PMID- 35846305
OWN - NLM
STAT- MEDLINE
DCOM- 20221227
LR  - 20221227
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 13
DP  - 2022
TI  - Genomic DNA Methylation in Diabetic Chronic Complications in Patients With Type 2 
      Diabetes Mellitus.
PG  - 896511
LID - 10.3389/fendo.2022.896511 [doi]
LID - 896511
AB  - AIM: To explore the relationship between genomic DNA methylation and diabetic 
      chronic complications. METHODS: 299 patients with type 2 diabetes mellitus (T2DM) 
      hospitalized in the Second Affiliated Hospital of Soochow University were 
      enrolled. We divided the patients into different complications groups and 
      corresponding non-complication groups. Clinical and biochemical parameters were 
      compared between the two groups. The level of genomic DNA methylation in 
      leukocytes was determined by high-performance liquid chromatography-tandem mass 
      spectrometry. RESULTS: (1) Age, duration of diabetes, creatinine (Cr), blood urea 
      nitrogen (BUN), genomic DNA methylation, 24- hour urine total protein (24-hUTP), 
      and intima-media thickness (IMT) were significantly higher in the carotid plaque 
      (CP) group. Waist-to-hip ratio (WHR), body mass index (BMI), estimated 
      glomerular- filtration rate (eGFR), and albumin (Alb) were significantly lower in 
      the CP group. Gender, age and BMI were the influencing factors of CP. (2) Age, 
      duration, Cr, BUN, urinary microalbumin creatinine ratio (UACR), systolic blood 
      pressure (SBP), TCSS, and 24- hUTP were significantly higher in the diabetic 
      retinopathy (DR) group. eGFR, 2h postprandial C- peptide, and Alb were lower in 
      the DR group. Age, duration, Cr, Alb, SBP, and the presence of DN were the 
      influencing factors of DR. (3) Age, duration, HbA1c, BUN, TCSS, SBP, and IMT(R) 
      were significantly higher in the diabetic nephropathy (DN) group. 2h postprandial 
      C-peptide, and Alb were lower in the DN group. HbA1c, BUN, DR, and HBP were the 
      influencing factors of DN. (4) Age, duration, total cholesterol (TC), low-density 
      lipoprotein (LDL-C), triglyceride (TG), Cr, BUN, uric acid (UA), and SBP were 
      significantly higher in the diabetic peripheral neuropathy (DPN) group. The level 
      of genomic DNA methylation and eGFR were significantly lower in the DPN group. 
      Age, duration, LDL-C, UA, the presence of DR, and the genomic DNA methylation 
      level were the influencing factors for DPN. Incorporating the level of genomic 
      DNA methylation into the prediction model could improve the ability to predict 
      DPN on the basis of conventional risk factors. CONCLUSION: Low level of genomic 
      DNA methylation is a relatively specific risk factor for DPN in patients with 
      T2DM and not a contributing factor to the other chronic complications.
CI  - Copyright (c) 2022 Wang, Yang, Zhu, Zhang, Jiang, Hu and Zhang.
FAU - Wang, Xixi
AU  - Wang X
AD  - Department of Endocrinology, The Second Affiliated Hospital, Soochow University, 
      Suzhou, China.
FAU - Yang, Wenhong
AU  - Yang W
AD  - Department of Nursing, The Second Affiliated Hospital, Soochow University, 
      Suzhou, China.
FAU - Zhu, Yunyan
AU  - Zhu Y
AD  - Department of Endocrinology, The Second Affiliated Hospital, Soochow University, 
      Suzhou, China.
FAU - Zhang, Shiyu
AU  - Zhang S
AD  - Department of Endocrinology, The Second Affiliated Hospital, Soochow University, 
      Suzhou, China.
FAU - Jiang, Miao
AU  - Jiang M
AD  - Department of Endocrinology, The Second Affiliated Hospital, Soochow University, 
      Suzhou, China.
FAU - Hu, Ji
AU  - Hu J
AD  - Department of Endocrinology, The Second Affiliated Hospital, Soochow University, 
      Suzhou, China.
FAU - Zhang, Hong-Hong
AU  - Zhang HH
AD  - Department of Endocrinology, The Second Affiliated Hospital, Soochow University, 
      Suzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220629
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
RN  - 0 (Cholesterol, LDL)
RN  - AYI8EX34EU (Creatinine)
RN  - 0 (Glycated Hemoglobin)
RN  - 268B43MJ25 (Uric Acid)
SB  - IM
MH  - Humans
MH  - Carotid Intima-Media Thickness
MH  - Cholesterol, LDL
MH  - Creatinine
MH  - *Diabetes Mellitus, Type 2/complications/genetics
MH  - *Diabetic Nephropathies
MH  - *Diabetic Neuropathies
MH  - *Diabetic Retinopathy/complications
MH  - DNA Methylation
MH  - Genomics
MH  - Glycated Hemoglobin
MH  - Uric Acid
PMC - PMC9277053
OTO - NOTNLM
OT  - LC-MS/MS
OT  - chronic complications
OT  - diabetic peripheral neuropathy
OT  - genomic DNA methylation
OT  - type 2 diabetes
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/19 06:00
MHDA- 2022/07/20 06:00
PMCR- 2022/01/01
CRDT- 2022/07/18 04:00
PHST- 2022/03/15 00:00 [received]
PHST- 2022/05/20 00:00 [accepted]
PHST- 2022/07/18 04:00 [entrez]
PHST- 2022/07/19 06:00 [pubmed]
PHST- 2022/07/20 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2022.896511 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2022 Jun 29;13:896511. doi: 
      10.3389/fendo.2022.896511. eCollection 2022.