PMID- 35847582
OWN - NLM
STAT- MEDLINE
DCOM- 20220719
LR  - 20220719
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2022
DP  - 2022
TI  - Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying 
      circ_0050205 Attenuate Intervertebral Disc Degeneration.
PG  - 8983667
LID - 10.1155/2022/8983667 [doi]
LID - 8983667
AB  - OBJECTIVE: It has been reported that bone marrow mesenchymal stem cells (BMSCs) 
      are a potential source of autologous stem cells to support the nucleus pulposus 
      (NP) regeneration in intervertebral disc degeneration (IDD). Herein, we aim to 
      study the mechanism underlying the effects of BMSC-derived extracellular vesicles 
      (BMSC-EVs) on nucleus pulposus cells (NPCs) in IDD. METHODS: EVs were isolated 
      from BMSCs. An IDD model was surgically established in C57BL/6J mice. NPCs were 
      exposed to tBHP to establish an IDD cell model. RNA sequencing was performed to 
      identify differentially expressed circRNAs in NP tissues harvested from mice with 
      IDD. Interactions among circ_0050205, miR-665, and GPX4 were validated, and 
      different interventions were used to study the roles of these molecules in NPC 
      biological functions. RESULTS: BMSC-EVs promoted NPC survival and inhibited NPC 
      apoptosis and extracellular matrix (ECM) degradation. circ_0050205 expression was 
      downregulated in the NP tissues of IDD mice, and BMSC-EVs facilitated NPC 
      survival and suppressed ECM degradation in NPCs by transferring circ_0050205. 
      circ_0050205 sponged miR-665 and upregulated GPX4 expression. BMSC-EVs expressing 
      circ_0050205 promoted NPC survival-inhibited ECM degradation in NPCs and 
      alleviated IDD in mice via the miR-665/GPX4 axis. CONCLUSION: In conclusion, 
      BMSC-EVs promoted NPC survival-inhibited ECM degradation in NPCs and attenuated 
      IDD progression via the circ_0050205/miR-665/GPX4 axis.
CI  - Copyright (c) 2022 Xiao-Jun Yu et al.
FAU - Yu, Xiao-Jun
AU  - Yu XJ
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Liu, Qi-Kun
AU  - Liu QK
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Lu, Rui
AU  - Lu R
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Wang, Shan-Xi
AU  - Wang SX
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Xu, Hao-Ran
AU  - Xu HR
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Wang, Ying-Guang
AU  - Wang YG
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Bao, Yuan
AU  - Bao Y
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Jiang, Yong-Qiao
AU  - Jiang YQ
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Li, Meng-Wei
AU  - Li MW
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
FAU - Kang, Hao
AU  - Kang H
AUID- ORCID: 0000-0003-3670-9946
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430030, China.
LA  - eng
PT  - Journal Article
DEP - 20220705
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Extracellular Matrix/metabolism
MH  - *Extracellular Vesicles/metabolism
MH  - *Intervertebral Disc Degeneration/genetics/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *MicroRNAs/genetics/metabolism
PMC - PMC9277161
COIS- The authors declare that they have no competing interests.
EDAT- 2022/07/19 06:00
MHDA- 2022/07/20 06:00
PMCR- 2022/07/05
CRDT- 2022/07/18 04:18
PHST- 2021/10/09 00:00 [received]
PHST- 2022/04/13 00:00 [revised]
PHST- 2022/05/10 00:00 [accepted]
PHST- 2022/07/18 04:18 [entrez]
PHST- 2022/07/19 06:00 [pubmed]
PHST- 2022/07/20 06:00 [medline]
PHST- 2022/07/05 00:00 [pmc-release]
AID - 10.1155/2022/8983667 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2022 Jul 5;2022:8983667. doi: 10.1155/2022/8983667. 
      eCollection 2022.