PMID- 35850813
OWN - NLM
STAT- MEDLINE
DCOM- 20220720
LR  - 20220720
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 47
IP  - 13
DP  - 2022 Jul
TI  - [Regulation of vascular endothelial cell activator expression by tanshinone Ⅱ_A 
      based on PI3K/Akt signaling pathway].
PG  - 3589-3596
LID - 10.19540/j.cnki.cjcmm.20211129.703 [doi]
AB  - The present study investigated the regulatory effect of tanshinone Ⅱ_A(TAⅡ_A) on 
      activator expression in human umbilical vein endothelial cells(HUVECs) and the 
      effect on the phosphoinositide 3-kinase(PI3 K)/protein kinase B(Akt) signaling 
      pathway in patients with antiphospholipid syndrome(APS). HUVECs cultured in vitro 
      were divided into a medium group, a blank control group, an APS model group, an 
      APS+LY5 group, an APS+LY10 group, an APS+LY20 group, an APS+TAⅡ_A5 group, an 
      APS+TAⅡ_A10 group, an APS+TAⅡ_A20 group, and an APS+TAⅡ_A10+LY10 group. The 
      effects of LY294002 and TAⅡ_A at different concentrations on the secretion of 
      interleukin-6(IL-6), interleukin-8(IL-8), and monocyte chemoattractant 
      protein-1(MCP-1) by HUVECs were investigated. The effects on the mRNA expression 
      of annexin A2(ANXA2), PI3 K, Akt, and E-cadherin(E-cad) were detected by 
      quantitative polymerase chain reaction(qPCR), and Western blot was used to 
      determine the effects on the protein expression of ANXA2, p-PI3 K/PI3 K, 
      p-Akt/Akt, and E-cad. The results revealed that compared with the APS model 
      group, the APS+TAⅡ_A10 group showed statistically reduced IL-6 and MCP-1 and 
      increased IL-8 in a concentration-dependent manner with the increase in TAⅡ_A 
      dose, while the APS+TAⅡ_A10 group showed increased mRNA and protein expression of 
      ANXA2, PI3 K, Akt, and E-cad(P<0.05 or P<0.01) in a concentration-dependent 
      manner with the increase in TAⅡ_A dose. The findings indicated that the serum of 
      APS patients could lead to the decreased mRNA and protein expression levels of 
      ANXA2, PI3 K, Akt, and E-cad in HUVECs, increased secretion of IL-6 and MCP-1, 
      and reduced secretion of IL-8, and activate vascular endothelial cells. In 
      contrast, once the PI3 K/Akt signaling pathway was blocked, the mRNA and protein 
      expression of ANXA2 and E-cad significantly decreased, IL-6 and MCP-1 secretion 
      significantly increased, and IL-8 secretion was significantly reduced. It 
      suggests that TAⅡ_A regulates the activation of vascular endothelial cells in APS 
      patients by activating the PI3 K/Akt signaling pathway.
FAU - Tong, Xing-Li
AU  - Tong XL
AD  - Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 
      210004,China.
FAU - Tan, Yong
AU  - Tan Y
AD  - Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 
      210004,China.
FAU - Yin, Yan-Yun
AU  - Yin YY
AD  - Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 
      210004,China.
FAU - Guo, Yin-Hua
AU  - Guo YH
AD  - Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 
      210004,China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Abietanes)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 03UUH3J385 (tanshinone)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Abietanes
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Interleukin-6/genetics/metabolism
MH  - Interleukin-8/metabolism
MH  - *Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - *Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - PI3K/Akt
OT  - antiphospholipid syndrome
OT  - tanshinone Ⅱ_A
OT  - vascular endothelial activation
EDAT- 2022/07/20 06:00
MHDA- 2022/07/22 06:00
CRDT- 2022/07/19 07:49
PHST- 2022/07/19 07:49 [entrez]
PHST- 2022/07/20 06:00 [pubmed]
PHST- 2022/07/22 06:00 [medline]
AID - 10.19540/j.cnki.cjcmm.20211129.703 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2022 Jul;47(13):3589-3596. doi: 
      10.19540/j.cnki.cjcmm.20211129.703.