PMID- 35846883
OWN - NLM
STAT- MEDLINE
DCOM- 20230116
LR  - 20230116
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 10
DP  - 2022
TI  - Prevalence of HPV genotypes and assessment of their clinical relevance in 
      laryngeal squamous cell carcinoma in a northeastern state of Brazil-a 
      retrospective study.
PG  - e13684
LID - 10.7717/peerj.13684 [doi]
LID - e13684
AB  - BACKGROUND: A high prevalence and incidence of head and neck tumors make Brazil 
      the country with the third-highest number of cases of these malignant neoplasms. 
      The main risk factors are smoking and alcohol consumption; however, cases related 
      to the human papillomavirus (HPV) have tripled in number, demonstrating a 
      changing disease profile. Studies have reported the prevalence of HPV in 
      laryngeal squamous cell carcinoma (LSCC) to vary between 8% and 83%. The role of 
      HPV as an important causative factor in LSCC remains unclear. METHODS: This 
      retrospective study included 82 patients with LSCC diagnosed between 2014 and 
      2019 at two oncology hospitals in Sao Luis, Brazil. Sociodemographic and clinical 
      data, and the histopathologic characteristics of the tumors, were collected 
      directly from medical records. Genetic material was extracted from 
      paraffin-embedded samples using nested polymerase chain reaction (PCR) and 
      automated sequencing for HPV detection and genotyping. The results by social and 
      clinicopathologic variables were then compared using the chi-squared test and 
      multivariate analysis. RESULTS: Sociodemographic analysesdemonstrated that most 
      patients were men (87.8%), brown-skinned (75.6%), and resident in the state 
      capital (53.7%). They generally had a poor education status (53.7%), having only 
      an elementary school education (completed/incomplete), and 51.2% were 
      self-employed in occupations such as farming or fishing. Smoking and alcohol 
      consumption habits were observed in approximately half the patients. With respect 
      to clinical characteristics, 39% of patients exhibited T1/T2 staging, 51.2% had 
      no distant metastasis, and 30.5% had lymph node invasion. HPV DNA was detected in 
      half the samples (50%), with the high oncogenic type 16 being the most prevalent. 
      There was no significant relationship observed between the economic, educational, 
      occupational with the HPV LSCC in the presented data, although multivariate 
      analysis demonstrated that HPV DNA was more likely to be present in T3-T4 tumors 
      (p = 0.002).
CI  - (c)2022 Brito et al.
FAU - Brito, Charlles
AU  - Brito C
AD  - Programa de Pos-Graduacao em Saude do Adulto, Universidade Federal do Maranhao, 
      Sao Luis, Maranhao, Brazil.
FAU - Cossetti, Rachel D
AU  - Cossetti RD
AD  - Departamento de Medicina I, Universidade Federal do Maranhao, Sao Luis, Maranhao, 
      Brazil.
AD  - Departamento de Patologia, Instituto Maranhense de Oncologia Aldenora Belo, Sao 
      Luis, Maranhao, Brazil.
FAU - de Souza, Diego Agra
AU  - de Souza DA
AD  - Departamento de Patologia, Instituto Maranhense de Oncologia Aldenora Belo, Sao 
      Luis, Maranhao, Brazil.
FAU - Catanha, Marcos
AU  - Catanha M
AD  - Departamento de Patologia, Instituto Maranhense de Oncologia Aldenora Belo, Sao 
      Luis, Maranhao, Brazil.
FAU - de Matos Monteiro, Pablo
AU  - de Matos Monteiro P
AD  - Departamento de Farmacia, Universidade Federal do Maranhao, Sao Luis, Maranhao, 
      Brazil.
FAU - Vidal, Flavia Castello Branco
AU  - Vidal FCB
AD  - Programa de Pos-Graduacao em Saude do Adulto, Universidade Federal do Maranhao, 
      Sao Luis, Maranhao, Brazil.
AD  - Departamento de Morfologia, Universidade Federal do Maranhao, Sao Luis, Maranhao, 
      Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220712
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
MH  - Male
MH  - Humans
MH  - Female
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Retrospective Studies
MH  - *Laryngeal Neoplasms/epidemiology
MH  - *Papillomavirus Infections/epidemiology
MH  - Prevalence
MH  - Brazil/epidemiology
MH  - Clinical Relevance
MH  - *Carcinoma, Squamous Cell/epidemiology
MH  - *Head and Neck Neoplasms
MH  - Papillomaviridae/genetics
MH  - Human Papillomavirus Viruses
MH  - Genotype
PMC - PMC9285469
OTO - NOTNLM
OT  - Laryngeal neoplasms
OT  - Papillomavirus infections
OT  - Prevalence
OT  - Squamous cell carcinoma of head and neck
COIS- The authors declare there are no competing interests.
EDAT- 2022/07/19 06:00
MHDA- 2022/07/19 06:01
PMCR- 2022/07/12
CRDT- 2022/07/18 04:08
PHST- 2022/03/08 00:00 [received]
PHST- 2022/06/14 00:00 [accepted]
PHST- 2022/07/18 04:08 [entrez]
PHST- 2022/07/19 06:00 [pubmed]
PHST- 2022/07/19 06:01 [medline]
PHST- 2022/07/12 00:00 [pmc-release]
AID - 13684 [pii]
AID - 10.7717/peerj.13684 [doi]
PST - epublish
SO  - PeerJ. 2022 Jul 12;10:e13684. doi: 10.7717/peerj.13684. eCollection 2022.