PMID- 35841264
OWN - NLM
STAT- MEDLINE
DCOM- 20220908
LR  - 20220919
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 119
IP  - 10
DP  - 2022 Oct
TI  - Physiological suitability of sulfate-reducing granules for the development of 
      bioconcrete.
PG  - 2743-2756
LID - 10.1002/bit.28184 [doi]
AB  - Regular monitoring and timely repair of concrete cracks are required to minimize 
      further deterioration. Self-healing of cracks has been proposed as an alternative 
      to the crack maintenance procedures. One of the proposed techniques is to use 
      axenic cultures to exploit microbial-induced calcite precipitation (MICP). 
      However, such healing agents are not cost-effective for in situ use. As the 
      market for bio-based self-healing concrete necessitates a low-cost bio-agent, 
      nonaxenic sulfate reducing bacterial (SRB) granules were investigated in this 
      study through cultivation in an upflow anaerobic sludge blanket reactor. The 
      compact granules can protect the bacteria from adverse conditions without 
      encapsulation. This study investigated the microbial activities of SRB granules 
      at different temperatures, pH, and chemical oxygen demand concentrations which 
      the microbes would experience during the concrete casting and curing process. The 
      attenuation and recovery of microbial activities were measured before and after 
      the exposure. Moreover, the MICP yield was also tested for a possible use in 
      self-healing bioconcrete. The results consistently showed that SRB granules were 
      able to survive starvation, high temperature (50-60 degrees C), and high pH (12), 
      together with scanning electron microscope/energy dispersive spectrometry/X-ray 
      diffraction analysis evidence. Microbial staining analysis demonstrated the 
      formation of spores in the granules during their exposure to harsh conditions. 
      SRB granule was thus demonstrated to be a viable self-healing nonaxenic agent for 
      low-cost bioconcrete.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Chetty, Kirthi
AU  - Chetty K
AUID- ORCID: 0000-0002-8067-5424
AD  - School of Civil, Mining and Environmental Engineering, University of Wollongong, 
      Wollongong, New South Wales, Australia.
FAU - McCarthy, Timothy
AU  - McCarthy T
AD  - School of Civil, Mining and Environmental Engineering, University of Wollongong, 
      Wollongong, New South Wales, Australia.
FAU - Hai, Faisal
AU  - Hai F
AD  - School of Civil, Mining and Environmental Engineering, University of Wollongong, 
      Wollongong, New South Wales, Australia.
FAU - Zhang, Shuxin
AU  - Zhang S
AD  - School of Civil, Mining and Environmental Engineering, University of Wollongong, 
      Wollongong, New South Wales, Australia.
FAU - Song, Yarong
AU  - Song Y
AD  - Australian Centre for Water and Environmental Biotechnology, The University of 
      Queensland, St. Lucia, Queensland, Australia.
FAU - Jiang, Guangming
AU  - Jiang G
AD  - School of Civil, Mining and Environmental Engineering, University of Wollongong, 
      Wollongong, New South Wales, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220727
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Sewage)
RN  - 0 (Sulfates)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Bacteria
MH  - Calcium Carbonate
MH  - *Construction Materials/analysis/microbiology
MH  - Sewage/microbiology
MH  - *Sulfates
OTO - NOTNLM
OT  - bioconcrete
OT  - concrete
OT  - granular sludge
OT  - microbially induced calcite precipitation
OT  - self-healing
OT  - sporulation
OT  - sulfate reducing bacteria
EDAT- 2022/07/17 06:00
MHDA- 2022/09/09 06:00
CRDT- 2022/07/16 05:02
PHST- 2022/06/03 00:00 [revised]
PHST- 2022/03/18 00:00 [received]
PHST- 2022/07/10 00:00 [accepted]
PHST- 2022/07/17 06:00 [pubmed]
PHST- 2022/09/09 06:00 [medline]
PHST- 2022/07/16 05:02 [entrez]
AID - 10.1002/bit.28184 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2022 Oct;119(10):2743-2756. doi: 10.1002/bit.28184. Epub 2022 
      Jul 27.