PMID- 35850627
OWN - NLM
STAT- MEDLINE
DCOM- 20220721
LR  - 20220731
IS  - 1471-2253 (Electronic)
IS  - 1471-2253 (Linking)
VI  - 22
IP  - 1
DP  - 2022 Jul 18
TI  - Skin/muscle incision and retraction regulates the persistent postoperative pain 
      in rats by the Epac1/PKC-betaII pathway.
PG  - 230
LID - 10.1186/s12871-022-01771-w [doi]
LID - 230
AB  - Persistent postoperative pain causes influence the life quality of many patients. 
      The Epac/PKC pathway has been indicated to regulate mechanical hyperalgesia. The 
      present study used skin/muscle incision and retraction (SMIR) to induce 
      postoperative pain in rats and evaluated the Epac/PKC pathway in postoperative 
      pain. Mechanical allodynia was assessed by paw withdrawal threshold before and 
      after incision. The levels of Epac, PKC, proinflammatory cytokines, and 
      blood-nerve barrier-related proteins were assessed using Western blotting. We 
      found that SMIR induced the activation of the Epac/PKC pathway, mechanical 
      allodynia, and upregulation of Glut1, VEGF, and PGP9.5 proteins in dorsal root 
      ganglia. Under the influence of agonists of Epac/PKC, normal rats showed 
      mechanical allodynia and increased Glut1, VEGF, and PGP9.5 proteins. After 
      inhibition of Epac1 in rats with SMIR, mechanical allodynia was alleviated, and 
      proinflammatory cytokines and Glut1, VEGF, and PGP9.5 proteins were decreased. 
      Moreover, dorsal root ganglia neurons showed abnormal proliferation under the 
      activation of the Epac/PKC pathway. Using Captopril to protect vascular 
      endothelial cells after SMIR had a positive effect on postoperative pain. In 
      conclusion, SMIR regulates the persistent postoperative pain in rats by the 
      Epac/PKC pathway.
CI  - (c) 2022. The Author(s).
FAU - Qian, Jiashu
AU  - Qian J
AD  - Department of Anesthesiology, Taizhou Central Hospital (Affiliated Hospital of 
      Taizhou University), No. 999 Donghai Avenue, Jiaojiang Economic Development Zone, 
      Taizhou City, 318000, Zhejiang Province, China.
FAU - Lin, Xuezheng
AU  - Lin X
AD  - Department of Anesthesiology, Taizhou Central Hospital (Affiliated Hospital of 
      Taizhou University), No. 999 Donghai Avenue, Jiaojiang Economic Development Zone, 
      Taizhou City, 318000, Zhejiang Province, China.
FAU - Zhou, Zhili
AU  - Zhou Z
AD  - Department of Anesthesiology, Taizhou Central Hospital (Affiliated Hospital of 
      Taizhou University), No. 999 Donghai Avenue, Jiaojiang Economic Development Zone, 
      Taizhou City, 318000, Zhejiang Province, China. Zhouzhl@tzzxyy.com.
LA  - eng
PT  - Journal Article
DEP - 20220718
PL  - England
TA  - BMC Anesthesiol
JT  - BMC anesthesiology
JID - 100968535
RN  - 0 (Cytokines)
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Rapgef3 protein, rat)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 2.7.11.13 (Protein Kinase C beta)
SB  - IM
MH  - Animals
MH  - Cytokines/metabolism
MH  - Endothelial Cells/metabolism
MH  - Ganglia, Spinal/metabolism
MH  - Glucose Transporter Type 1
MH  - Guanine Nucleotide Exchange Factors/*metabolism
MH  - *Hyperalgesia/complications
MH  - Muscles/metabolism
MH  - Pain, Postoperative/etiology
MH  - Protein Kinase C beta/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Skin
MH  - *Vascular Endothelial Growth Factor A
PMC - PMC9290233
OTO - NOTNLM
OT  - Epac1/PKC-betaII
OT  - Persistent postoperative pain
OT  - Skin/muscle incision and retraction
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/07/20 06:00
MHDA- 2022/07/22 06:00
PMCR- 2022/07/18
CRDT- 2022/07/19 07:37
PHST- 2022/01/07 00:00 [received]
PHST- 2022/07/11 00:00 [accepted]
PHST- 2022/07/19 07:37 [entrez]
PHST- 2022/07/20 06:00 [pubmed]
PHST- 2022/07/22 06:00 [medline]
PHST- 2022/07/18 00:00 [pmc-release]
AID - 10.1186/s12871-022-01771-w [pii]
AID - 1771 [pii]
AID - 10.1186/s12871-022-01771-w [doi]
PST - epublish
SO  - BMC Anesthesiol. 2022 Jul 18;22(1):230. doi: 10.1186/s12871-022-01771-w.