PMID- 35847974
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220719
IS  - 2296-889X (Print)
IS  - 2296-889X (Electronic)
IS  - 2296-889X (Linking)
VI  - 9
DP  - 2022
TI  - Interpretation of the Genotype by Tissue Interactions of Four Genes (AFP1, CIRP, 
      YB-1, and HMGB1) in Takifugu rubripes Under Different Low-Temperature Conditions.
PG  - 897935
LID - 10.3389/fmolb.2022.897935 [doi]
LID - 897935
AB  - Background: The differential expression of the same gene in different tissues 
      could be due to the genotype effect, tissue effect, and/or genotype x tissue 
      interactions. However, the genetic mechanisms responsible for this differential 
      expression have not been reported to date. Methods: Four resistance genes to low 
      temperature, the genes for antifreeze protein (AFP), cold induced RNA binding 
      protein (CIRP), high mobility group protein box-1 (HMGB1), and Y-box binding 
      protein (YB-1), were measured by PCR in the liver, spleen, kidney, brain, heart, 
      intestine, muscle, gonad, and skin of Takifugu rubripes cultured under different 
      temperature conditions (18, 13, 8, and 5 degrees C). Split-split-plot analysis of 
      variance, additive main effects and multiplicative interaction (AMMI) and 
      genotype main effects and genotype x environment interaction (GGE) biplot 
      analysis were used to evaluate the effects of genotype x tissue interactions on 
      gene expression. Results: The results of split-split-plot analysis of variance 
      showed that water temperature has a significant effect on the expression of T. 
      rubripes cold resistant genes, while tissue x gene interaction has a highly 
      significant effect on it. AMMI analysis showed that the contributions of 
      genotype, tissue, and genotype x tissue interactions to the total variation in 
      gene expression followed two trends: 1) as temperature decreased, the gene effect 
      increased gradually and the genotype x tissue interaction decreased gradually; 2) 
      the gene effect at 18 and 13 degrees C was significantly lower than that at 8 and 5 degrees C, 
      while the interaction at 18 and 13 degrees C was significantly higher than that at 8 and 
      5 degrees C. GGE analysis showed that: at all temperatures except 8 degrees C, the expression 
      rankings of all four genes were highly positively correlated in all tissues 
      except muscle; the expression stability of the genes was the same at 18 degrees C/13 degrees C 
      and at 8 degrees C/5 degrees C; and AFP1 showed the best expression and stability among the four 
      genes. Conclusion: 8 degrees C/5 degrees C as the suitable temperature for such experiments for 
      T. rubripes. Among the four antifreeze genes evaluated in this study, AFP1 had 
      the best expression and stability.
CI  - Copyright (c) 2022 Wang, Liu and Ma.
FAU - Wang, Xinan
AU  - Wang X
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao, China.
AD  - Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for 
      Marine Science and Technology, Qingdao, China.
FAU - Liu, Zhifeng
AU  - Liu Z
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao, China.
AD  - Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for 
      Marine Science and Technology, Qingdao, China.
FAU - Ma, Aijun
AU  - Ma A
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Qingdao, China.
AD  - Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for 
      Marine Science and Technology, Qingdao, China.
LA  - eng
PT  - Journal Article
DEP - 20220630
PL  - Switzerland
TA  - Front Mol Biosci
JT  - Frontiers in molecular biosciences
JID - 101653173
PMC - PMC9280165
OTO - NOTNLM
OT  - Takifugu rubripes
OT  - genotype
OT  - interactions
OT  - resistance genes to low temperature
OT  - tissue
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/19 06:00
MHDA- 2022/07/19 06:01
PMCR- 2022/01/01
CRDT- 2022/07/18 04:23
PHST- 2022/03/16 00:00 [received]
PHST- 2022/06/08 00:00 [accepted]
PHST- 2022/07/18 04:23 [entrez]
PHST- 2022/07/19 06:00 [pubmed]
PHST- 2022/07/19 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 897935 [pii]
AID - 10.3389/fmolb.2022.897935 [doi]
PST - epublish
SO  - Front Mol Biosci. 2022 Jun 30;9:897935. doi: 10.3389/fmolb.2022.897935. 
      eCollection 2022.