PMID- 35842459
OWN - NLM
STAT- MEDLINE
DCOM- 20220719
LR  - 20220923
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Jul 16
TI  - Reduced dynamic loads due to hip dislocation induce acetabular cartilage 
      degeneration by IL-6 and MMP3 via the STAT3/periostin/NF-kappaB axis.
PG  - 12207
LID - 10.1038/s41598-022-16585-6 [doi]
LID - 12207
AB  - Developmental dysplasia of the hip (DDH) is characterized by anatomical 
      abnormalities of the hip joint, ranging from mild acetabular dysplasia to hip 
      subluxation and eventually dislocation. The mechanism underlying the cartilage 
      degeneration of the hip joints exposed to reduced dynamic loads due to hip 
      dislocation remains unknown. We established a rodent hip dislocation 
      (disarticulation; DA) model of DDH (DA-DDH rats and mice) by swaddling. 
      Expression levels of periostin (Postn) and catabolic factors, such as 
      interleukin-6 (IL-6) and matrix metalloproteinase 3 (Mmp3), increased and those 
      of chondrogenic markers decreased in the acetabular cartilage of the DA-DDH 
      models. Postn induced IL-6 and Mmp3 expression in chondrocytes through integrin 
      alphaVbeta3, focal adhesion kinase, Src, and nuclear factor-kappaB (NF-kappaB) signaling. The 
      microgravity environment created by a random positioning machine induced Postn 
      expression in chondrocytes through signal transducer and activator of 
      transcription 3 (STAT3) signaling. IL-6 stimulated Postn expression via STAT3 
      signaling. Furthermore, cartilage degeneration was suppressed in the acetabulum 
      of Postn(-/-) DA-DDH mice compared with that in the acetabulum of wild type 
      DA-DDH mice. In summary, reduced dynamic loads due to hip dislocation induced 
      acetabular cartilage degeneration via IL-6 and MMP3 through STAT3/periostin/NF-kappaB 
      signaling in the rodent DA-DDH models.
CI  - (c) 2022. The Author(s).
FAU - Nakamura, Yutaka
AU  - Nakamura Y
AD  - Department of Orthopaedic Surgery, Graduate School of Medicine, Gifu University, 
      1-1 Yanagido, Gifu, 501-1194, Japan.
FAU - Saitou, Mitsuru
AU  - Saitou M
AD  - Department of Orthopaedic Surgery, Graduate School of Medicine, Gifu University, 
      1-1 Yanagido, Gifu, 501-1194, Japan.
FAU - Komura, Shingo
AU  - Komura S
AD  - Department of Orthopaedic Surgery, Graduate School of Medicine, Gifu University, 
      1-1 Yanagido, Gifu, 501-1194, Japan.
FAU - Matsumoto, Kazu
AU  - Matsumoto K
AD  - Department of Orthopaedic Surgery, Graduate School of Medicine, Gifu University, 
      1-1 Yanagido, Gifu, 501-1194, Japan.
FAU - Ogawa, Hiroyasu
AU  - Ogawa H
AD  - Department of Orthopaedic Surgery, Graduate School of Medicine, Gifu University, 
      1-1 Yanagido, Gifu, 501-1194, Japan.
FAU - Miyagawa, Takaki
AU  - Miyagawa T
AD  - Department of Orthopaedic Surgery, Graduate School of Medicine, Gifu University, 
      1-1 Yanagido, Gifu, 501-1194, Japan.
FAU - Saitou, Takashi
AU  - Saitou T
AD  - Department of Molecular Medicine for Pathogenesis, Graduate School of Medicine, 
      Ehime University, Toon, Ehime, 791-0295, Japan.
FAU - Imamura, Takeshi
AU  - Imamura T
AD  - Department of Molecular Medicine for Pathogenesis, Graduate School of Medicine, 
      Ehime University, Toon, Ehime, 791-0295, Japan.
FAU - Imai, Yuuki
AU  - Imai Y
AD  - Division of Integrative Pathophysiology, Proteo-Science Center, Ehime University, 
      Toon, Ehime, 791-0295, Japan.
FAU - Takayanagi, Hiroshi
AU  - Takayanagi H
AD  - Department of Immunology, Graduate School of Medicine and Faculty of Medicine, 
      The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
FAU - Akiyama, Haruhiko
AU  - Akiyama H
AD  - Department of Orthopaedic Surgery, Graduate School of Medicine, Gifu University, 
      1-1 Yanagido, Gifu, 501-1194, Japan. hakiyama@gifu-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220716
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (STAT3 Transcription Factor)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.17 (Mmp3 protein, mouse)
SB  - IM
MH  - Acetabulum
MH  - Animals
MH  - Cartilage
MH  - *Cartilage Diseases
MH  - *Hip Dislocation
MH  - Interleukin-6
MH  - Matrix Metalloproteinase 3/genetics
MH  - Mice
MH  - NF-kappa B
MH  - Rats
MH  - STAT3 Transcription Factor
PMC - PMC9288549
COIS- The authors declare no competing interests.
EDAT- 2022/07/17 06:00
MHDA- 2022/07/20 06:00
PMCR- 2022/07/16
CRDT- 2022/07/16 23:17
PHST- 2022/04/12 00:00 [received]
PHST- 2022/07/12 00:00 [accepted]
PHST- 2022/07/16 23:17 [entrez]
PHST- 2022/07/17 06:00 [pubmed]
PHST- 2022/07/20 06:00 [medline]
PHST- 2022/07/16 00:00 [pmc-release]
AID - 10.1038/s41598-022-16585-6 [pii]
AID - 16585 [pii]
AID - 10.1038/s41598-022-16585-6 [doi]
PST - epublish
SO  - Sci Rep. 2022 Jul 16;12(1):12207. doi: 10.1038/s41598-022-16585-6.