PMID- 35838805 OWN - NLM STAT- MEDLINE DCOM- 20230103 LR - 20230103 IS - 1435-702X (Electronic) IS - 0721-832X (Linking) VI - 261 IP - 1 DP - 2023 Jan TI - Serum Ang-1/Ang-2 ratio may be a promising biomarker for evaluating severity of diabetic retinopathy. PG - 49-55 LID - 10.1007/s00417-022-05745-z [doi] AB - PURPOSE: To investigate the predictive role of serum angiopoietin-1 and angiopoietin-2 (Ang-1/Ang-2) in evaluating the severity of diabetic retinopathy (DR). METHODS: A total of 101 outpatients with type 2 diabetes mellitus (T2DM) were recruited and were further divided into the following five groups: T2DM without DR (non-DR), mild non-proliferative DR (NPDR), moderate NPDR, severe NPDR and proliferative DR (PDR) in accordance with the International Clinical Diabetic Retinopathy Guidelines. Furthermore, 101 serum samples were included in the further analysis using enzyme-linked immunosorbent assays. A receiver operating characteristic (ROC) curve was plotted to evaluate the diagnostic value of each index. RESULTS: The expression of Ang-1 in the PDR group was significantly lower than that in the non-DR group, while Ang-2 showed an opposite upward trend (p < 0.05). The Ang-1/Ang-2 ratio of the non-DR group was significantly lower than that of the moderate NPDR, severe NPDR and PDR (p < 0.05, p < 0.01 and p < 0.01, respectively). Differences in the Ang-1/Ang-2 ratio were observed earlier than those in the individual Ang-1 and Ang-2 measurements. The maximal Youden index was 0.512 with a calculated area under the curve (AUC) value of 0.734 (p < 0.01). CONCLUSIONS: The Ang-1/Ang-2 ratio was helpful in assessing the severity of DR and may provide potential clinical benefits as a biomarker and early warning signs for DR diagnosis. CI - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Wang, Yihan AU - Wang Y AD - Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China. FAU - Fang, Junwei AU - Fang J AD - Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China. FAU - Niu, Tian AU - Niu T AD - Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China. FAU - Xing, Xindan AU - Xing X AD - Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China. FAU - Wang, Hanying AU - Wang H AD - Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China. FAU - Shi, Xin AU - Shi X AD - Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China. FAU - Liu, Yujuan AU - Liu Y AD - Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China. FAU - Liu, Xinyi AU - Liu X AD - Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China. FAU - Chen, Chong AU - Chen C AD - Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China. cicyheart@163.com. FAU - Liu, Kun AU - Liu K AUID- ORCID: 0000-0002-0115-7160 AD - Department of Ophthalmology, Shanghai General Hospital, School of Medicine, Shanghai Jiao Tong University, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, No. 100 Haining Road, Shanghai, 20080, China. drliukun@sjtu.edu.cn. LA - eng GR - 81870667/National Natural Science Foundation of China/ GR - 81800835/National Natural Science Foundation of China/ GR - 2019YFC0840607/Key Technologies Research and Development Program of Anhui Province/ GR - 21XD1402700/Program of Shanghai Academic Research Leader/ GR - 2020084/Shanghai Talent Development Fund/ PT - Journal Article DEP - 20220715 PL - Germany TA - Graefes Arch Clin Exp Ophthalmol JT - Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie JID - 8205248 RN - 0 (Angiopoietin-1) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Diabetic Retinopathy/diagnosis MH - *Diabetes Mellitus, Type 2/complications/diagnosis MH - Angiopoietin-1 MH - Biomarkers MH - ROC Curve OTO - NOTNLM OT - Angiopoietin-1 OT - Angiopoietin-2 OT - Biomarkers OT - Diabetic retinopathy EDAT- 2022/07/16 06:00 MHDA- 2023/01/04 06:00 CRDT- 2022/07/15 11:56 PHST- 2022/04/06 00:00 [received] PHST- 2022/06/28 00:00 [accepted] PHST- 2022/06/09 00:00 [revised] PHST- 2022/07/16 06:00 [pubmed] PHST- 2023/01/04 06:00 [medline] PHST- 2022/07/15 11:56 [entrez] AID - 10.1007/s00417-022-05745-z [pii] AID - 10.1007/s00417-022-05745-z [doi] PST - ppublish SO - Graefes Arch Clin Exp Ophthalmol. 2023 Jan;261(1):49-55. doi: 10.1007/s00417-022-05745-z. Epub 2022 Jul 15.