PMID- 35840654
OWN - NLM
STAT- MEDLINE
DCOM- 20220719
LR  - 20220922
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Jul 15
TI  - Construction of a T7 phage display nanobody library for bio-panning and 
      identification of chicken dendritic cell-specific binding nanobodies.
PG  - 12122
LID - 10.1038/s41598-022-16378-x [doi]
LID - 12122
AB  - Dendritic cells (DCs) are the antigen-presenting cells that initiate and direct 
      adaptive immune responses, and thus are critically important in vaccine design. 
      Although DC-targeting vaccines have attracted attention, relevant studies on 
      chicken are rare. A high diversity T7 phage display nanobody library was 
      constructed for bio-panning of intact chicken bone marrow DCs to find DC-specific 
      binding nanobodies. After three rounds of screening, 46 unique sequence phage 
      clones were identified from 125 randomly selected phage clones. Several 
      DC-binding phage clones were selected using the specificity assay. Phage-54, -74, 
      -16 and -121 bound not only with chicken DCs, but also with duck and goose DCs. 
      In vitro, confocal microscopy observation demonstrated that phage-54 and phage-74 
      efficiently adsorbed onto DCs within 15 min compared to T7-wt. The pull-down 
      assay, however, did not detect any of the previously reported proteins for 
      chicken DCs that could have interacted with the nanobodies displayed on phage-54 
      and phage-74. Nonetheless, Specified pathogen-free chickens immunized with 
      phage-54 and phage-74 displayed higher levels of anti-p10 antibody than the 
      T7-wt, indicating enhanced antibody production by nanobody mediated-DC targeting. 
      Therefore, this study identified two avian (chicken, duck and goose) DC-specific 
      binding nanobodies, which may be used for the development of DC-targeting 
      vaccines.
CI  - (c) 2022. The Author(s).
FAU - Xu, Hai
AU  - Xu H
AD  - Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary 
      Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, 
      225300, Jiangsu Province, People's Republic of China.
AD  - Discipline: Microbiology, School of Life Sciences, College of Agriculture, 
      Engineering and Science, University of KwaZulu-Natal, Durban, 4000, South Africa.
AD  - Institute of Veterinary Immunology and Engineering, Jiangsu Academy of 
      Agricultural Science, Nanjing, 210014, Jiangsu Province, People's Republic of 
      China.
FAU - Li, Ling
AU  - Li L
AD  - Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary 
      Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, 
      225300, Jiangsu Province, People's Republic of China.
FAU - Deng, Bihua
AU  - Deng B
AD  - Institute of Veterinary Immunology and Engineering, Jiangsu Academy of 
      Agricultural Science, Nanjing, 210014, Jiangsu Province, People's Republic of 
      China. dengbihua1981@163.com.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, 225009, People's Republic of China. 
      dengbihua1981@163.com.
FAU - Hong, Weiming
AU  - Hong W
AD  - Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary 
      Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, 
      225300, Jiangsu Province, People's Republic of China.
FAU - Li, Ruiting
AU  - Li R
AD  - Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary 
      Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, 
      225300, Jiangsu Province, People's Republic of China.
FAU - Guo, Zijie
AU  - Guo Z
AD  - Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary 
      Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, 
      225300, Jiangsu Province, People's Republic of China.
FAU - Hou, Jibo
AU  - Hou J
AD  - Institute of Veterinary Immunology and Engineering, Jiangsu Academy of 
      Agricultural Science, Nanjing, 210014, Jiangsu Province, People's Republic of 
      China.
FAU - Govinden, Roshini
AU  - Govinden R
AD  - Discipline: Microbiology, School of Life Sciences, College of Agriculture, 
      Engineering and Science, University of KwaZulu-Natal, Durban, 4000, South Africa.
FAU - Chenia, Hafizah Y
AU  - Chenia HY
AD  - Discipline: Microbiology, School of Life Sciences, College of Agriculture, 
      Engineering and Science, University of KwaZulu-Natal, Durban, 4000, South Africa. 
      cheniah@ukzn.ac.za.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220715
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Peptide Library)
RN  - 0 (Single-Domain Antibodies)
SB  - IM
MH  - Animals
MH  - Bacteriophage T7/genetics
MH  - *Bacteriophages/genetics
MH  - Chickens
MH  - Dendritic Cells
MH  - Peptide Library
MH  - *Single-Domain Antibodies
PMC - PMC9284966
COIS- The authors declare no competing interests.
EDAT- 2022/07/16 06:00
MHDA- 2022/07/20 06:00
PMCR- 2022/07/15
CRDT- 2022/07/15 23:22
PHST- 2022/02/17 00:00 [received]
PHST- 2022/07/08 00:00 [accepted]
PHST- 2022/07/15 23:22 [entrez]
PHST- 2022/07/16 06:00 [pubmed]
PHST- 2022/07/20 06:00 [medline]
PHST- 2022/07/15 00:00 [pmc-release]
AID - 10.1038/s41598-022-16378-x [pii]
AID - 16378 [pii]
AID - 10.1038/s41598-022-16378-x [doi]
PST - epublish
SO  - Sci Rep. 2022 Jul 15;12(1):12122. doi: 10.1038/s41598-022-16378-x.