PMID- 35844512
OWN - NLM
STAT- MEDLINE
DCOM- 20220719
LR  - 20220719
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 13
DP  - 2022
TI  - Identification of Key Pyroptosis-Related Genes and Distinct Pyroptosis-Related 
      Clusters in Periodontitis.
PG  - 862049
LID - 10.3389/fimmu.2022.862049 [doi]
LID - 862049
AB  - AIM: This study aims to identify pyroptosis-related genes (PRGs), their 
      functional immune characteristics, and distinct pyroptosis-related clusters in 
      periodontitis. METHODS: Differentially expressed (DE)-PRGs were determined by 
      merging the expression profiles of GSE10334, GSE16134, and PRGs obtained from 
      previous literatures and Molecular Signatures Database (MSigDB). Least absolute 
      shrinkage and selection operator (LASSO) regression was applied to screen the 
      prognostic PRGs and develop a prognostic model. Consensus clustering was applied 
      to determine the pyroptosis-related clusters. Functional analysis and 
      single-sample gene set enrichment analysis (ssGSEA) were performed to explore the 
      biological characteristics and immune activities of the clusters. The hub 
      pyroptosis-related modules were defined using weighted correlation network 
      analysis (WGCNA). RESULTS: Of the 26 periodontitis-related DE-PRGs, the highest 
      positive relevance was for High-Mobility Group Box 1 (HMGB1) and SR-Related CTD 
      Associated Factor 11 (SCAF11). A 14-PRG-based signature was developed through the 
      LASSO model. In addition, three pyroptosis-related clusters were obtained based 
      on the 14 prognostic PRGs. Caspase 3 (CASP3), Granzyme B (GZMB), Interleukin 1 
      Alpha (IL1A), IL1Beta (B), IL6, Phospholipase C Gamma 1 (PLCG1) and PYD And CARD 
      Domain Containing (PYCARD) were dysregulated in the three clusters. Distinct 
      biological functions and immune activities, including human leukocyte antigen 
      (HLA) gene expression, immune cell infiltration, and immune pathway activities, 
      were identified in the three pyroptosis-related clusters of periodontitis. 
      Furthermore, the pink module associated with endoplasmic stress-related functions 
      was found to be correlated with cluster 2 and was suggested as the hub 
      pyroptosis-related module. CONCLUSION: The study identified 14 key 
      pyroptosis-related genes, three distinct pyroptosis-related clusters, and one 
      pyroptosis-related gene module describing several molecular aspects of pyroptosis 
      in the pathogenesis and immune micro-environment regulation of periodontitis and 
      also highlighted functional heterogeneity in pyroptosis-related mechanisms.
CI  - Copyright (c) 2022 Ning, Acharya, Li, Schmalz and Huang.
FAU - Ning, Wanchen
AU  - Ning W
AD  - Stomatological Hospital, Southern Medical University, Guangzhou, China.
FAU - Acharya, Aneesha
AU  - Acharya A
AD  - Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, 
      India.
FAU - Li, Simin
AU  - Li S
AD  - Stomatological Hospital, Southern Medical University, Guangzhou, China.
FAU - Schmalz, Gerhard
AU  - Schmalz G
AD  - Department of Cariology, Endodontology and Periodontology, University Leipzig, 
      Leipzig, Germany.
FAU - Huang, Shaohong
AU  - Huang S
AD  - Stomatological Hospital, Southern Medical University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220629
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
SB  - IM
MH  - Gene Regulatory Networks
MH  - Humans
MH  - *Periodontitis/genetics
MH  - Prognosis
MH  - *Pyroptosis/genetics
PMC - PMC9281553
OTO - NOTNLM
OT  - immune microenvironment
OT  - inflammasome
OT  - periodontitis
OT  - prognostic
OT  - pyroptosis
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/19 06:00
MHDA- 2022/07/20 06:00
PMCR- 2022/01/01
CRDT- 2022/07/18 03:38
PHST- 2022/01/25 00:00 [received]
PHST- 2022/05/23 00:00 [accepted]
PHST- 2022/07/18 03:38 [entrez]
PHST- 2022/07/19 06:00 [pubmed]
PHST- 2022/07/20 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2022.862049 [doi]
PST - epublish
SO  - Front Immunol. 2022 Jun 29;13:862049. doi: 10.3389/fimmu.2022.862049. eCollection 
      2022.