PMID- 35845923
OWN - NLM
STAT- MEDLINE
DCOM- 20220719
LR  - 20230630
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2022
DP  - 2022
TI  - Human Umbilical Cord Mesenchymal Stem Cells Improve Premature Ovarian Failure 
      through Cell Apoptosis of miR-100-5p/NOX4/NLRP3.
PG  - 3862122
LID - 10.1155/2022/3862122 [doi]
LID - 3862122
AB  - Premature ovarian failure refers to a series of symptoms of perimenopausal hot 
      flashes, night sweats, decreased libido, vaginal dryness, insomnia, reduced 
      menstruation, sparse hair, even amenorrhea, and even infertility before the age 
      of 40 due to the decline of ovarian function. Premature ovarian failure is a 
      common and difficult disease in gynecology. Its prevalence is increasing 
      gradually, and the trend is younger. The aim of this experiment was to elucidate 
      the role of human umbilical cord mesenchymal stem cells (HUCMSCs) in premature 
      ovarian failure and its mechanism. HUCMSCs, KGN cells, and HEK293T cells were 
      used in this experiment. Quantitative PCR and microarray analysis, ELISA 
      inflammation and oxidative stress kits, RNA pull-down assay, luciferase reporter 
      assay, proliferation assay, EDU staining, and Western blot analysis were used. In 
      an in vitro model of premature ovarian failure, HUCMSCs attenuated inflammatory 
      response, oxidative stress, and apoptosis. HUCMSCs ameliorated the premature 
      ovarian failure model. The miR-100-5p expression was induced by HUCMSCs through 
      methylation. miR-100-5p regulation influenced the role of HUCMSCs in an in vitro 
      model of premature ovarian failure. HUCMSCs inhibited the in vitro expression of 
      NOX4, NLRP3, and GSDMD proteins in the model. NOX4/NLRP3 signaling pathway 
      affects the role of HUCMSCs in an in vitro model of premature ovarian failure 
      through miR-100-5p. This experiment elucidated the role of HUCMSCs in premature 
      ovarian failure and its mechanism, with a view to providing a clinical reference.
CI  - Copyright (c) 2022 Jing Niu et al.
FAU - Niu, Jing
AU  - Niu J
AD  - Department of Gynecology, Guangdong Women and Children Hospital, 511400, China.
FAU - Yu, Fan
AU  - Yu F
AD  - Department of Gynecology, Guangdong Women and Children Hospital, 511400, China.
FAU - Luo, Xiping
AU  - Luo X
AD  - Department of Gynecology, Guangdong Women and Children Hospital, 511400, China.
FAU - Chen, Shiling
AU  - Chen S
AUID- ORCID: 0000-0001-6018-6087
AD  - Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical 
      University, Guangzhou 510000, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20220707
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (MIRN100 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - EC 1.6.3.- (NADPH Oxidase 4)
RN  - EC 1.6.3.- (NOX4 protein, human)
SB  - IM
RIN - Biomed Res Int. 2023 Jun 21;2023:9848903. PMID: 37388357
MH  - Apoptosis/genetics
MH  - Female
MH  - HEK293 Cells
MH  - Humans
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - NADPH Oxidase 4/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - *Primary Ovarian Insufficiency/genetics/therapy
MH  - Umbilical Cord/metabolism
PMC - PMC9283025
COIS- The authors declare that there are no conflicts of interest regarding the 
      publication of this paper.
EDAT- 2022/07/19 06:00
MHDA- 2022/07/20 06:00
PMCR- 2022/07/07
CRDT- 2022/07/18 03:56
PHST- 2022/05/11 00:00 [received]
PHST- 2022/06/02 00:00 [revised]
PHST- 2022/06/07 00:00 [accepted]
PHST- 2022/07/18 03:56 [entrez]
PHST- 2022/07/19 06:00 [pubmed]
PHST- 2022/07/20 06:00 [medline]
PHST- 2022/07/07 00:00 [pmc-release]
AID - 10.1155/2022/3862122 [doi]
PST - epublish
SO  - Biomed Res Int. 2022 Jul 7;2022:3862122. doi: 10.1155/2022/3862122. eCollection 
      2022.