PMID- 35836842
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 14
IP  - 6
DP  - 2022
TI  - LncRNA MALAT1 inhibits the proliferation and invasiveness of laryngeal squamous 
      cell carcinoma Hep-2 cells by modulating miR-362-3p.
PG  - 3729-3740
AB  - OBJECTIVE: To investigate the mechanism of lncRNA MALAT1 (MALAT1) inhibiting the 
      proliferation and invasiveness of laryngeal squamous cell carcinoma (LSCC) Hep-2 
      cells by modulating miR-362-3p. METHODS: We collected the expression profile of 
      lncRNAs and miRNAs in LSCC downloaded from The Cancer Genome Atlas (TCGA) 
      database as well as LSCC tissue samples and adjacent normal counterparts resected 
      from LSCC patients in Lvliang People's Hospital and First Hospital of Shanxi 
      Medical University between January 2018 and June 2020 for analysis. Human LSCC 
      Hep-2 cells were selected for experiments. The expression of miR-362-3p and 
      MALAT1 was detected by quantitative real-time polymerase chain reaction 
      (qRT-PCR). Cells were subsequently transfected to knock out MALAT1, and the 
      growth, metastasis and invasiveness of cells were evaluated by CCK-8 assay, plate 
      clone formation, wound healing, and Transwell invasion assays respectively. The 
      binding of MALAT1 to miR-362-3p was verified by RNA pull-down, RNA binding 
      protein immunoprecipitation (RIP), and dual-luciferase reporter assays. RESULTS: 
      MALAT1 was highly expressed while miR-362-3p was lowly expressed in both LSCC 
      tissues and cells compared with normal counterparts. MALAT1 knockdown inhibited 
      the viability of Hep-2 cells, reducing the number of plate clone-forming cells as 
      well as the number of migrated and invaded cells. Transfection of miR-362-3p 
      inhibitor into Hep-2 cells treated by si-MALAT1 reversed the inhibition of 
      si-MALAT1 on the proliferation of Hep-2 cells, and promoted cell invasiveness and 
      migration. MALAT1 can sponge miR-362-3p and inhibit its expression. CONCLUSIONS: 
      Knockdown of MALAT can inhibit Hep-2 cell proliferation and reduce its 
      invasiveness and migration by modulating miR-362-3p.
CI  - AJTR Copyright (c) 2022.
FAU - Xie, Baotong
AU  - Xie B
AD  - Department of Otolaryngology, Lvliang People's Hospital, The Affiliated Lvliang 
      Hospital of Shanxi Medical University Lvliang, Shanxi, China.
FAU - Wang, Zhaohua
AU  - Wang Z
AD  - Department of Otolaryngology, Lvliang People's Hospital, The Affiliated Lvliang 
      Hospital of Shanxi Medical University Lvliang, Shanxi, China.
FAU - Li, Tao
AU  - Li T
AD  - Department of Ophthalmology, Lvliang People's Hospital, The Affiliated Lvliang 
      Hospital of Shanxi Medical University Lvliang, Shanxi, China.
FAU - Xue, Junfang
AU  - Xue J
AD  - Department of Otolaryngology, Lvliang People's Hospital, The Affiliated Lvliang 
      Hospital of Shanxi Medical University Lvliang, Shanxi, China.
FAU - Zhang, Chunming
AU  - Zhang C
AD  - Department of Otolaryngology and Head-neck Surgery, First Hospital of Shanxi 
      Medical University Taiyuan, Shanxi, China.
LA  - eng
PT  - Journal Article
DEP - 20220615
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC9274578
OTO - NOTNLM
OT  - LncRNA MALAT1
OT  - invasive ability
OT  - laryngeal squamous cell carcinoma
OT  - miR-362-3p
OT  - proliferative activity
COIS- None.
EDAT- 2022/07/16 06:00
MHDA- 2022/07/16 06:01
PMCR- 2022/06/15
CRDT- 2022/07/15 02:31
PHST- 2021/11/24 00:00 [received]
PHST- 2022/04/06 00:00 [accepted]
PHST- 2022/07/15 02:31 [entrez]
PHST- 2022/07/16 06:00 [pubmed]
PHST- 2022/07/16 06:01 [medline]
PHST- 2022/06/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2022 Jun 15;14(6):3729-3740. eCollection 2022.