PMID- 35835386
OWN - NLM
STAT- MEDLINE
DCOM- 20220817
LR  - 20220817
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 127
DP  - 2022 Aug
TI  - Toxicological effects of polystyrene nanoplastics and perfluorooctanoic acid to 
      Gambusia affinis.
PG  - 1100-1112
LID - S1050-4648(22)00382-5 [pii]
LID - 10.1016/j.fsi.2022.06.064 [doi]
AB  - Plastic pollution has attracted huge attention from public and scientific 
      community in recent years. In the environment, nanoplastics (NPs, <100 nm) can 
      interact with persistent organic pollutants (POPs) such as perfluorooctanoic acid 
      (PFOA) and may exacerbate associated toxic impacts. The present study aims to 
      explore the single and combined ecotoxicological effects of PFOA and polystyrene 
      nanoplastics (PS-NPs, 80 nm) on the PI3K/AKT3 signaling pathway using a 
      freshwater fish model Gambusia affinis. Fish were exposed individually to PS-NPs 
      (200 mug/L) and PFOA (50, 500, 5000 mug/L) and their chemical mixtures for 96 h. 
      Our results showed that the co-exposure significantly altered the mRNA relative 
      expression of PI3K, AKT3, IKKbeta and IL-1beta, compared to corresponding single 
      exposure and control groups, indicating that the PFOA-NP co-exposure can activate 
      the PI3K/AKT3 signaling pathway. The bioinformatic analyses showed that AKT3 had 
      more probes and exhibited a significantly sensitive correlation with DNA 
      methylation, compared to other genes (PIK3CA, IKBKB, and IL1B). Further, the mRNA 
      expressions of PIK3CA, AKT3, and IKBKB had a significant correlation with copy 
      number variation (CNV) in human liver hepatocellular carcinoma (LIHC). And PIK3CA 
      had the highest mutation rate among other genes of interest for LIHC. Moreover, 
      AKT3 showed a relatively lower expression in TAM and CAF cells, compared to 
      PIK3CA, IKBKB, and IL1B. Besides, hsa-mir-155-5p was closely correlated with 
      AKT3, PIK3CA, IKBKB, and IL1B. In summary, these results provide evidence that 
      NPs could enhance the carcinogenic effects of POPs on aquatic organisms and 
      highlight possible targets of LIHC induced by PFOA-NP co-exposure.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Liu, Shulin
AU  - Liu S
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      510642, China.
FAU - Yan, Lei
AU  - Yan L
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      510642, China.
FAU - Zhang, Yanling
AU  - Zhang Y
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      510642, China.
FAU - Junaid, Muhammad
AU  - Junaid M
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      510642, China.
FAU - Wang, Jun
AU  - Wang J
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      510642, China; Institute of Eco-Environmental Research, Guangxi Academy of 
      Sciences, Nanning, 530007, China; Southern Marine Science and Engineering 
      Guangdong Laboratory, Zhuhai, 528478, China. Electronic address: 
      wangjun2016@scau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220711
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Caprylates)
RN  - 0 (Fluorocarbons)
RN  - 0 (Microplastics)
RN  - 0 (Polystyrenes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - 947VD76D3L (perfluorooctanoic acid)
RN  - EC 2.7.1.137 (Class I Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
SB  - IM
MH  - Animals
MH  - Caprylates
MH  - Class I Phosphatidylinositol 3-Kinases
MH  - *Cyprinodontiformes/genetics/metabolism
MH  - DNA Copy Number Variations
MH  - Fluorocarbons
MH  - Humans
MH  - I-kappa B Kinase
MH  - Microplastics/toxicity
MH  - *Nanoparticles/chemistry
MH  - Phosphatidylinositol 3-Kinases
MH  - Polystyrenes/toxicity
MH  - RNA, Messenger
MH  - *Water Pollutants, Chemical/metabolism
OTO - NOTNLM
OT  - Liver hepatocellular carcinoma
OT  - Nanoplastics
OT  - PI3K/AKT3 signaling pathway
OT  - Perfluorooctanoic acid
OT  - TCGA
EDAT- 2022/07/15 06:00
MHDA- 2022/08/18 06:00
CRDT- 2022/07/14 19:37
PHST- 2022/05/18 00:00 [received]
PHST- 2022/06/22 00:00 [revised]
PHST- 2022/06/27 00:00 [accepted]
PHST- 2022/07/15 06:00 [pubmed]
PHST- 2022/08/18 06:00 [medline]
PHST- 2022/07/14 19:37 [entrez]
AID - S1050-4648(22)00382-5 [pii]
AID - 10.1016/j.fsi.2022.06.064 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2022 Aug;127:1100-1112. doi: 10.1016/j.fsi.2022.06.064. 
      Epub 2022 Jul 11.