PMID- 35839610
OWN - NLM
STAT- MEDLINE
DCOM- 20221026
LR  - 20221026
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 237
DP  - 2022 Sep
TI  - RASSF8-AS1 displays low expression in colorectal cancer and up-regulates RASSF8 
      to suppress cell invasion and migration.
PG  - 153996
LID - S0344-0338(22)00240-0 [pii]
LID - 10.1016/j.prp.2022.153996 [doi]
AB  - BACKGROUND: Colorectal cancer (CRC) is among the most prevalent cancers. Long 
      non-coding RNAs (lncRNAs) are important participant in various cancers. Based on 
      the literature, lncRNA RASSF8-AS1 inhibits laryngeal squamous cell carcinoma 
      (LSCC) malignant progression. However, the role of RASSF8-AS1 in CRC remains 
      unclear. PURPOSE: This study centered on uncovering the role of RASSF8-AS1 and 
      its related regulatory mechanisms in CRC cells. METHODS: RT-qPCR and western blot 
      were performed to examine the expression of target genes. Functional assays were 
      conducted to determine the effect of target genes on the migration and invasion 
      of CRC cells. Mechanism assays were also carried out to figure out the specific 
      downstream mechanisms of RASSF8-AS1. In vivo assays were also involved. RESULTS: 
      The expression of RASSF8-AS1 and RASSF8 was positively correlated in CRC, and the 
      two genes were down-regulated in CRC cells and tissues. Moreover, CRC cell 
      invasion and migration as well as xenograft CRC tumor growth suppressed by 
      RASSF8-AS1 overexpression were entirely recovered by RASSF8 knockdown or 
      partially rescued by miR-33a-5p augment. As for the downstream mechanism, 
      RASSF8-AS1 sponged miR-33a-5p to up-regulate RASSF8, or recruited HNRNPC to 
      stabilize RASSF8 mRNA. CONCLUSION: RASSF8-AS1 modulates miR-33a-5p/HNRNPC/RASSF8 
      axis to further impede CRC cell invasion and migration. AVAILABILITY OF DATA: The 
      research data is confidential.
CI  - Copyright (c) 2022 Elsevier GmbH. All rights reserved.
FAU - Zhang, Xi
AU  - Zhang X
AD  - Department of Anorectal, Xi'an Hospital of TCM, No.69, Fengcheng 8 Road, Xi'an, 
      Shaanxi 710021, China.
FAU - Sun, Pengjin
AU  - Sun P
AD  - Department of Anorectal, Xi'an Hospital of TCM, No.69, Fengcheng 8 Road, Xi'an, 
      Shaanxi 710021, China.
FAU - Yang, Wei
AU  - Yang W
AD  - Department of Anorectal, Xi'an Hospital of TCM, No.69, Fengcheng 8 Road, Xi'an, 
      Shaanxi 710021, China.
FAU - Li, Sizhu
AU  - Li S
AD  - Department of Anorectal, Xi'an Hospital of TCM, No.69, Fengcheng 8 Road, Xi'an, 
      Shaanxi 710021, China.
FAU - Jia, Heng
AU  - Jia H
AD  - Department of Anorectal, Xi'an Hospital of TCM, No.69, Fengcheng 8 Road, Xi'an, 
      Shaanxi 710021, China.
FAU - Wang, Wengang
AU  - Wang W
AD  - Department of Anorectal, Xi'an Hospital of TCM, No.69, Fengcheng 8 Road, Xi'an, 
      Shaanxi 710021, China.
FAU - Jiang, Shuai
AU  - Jiang S
AD  - Department of Anorectal, Xi'an Hospital of TCM, No.69, Fengcheng 8 Road, Xi'an, 
      Shaanxi 710021, China.
FAU - Wang, Lei
AU  - Wang L
AD  - Department of Anorectal, Xi'an Hospital of TCM, No.69, Fengcheng 8 Road, Xi'an, 
      Shaanxi 710021, China.
FAU - Liu, Xianglin
AU  - Liu X
AD  - Department of Traditional Chinese Medicine, Shaanxi University of Chinese 
      Medicine, Xianyang, China.
FAU - Liang, Liang
AU  - Liang L
AD  - Department of Traditional Chinese Medicine, Shaanxi University of Chinese 
      Medicine, Xianyang, China.
FAU - Zi, Wei
AU  - Zi W
AD  - Department of Anorectal, Xi'an Hospital of TCM, No.69, Fengcheng 8 Road, Xi'an, 
      Shaanxi 710021, China. Electronic address: 20861152@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20220630
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (MicroRNAs)
RN  - 0 (RASSF8 protein, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Humans
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - *Colorectal Neoplasms/pathology
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - *MicroRNAs/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - RNA, Messenger
MH  - Tumor Suppressor Proteins/metabolism
MH  - Animals
OTO - NOTNLM
OT  - Colorectal cancer
OT  - HNRNPC
OT  - MiR-33a-5p
OT  - RASSF8
OT  - RASSF8-AS1
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/07/16 06:00
MHDA- 2022/10/26 06:00
CRDT- 2022/07/15 18:22
PHST- 2021/12/02 00:00 [received]
PHST- 2022/06/10 00:00 [revised]
PHST- 2022/06/26 00:00 [accepted]
PHST- 2022/07/16 06:00 [pubmed]
PHST- 2022/10/26 06:00 [medline]
PHST- 2022/07/15 18:22 [entrez]
AID - S0344-0338(22)00240-0 [pii]
AID - 10.1016/j.prp.2022.153996 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2022 Sep;237:153996. doi: 10.1016/j.prp.2022.153996. Epub 2022 
      Jun 30.