, and immunology
JID - 101146178
RN  - 0 (CD11c Antigen)
SB  - IM
MH  - Animals
MH  - *Bone Marrow/metabolism
MH  - CD11c Antigen/metabolism
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - *Dendritic Cells
MH  - Mice
MH  - Mice, Inbred C57BL
OTO - NOTNLM
OT  - Bone marrow cells
OT  - Cell differentiation
OT  - Dendritic cells
OT  - Primary cell culture
EDAT- 2022/07/14 06:00
MHDA- 2022/07/15 06:00
CRDT- 2022/07/13 07:37
PHST- 2022/01/27 00:00 [received]
PHST- 2022/04/26 00:00 [accepted]
PHST- 2022/07/13 07:37 [entrez]
PHST- 2022/07/14 06:00 [pubmed]
PHST- 2022/07/15 06:00 [medline]
AID - 10.18502/ijaai.v21i3.9803 [doi]
PST - epublish
SO  - Iran J Allergy Asthma Immunol. 2022 Jun 18;21(3):300-312. doi: 
      10.18502/ijaai.v21i3.9803.