PMID- 35841601
OWN - NLM
STAT- MEDLINE
DCOM- 20220811
LR  - 20220816
IS  - 1365-2761 (Electronic)
IS  - 0140-7775 (Linking)
VI  - 45
IP  - 9
DP  - 2022 Sep
TI  - The prevalence, antibiotic resistance and multilocus sequence typing of 
      colistin-resistant bacteria isolated from Penaeus vannamei farms in earthen ponds 
      and HDPE film-lined ponds in China.
PG  - 1289-1299
LID - 10.1111/jfd.13661 [doi]
AB  - The aquaculture environment, especially the culture ponds and aquaculture 
      products, is considered to be an important reservoir of colistin resistance 
      genes. However, systematic investigations of colistin resistance in Penaeus 
      vannamei farming in different culture modes are scarce. In this study, a total of 
      93 non-duplicated samples were collected from P. vannamei farms in five cities in 
      China from 2019 to 2021. The prevalence, antibiotic resistance and multilocus 
      sequence typing (MLST) of colistin-resistant bacteria were measured and analysed. 
      The results showed that among the 1601 isolates in P. vannamei and its 
      environmental samples, the pollution of colistin-resistant bacteria was serious 
      (the overall prevalence was 37.3% and 28.8%, respectively), regardless of the 
      earthen pond or high-density polyethylene (HDPE) film-lined pond. Among 533 
      isolates, the prevalence of mobile colistin resistance (mcr) genes, mcr-1, was 
      the highest (60%, 320/533), followed by mcr-4 (1.5%, 8/533), mcr-8 (0.9%, 5/533), 
      mcr-10 (0.6%, 3/533) and mcr-7 (0.4%, 2/533). The prevalence of mcr-1 in earthen 
      ponds was significantly higher than that in HDPE film-lined ponds (67.5% vs. 
      49.1%, p < .001). The dominant strain carrying mcr-1 was Bacillus spp. (54.1%, 
      173/320), followed by Enterobacter spp. (8.1%, 26/320), Staphylococcus spp. 
      (6.3%, 20/320) and Aeromonas spp. (5.3%, 17/320). The antibiotic resistance 
      profiles of 173 Bacillus spp. varied among different sampling locations and 
      culture types. These isolates were highly resistant to cefepime, ceftriaxone, 
      trimethoprim-sulfamethoxazole and ceftiofur (>45%), and multidrug-resistant 
      isolates were common (62.4%, 108/173). Sequence type (ST) 26 (37/66, 56%) was 
      found to be the most prevalent ST in mcr-1-positive Bacillus cereus isolated from 
      the aquaculture environment. In summary, our study pointed out that it is 
      necessary to continuously monitor antibiotic usage and its residues regardless of 
      the pond types, especially with regard to critical drugs such as colistin.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Que, Muyi
AU  - Que M
AD  - Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China.
FAU - Cao, Weiwei
AU  - Cao W
AD  - College of Food and Bioengineering, Guangdong Polytechnic of Science and Trade, 
      Guangzhou, China.
FAU - Zhang, Huang
AU  - Zhang H
AUID- ORCID: 0000-0003-1055-4667
AD  - Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China.
FAU - Shi, Lei
AU  - Shi L
AD  - Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China.
FAU - Ye, Lei
AU  - Ye L
AUID- ORCID: 0000-0003-1696-3701
AD  - Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China.
LA  - eng
GR  - 22008085/the National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20220716
PL  - England
TA  - J Fish Dis
JT  - Journal of fish diseases
JID - 9881188
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 9002-88-4 (Polyethylene)
RN  - Z67X93HJG1 (Colistin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacterial Proteins/genetics
MH  - China
MH  - Colistin/pharmacology
MH  - Drug Resistance, Bacterial/genetics
MH  - Microbial Sensitivity Tests
MH  - Multilocus Sequence Typing
MH  - *Penaeidae/microbiology
MH  - Plasmids
MH  - Polyethylene
MH  - Ponds
OTO - NOTNLM
OT  - Penaeus vannamei
OT  - MLST
OT  - colistin
OT  - multidrug resistance
OT  - prevalence
EDAT- 2022/07/17 06:00
MHDA- 2022/08/12 06:00
CRDT- 2022/07/16 13:52
PHST- 2022/05/12 00:00 [revised]
PHST- 2022/03/17 00:00 [received]
PHST- 2022/05/17 00:00 [accepted]
PHST- 2022/07/17 06:00 [pubmed]
PHST- 2022/08/12 06:00 [medline]
PHST- 2022/07/16 13:52 [entrez]
AID - 10.1111/jfd.13661 [doi]
PST - ppublish
SO  - J Fish Dis. 2022 Sep;45(9):1289-1299. doi: 10.1111/jfd.13661. Epub 2022 Jul 16.