PMID- 35832542
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 12
DP  - 2022
TI  - Angiofibroma of Soft Tissue: A Clinicopathological Study of Eight Cases With 
      Emphasis on the Diagnostic Utility of Fluorescence In Situ Hybridization 
      Detection for NCOA2 Rearrangement.
PG  - 900411
LID - 10.3389/fonc.2022.900411 [doi]
LID - 900411
AB  - BACKGROUND: Angiofibroma of soft tissue (AFST) is a rare mesenchymal neoplasm of 
      fibroblastic differentiation. Due to its diverse morphology and the lack of 
      specific immunohistochemistry (IHC) markers, AFST could elicit a broad range of 
      differential diagnosis. Several studies have disclosed in AFST recurrent gene 
      fusions involving NCOA2, mainly AHRR-NCOA2 fusion, providing a useful approach to 
      diagnosing this lesion. We report eight additional cases of this rare entity with 
      emphasis on the diagnostic utility of fluorescence in situ hybridization (FISH) 
      detection for NCOA2 rearrangement. METHODS: Clinicopathological data for eight 
      AFSTs were retrieved. IHC was performed, and FISH was used to detect 
      rearrangements involving NCOA2, DDIT3, and FUS loci. RESULTS: There were five 
      female and three male patients, ranging in age from 29 to 69 years (median: 55 
      years). The patients presented mostly with a slow-growing mass in the 
      extremities, with or without intermittent pain. All tumors were located in the 
      lower extremities with three (27.5%) involving or adjacent to the knee joints. 
      Tumor size ranged from 1.5 to 3.8 cm (median: 3.0 cm). Morphologically, the 
      tumors consisted of a proliferation of uniform, bland spindle cells set in 
      alternating myxoid and collagenous stroma with a prominent vascular network 
      composed of countless small, branching, thin-walled blood vessels. Foci of 
      "chicken wire"-like capillaries and medium- to large-sized blood vessels with 
      prominent staghorn morphology were evident in two and four cases, respectively. 
      In addition, sheets of small round cells and foci of cystic changes were observed 
      in one each case. Degenerative nuclear atypia was identified in three cases, 
      while mitosis and tumor necrosis were absent. By IHC, the stromal cells were 
      variably positive for epithelial membrane antigen, desmin, and CD68. By FISH 
      analysis, seven out of eight cases (87.5%) showed NCOA2 rearrangement, and the 
      remaining one had increased gene copy numbers of intact NCOA2; rearrangements 
      involving FUS (0/4) and DDIT3 (0/3) were not identified in the cases analyzed. 
      All tumors were surgically removed, and none had recurrence at follow-up from 5 
      to 73 months. CONCLUSIONS: FISH analysis for NCOA2 rearrangement represents a 
      practical method for confirming the diagnosis of AFST on the basis of appropriate 
      histomorphological backgrounds.
CI  - Copyright (c) 2022 Wang, Fan, Wei, Xu, Ru and Zhao.
FAU - Wang, Canming
AU  - Wang C
AD  - Department of Pathology, Cancer Hospital of the University of Chinese Academy of 
      Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer, 
      Chinese Academy of Sciences, Hangzhou, China.
FAU - Fan, Yuqian
AU  - Fan Y
AD  - Cancer Center, Department of Pathology, Zhejiang Provincial People's Hospital, 
      Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, China.
FAU - Wei, Jianguo
AU  - Wei J
AD  - Department of Pathology, Shaoxing People's Hospital, Shaoxing, China.
FAU - Xu, Qiujie
AU  - Xu Q
AD  - Department of Pathology, Tongxiang Second People's Hospital, Jiaxing, China.
FAU - Ru, Guoqing
AU  - Ru G
AD  - Cancer Center, Department of Pathology, Zhejiang Provincial People's Hospital, 
      Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, China.
FAU - Zhao, Ming
AU  - Zhao M
AD  - Cancer Center, Department of Pathology, Zhejiang Provincial People's Hospital, 
      Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20220627
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC9271777
OTO - NOTNLM
OT  - AHRR-NCOA2 fusion
OT  - NCOA2 rearrangement
OT  - angiofibroma of soft tissue
OT  - differential diagnosis
OT  - fluorescence in situ hybridization
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/15 06:00
MHDA- 2022/07/15 06:01
PMCR- 2022/01/01
CRDT- 2022/07/14 02:25
PHST- 2022/03/20 00:00 [received]
PHST- 2022/05/25 00:00 [accepted]
PHST- 2022/07/14 02:25 [entrez]
PHST- 2022/07/15 06:00 [pubmed]
PHST- 2022/07/15 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2022.900411 [doi]
PST - epublish
SO  - Front Oncol. 2022 Jun 27;12:900411. doi: 10.3389/fonc.2022.900411. eCollection 
      2022.