PMID- 35846350
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220720
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 10
DP  - 2022
TI  - Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium 
      Indicator.
PG  - 880107
LID - 10.3389/fcell.2022.880107 [doi]
LID - 880107
AB  - Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming 
      powerful tools for neuroscience. Because of their spectral characteristics, the 
      use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, 
      light-scattering, and autofluorescence in mammalian tissues. In addition, NIR 
      GECIs do not suffer from cross-excitation artifacts when used with common 
      fluorescent indicators and optogenetics actuators. Although several NIR GECIs 
      have been developed, there is no NIR GECI currently available that would combine 
      the high brightness in cells and photostability with small size and fast response 
      kinetics. Here, we report a small FRET-based NIR fluorescent calcium indicator 
      iGECInano. We characterize iGECInano in vitro, in non-neuronal mammalian cells, 
      and primary mouse neurons. iGECInano demonstrates the improvement in the 
      signal-to-noise ratio and response kinetics compared to other NIR GECIs.
CI  - Copyright (c) 2022 Matlashov, Vera, Kasatkina, Khodakhah and Verkhusha.
FAU - Matlashov, Mikhail E
AU  - Matlashov ME
AD  - Department of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein 
      College of Medicine, Bronx, NY, United States.
FAU - Vera, Jorge
AU  - Vera J
AD  - Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, 
      United States.
FAU - Kasatkina, Ludmila A
AU  - Kasatkina LA
AD  - Department of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein 
      College of Medicine, Bronx, NY, United States.
FAU - Khodakhah, Kamran
AU  - Khodakhah K
AD  - Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, 
      United States.
FAU - Verkhusha, Vladislav V
AU  - Verkhusha VV
AD  - Department of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein 
      College of Medicine, Bronx, NY, United States.
AD  - Medicum, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
LA  - eng
GR  - R01 DA044761/DA/NIDA NIH HHS/United States
GR  - R01 MH115604/MH/NIMH NIH HHS/United States
PT  - Journal Article
DEP - 20220629
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC9277108
OTO - NOTNLM
OT  - FRET
OT  - GECI
OT  - biosensor
OT  - calcium imaging
OT  - far-red
OT  - iRFP
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/19 06:00
MHDA- 2022/07/19 06:01
PMCR- 2022/01/01
CRDT- 2022/07/18 04:01
PHST- 2022/02/21 00:00 [received]
PHST- 2022/05/27 00:00 [accepted]
PHST- 2022/07/18 04:01 [entrez]
PHST- 2022/07/19 06:00 [pubmed]
PHST- 2022/07/19 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 880107 [pii]
AID - 10.3389/fcell.2022.880107 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 10.3389/fcell.2022.880107. 
      eCollection 2022.