PMID- 35837374
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1927-1220 (Electronic)
IS  - 1927-1212 (Print)
IS  - 1927-1212 (Linking)
VI  - 11
IP  - 3
DP  - 2022 Jun
TI  - Conventional Karyotyping and Fluorescence In Situ Hybridization for Detection of 
      Chromosomal Abnormalities in Multiple Myeloma.
PG  - 87-91
LID - 10.14740/jh1007 [doi]
AB  - BACKGROUND: Multiple myeloma (MM) is a genetically heterogeneous disease, with 
      cytogenetic findings that determine disease behavior. Genetic abnormalities can 
      be assessed by fluorescence in situ hybridization (FISH) analysis and/or G-banded 
      karyotyping. The two methods produce unique and overlapping information, and the 
      clinical utility of using both is investigated here. METHODS: Seventy patients 
      diagnosed with MM at a hospital in Southern California were retrospectively 
      reviewed for the FISH and G-banded karyotyping results obtained from bone marrow 
      specimens. RESULTS: Karyotype was normal in 71% (50/70), abnormal in 27% (19/70), 
      and inadequate in 1% (1/70). Among patients with abnormal karyotype, FISH 
      provided additional information about genetic aberrations in 95% of cases 
      (18/19). Among cases with abnormal FISH, karyotype provided additional 
      information about genetic aberrations in 27% of cases (18/66). When numerical 
      abnormalities were present (detected by FISH and/or karyotype), FISH detected 
      them in 95% (54/57), of which karyotype missed 70% (38/54) of the time. 
      Karyotyping detected numerical abnormalities in 33% (19/57), which FISH missed 
      16% (3/19) of the time. CONCLUSIONS: Karyotyping and FISH analysis in MM each 
      provide unique information. For most patients, performing both tests together 
      will provide more information than either test alone.
CI  - Copyright 2022, Crabtree et al.
FAU - Crabtree, Matthew
AU  - Crabtree M
AUID- ORCID: 0000-0002-3230-0599
AD  - Department of Pathology, Harbor-UCLA Medical Center, Torrance, CA 90502, USA.
FAU - Cai, Jennifer
AU  - Cai J
AUID- ORCID: 0000-0002-1877-0316
AD  - Department of Pathology, Harbor-UCLA Medical Center, Torrance, CA 90502, USA.
FAU - Qing, Xin
AU  - Qing X
AUID- ORCID: 0000-0001-8109-590X
AD  - Department of Pathology, Harbor-UCLA Medical Center, Torrance, CA 90502, USA.
LA  - eng
PT  - Journal Article
DEP - 20220627
PL  - Canada
TA  - J Hematol
JT  - Journal of hematology
JID - 101635099
PMC - PMC9275438
OTO - NOTNLM
OT  - Cytogenetics
OT  - FISH
OT  - Karyotype
OT  - Multiple myeloma
COIS- None to declare.
EDAT- 2022/07/16 06:00
MHDA- 2022/07/16 06:01
PMCR- 2022/06/27
CRDT- 2022/07/15 02:40
PHST- 2022/04/22 00:00 [received]
PHST- 2022/06/18 00:00 [accepted]
PHST- 2022/07/15 02:40 [entrez]
PHST- 2022/07/16 06:00 [pubmed]
PHST- 2022/07/16 06:01 [medline]
PHST- 2022/06/27 00:00 [pmc-release]
AID - 10.14740/jh1007 [doi]
PST - ppublish
SO  - J Hematol. 2022 Jun;11(3):87-91. doi: 10.14740/jh1007. Epub 2022 Jun 27.