PMID- 35851124
OWN - NLM
STAT- MEDLINE
DCOM- 20221228
LR  - 20221228
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 47
IP  - 12
DP  - 2022 Jun
TI  - [Effect of Linggui Zhugan Decoction-containing serum on mitochondrial oxidative 
      stress in cardiomyocytes: an exploration based on Nrf2/BNIP3 signaling pathway].
PG  - 3303-3311
LID - 10.19540/j.cnki.cjcmm.20220111.401 [doi]
AB  - This study aimed to explore the effect of Linggui Zhugan 
      Decoction(LGZGD)-containing serum on mitochondrial oxidative stress in 
      cardiomyocytes based on the NF-E2-related factor2(Nrf2)/Bcl-2/adenovirus E1 B 19 
      kDa interacting protein(BNIP3) signaling pathway. After the preparation of 
      LGZGD-containing serum and blank serum, H9 c2 cardiomyocytes were exposed to 
      H_2O_2 for inducing oxidative stress in vitro. The H9 c2 cells were divided into 
      four groups, namely normal control group, H_2O_2 model group, 20% blank serum 
      group, and 20% LGZGD-containing serum group. After the cells were pre-treated 
      with different types of serum for 12 h and cultured with 100 mumol.L~(-1 )H_2O_2 
      for 6 h, the reactive oxygen species(ROS) level in each group was detected by 
      DCFH-DA, and the openness of mitochondrial permeability transition pore(mPTP) was 
      measured using a calcein AM fluorescent probe. The expression levels of 
      cytoplasmic cytochrome C(CytC), mitochondrial CytC, cytoplasmic and nuclear Nrf2, 
      and BNIP3 were detected by Western blot. Nrf2-silenced H9 c2 cells were prepared 
      by transfecting them with siRNA-Nrf2 for investigating the efficacy of 
      LGZGD-containing serum in regulating ROS, mPTP, cytoplasmic and mitochondrial 
      CytC, and BNIP3. The results showed that compared with the normal control group, 
      H_2O_2 significantly increased the ROS content and mPTP openness(P<0.01), and 
      the expression of Nrf2, BNIP3, and cytoplasmic CytC(P<0.01), and decreased the 
      expression of mitochondrial CytC(P<0.01), without causing obvious change in 
      cytoplasmic Nrf2. LGZGD-containing serum significantly lowered ROS 
      content(P<0.01), inhibited mPTP openness(P<0.01), down-regulated the 
      expression of cytoplasmic CytC and BNIP3(P<0.01), up-regulated mitochondrial 
      CytC expression(P<0.01), and promoted Nrf2 nuclear translocation(P<0.05). 
      However, after Nrf2 silencing, the reduced ROS production, diminished BNIP3 
      expression, and inhibited mPTP openness and CytC release induced by 
      LGZGD-containing serum were reversed(P<0.01). These results have suggested 
      that LGZGD-containing serum is able to alleviate the mitochondrial oxidative 
      stress injury of cardiomyocytes by regulating the Nrf2/BNIP3 signaling pathway.
FAU - Tang, Tong-Juan
AU  - Tang TJ
AD  - Graduate School of Anhui University of Chinese Medicine Hefei 230031, China.
FAU - Wang, Xiang
AU  - Wang X
AD  - Graduate School of Anhui University of Chinese Medicine Hefei 230031, China.
FAU - Zhou, Peng
AU  - Zhou P
AD  - Graduate School of Anhui University of Chinese Medicine Hefei 230031, China.
FAU - Wang, Liang
AU  - Wang L
AD  - Graduate School of Anhui University of Chinese Medicine Hefei 230031, China.
FAU - Shi, Hui
AU  - Shi H
AD  - Graduate School of Anhui University of Chinese Medicine Hefei 230031, China.
FAU - Huang, Jin-Ling
AU  - Huang JL
AD  - Graduate School of Anhui University of Chinese Medicine Hefei 230031, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Mitochondrial Permeability Transition Pore)
SB  - IM
MH  - Apoptosis
MH  - *Myocytes, Cardiac
MH  - *NF-E2-Related Factor 2/genetics/metabolism
MH  - Oxidative Stress
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction
MH  - Mitochondrial Permeability Transition Pore
OTO - NOTNLM
OT  - Linggui Zhugan Decoction(LGZGD)
OT  - apoptosis
OT  - mitochondria
OT  - oxidative stress
OT  - reactive oxygen species(ROS)
EDAT- 2022/07/20 06:00
MHDA- 2022/07/22 06:00
CRDT- 2022/07/19 08:07
PHST- 2022/07/19 08:07 [entrez]
PHST- 2022/07/20 06:00 [pubmed]
PHST- 2022/07/22 06:00 [medline]
AID - 10.19540/j.cnki.cjcmm.20220111.401 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2022 Jun;47(12):3303-3311. doi: 
      10.19540/j.cnki.cjcmm.20220111.401.