PMID- 35847050
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220719
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 13
DP  - 2022
TI  - Baicalein Prevents Fructose-Induced Hepatic Steatosis in Rats: In the Regulation 
      of Fatty Acid De Novo Synthesis, Fatty Acid Elongation and Fatty Acid Oxidation.
PG  - 917329
LID - 10.3389/fphar.2022.917329 [doi]
LID - 917329
AB  - Non-alcoholic fatty liver disease (NAFLD), ranging from simple steatosis to 
      non-alcoholic steatohepatitis (NASH), hepatic fibrosis and even hepatocellular 
      carcinoma, is a liver disease worldwide without approved therapeutic drugs. 
      Baicalein (BAL), a flavonoid compound extracted from the Traditional Chinese 
      Medicine (TCM) Scutellariae Radix (Scutellaria baicalensis Georgi.), has been 
      used in TCM clinical practice for thousands of years to treat liver diseases due 
      to its "hepatoprotective effect". However, the underlying liver-protecting 
      mechanisms remain largely unknown. Here, we found that oral administration of BAL 
      significantly decreased excess serum levels of triglyceride (TG), low-density 
      lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST) as well as 
      hepatic TG in fructose-fed rats. Attenuation of the increased vacuolization and 
      Oil Red O staining area was evident on hepatic histological examination in 
      BAL-treated rats. Mechanistically, results of RNA-sequencing, western-blot, 
      real-time quantitative PCR (RT-qPCR) and hepatic metabolomics analyses indicated 
      that BAL decreased fructose-induced excessive nuclear expressions of mature 
      sterol regulatory element-binding protein 1c (mSREBP1c) and carbohydrate response 
      element-binding protein (ChREBP), which led to the decline of lipogenic molecules 
      [including fatty acid synthase (FASN), stearoyl-CoA desaturase 1 (SCD1), 
      elongation of very long chain fatty acids 6 (ELOVL6), acetyl-CoA carboxylase 
      (ACC)], accompanying with the alternation of hepatic fatty acids composition. 
      Meanwhile, BAL enhanced fatty acid oxidation by activating AMPK/PGC1alpha signaling 
      axis and PPARalpha signal pathway, which elicited high expression of carnitine 
      palmitoyl transferase 1alpha (CPT1alpha) and Acyl-CoA oxidase 1 (ACO1) in livers of 
      fructose-fed rats, respectively. BAL ameliorated fructose-induced hepatic 
      steatosis, which is associated with regulating fatty acid synthesis, elongation 
      and oxidation.
CI  - Copyright (c) 2022 Li, Zhang, Wang, Chen, Chen, Ke, Zuo and Wang.
FAU - Li, Pan
AU  - Li P
AD  - Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure 
      of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical 
      University, Chongqing, China.
FAU - Zhang, Ruoyu
AU  - Zhang R
AD  - Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure 
      of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical 
      University, Chongqing, China.
FAU - Wang, Meng
AU  - Wang M
AD  - Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure 
      of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical 
      University, Chongqing, China.
FAU - Chen, Yuwei
AU  - Chen Y
AD  - The Pharmacy Department, the Second People's Hospital of Jiulongpo District, 
      Chongqing, China.
FAU - Chen, Zhiwei
AU  - Chen Z
AD  - Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure 
      of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical 
      University, Chongqing, China.
FAU - Ke, Xiumei
AU  - Ke X
AD  - Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure 
      of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical 
      University, Chongqing, China.
FAU - Zuo, Ling
AU  - Zuo L
AD  - Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure 
      of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical 
      University, Chongqing, China.
FAU - Wang, Jianwei
AU  - Wang J
AD  - Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure 
      of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical 
      University, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20220630
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC9280198
OTO - NOTNLM
OT  - Baicalein
OT  - fatty acid elongation
OT  - fatty acid oxidation
OT  - fatty acid synthesis
OT  - hepatic steatosis
OT  - non-alcoholic fatty liver
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/19 06:00
MHDA- 2022/07/19 06:01
PMCR- 2022/06/30
CRDT- 2022/07/18 04:10
PHST- 2022/04/11 00:00 [received]
PHST- 2022/06/13 00:00 [accepted]
PHST- 2022/07/18 04:10 [entrez]
PHST- 2022/07/19 06:00 [pubmed]
PHST- 2022/07/19 06:01 [medline]
PHST- 2022/06/30 00:00 [pmc-release]
AID - 917329 [pii]
AID - 10.3389/fphar.2022.917329 [doi]
PST - epublish
SO  - Front Pharmacol. 2022 Jun 30;13:917329. doi: 10.3389/fphar.2022.917329. 
      eCollection 2022.