PMID- 35845218
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220719
IS  - 2688-6146 (Electronic)
IS  - 2688-6146 (Linking)
VI  - 2
IP  - 4
DP  - 2021 Nov
TI  - Mechanism of increased efficacy of recombinant Fc-muTP-L309C compared to IVIg to 
      ameliorate mouse immune thrombocytopenia.
PG  - 789-793
LID - 10.1002/jha2.304 [doi]
AB  - Recombinant Fc-muTP-L309C is more efficacious than intravenous immunoglobulin 
      (IVIg) at ameliorating antibody-mediated autoimmune diseases through its effects 
      on Fcgamma receptors (FcgammaRs). Fc-muTP-L309C inhibited in-vitro FcgammaR-mediated 
      phagocytosis 10(4)/10(5)-fold better than IVIg. Fc-muTP-L309C, given 
      subcutaneously, recovered platelet counts in an immune thrombocytopenia (ITP) 
      mouse model to a higher degree than IVIg at a 10-fold lower dose. We show, using 
      confocal microscopy, that Fc-muTP-L309C binds to monocyte-macrophages and is 
      rapidly internalized, whereas, IVIg remains on the cell surface. Western blotting 
      showed that internalized FcgammaRIII is degraded through a lysosomal pathway, and 
      this reduction of cell surface FcgammaRIII is likely responsible for the increased 
      efficacy to ameliorate ITP.
CI  - (c) 2021 The Authors. eJHaem published by British Society for Haematology and John 
      Wiley & Sons Ltd.
FAU - Lewis, Bonnie J B
AU  - Lewis BJB
AD  - Donald R. Branch, BS, MT(ASCP)SBB, PhD Donald R. Branch, BS, MT(ASCP)SBB, PhD 30 
      Bond Street, Keenan Research Centre Toronto M5B 1W8 Canada.
AD  - Department of Laboratory Medicine and Pathobiology University of Toronto Toronto 
      Canada.
FAU - Binnington, Beth
AU  - Binnington B
AD  - Donald R. Branch, BS, MT(ASCP)SBB, PhD Donald R. Branch, BS, MT(ASCP)SBB, PhD 30 
      Bond Street, Keenan Research Centre Toronto M5B 1W8 Canada.
FAU - Blacquiere, Megan
AU  - Blacquiere M
AD  - Donald R. Branch, BS, MT(ASCP)SBB, PhD Donald R. Branch, BS, MT(ASCP)SBB, PhD 30 
      Bond Street, Keenan Research Centre Toronto M5B 1W8 Canada.
FAU - Spirig, Rolf
AU  - Spirig R
AD  - Research, CSL Biologics Research Center CSL Behring AG Wankdorfstrasse 10 Bern 
      3010 Switzerland.
FAU - Kasermann, Fabian
AU  - Kasermann F
AUID- ORCID: 0000-0002-4255-9350
AD  - Research, CSL Biologics Research Center CSL Behring AG Wankdorfstrasse 10 Bern 
      3010 Switzerland.
FAU - Branch, Donald R
AU  - Branch DR
AUID- ORCID: 0000-0002-8974-2451
AD  - Donald R. Branch, BS, MT(ASCP)SBB, PhD Donald R. Branch, BS, MT(ASCP)SBB, PhD 30 
      Bond Street, Keenan Research Centre Toronto M5B 1W8 Canada.
AD  - Department of Medicine University of Toronto Toronto Canada.
LA  - eng
PT  - Journal Article
DEP - 20210929
PL  - United States
TA  - EJHaem
JT  - EJHaem
JID - 101761942
PMC - PMC9175896
OTO - NOTNLM
OT  - ITP
OT  - ITP intravenous immunoglobulin
OT  - IVIG
OT  - IVIg
OT  - immune thrombocytopenia
OT  - immunotherapy
OT  - recombinant Fc hexamer
EDAT- 2022/07/19 06:00
MHDA- 2022/07/19 06:01
PMCR- 2021/09/29
CRDT- 2022/07/18 03:47
PHST- 2021/08/10 00:00 [received]
PHST- 2021/09/06 00:00 [revised]
PHST- 2021/09/09 00:00 [accepted]
PHST- 2022/07/18 03:47 [entrez]
PHST- 2022/07/19 06:00 [pubmed]
PHST- 2022/07/19 06:01 [medline]
PHST- 2021/09/29 00:00 [pmc-release]
AID - JHA2304 [pii]
AID - 10.1002/jha2.304 [doi]
PST - epublish
SO  - EJHaem. 2021 Sep 29;2(4):789-793. doi: 10.1002/jha2.304. eCollection 2021 Nov.