PMID- 35851126 OWN - NLM STAT- MEDLINE DCOM- 20220720 LR - 20220720 IS - 1001-5302 (Print) IS - 1001-5302 (Linking) VI - 47 IP - 12 DP - 2022 Jun TI - [Effect of Danlou Tablets on alleviating hepatic adipogenesis, inflammatory response, and insulin resistance in db/db mice]. PG - 3320-3327 LID - 10.19540/j.cnki.cjcmm.20211220.401 [doi] AB - This study explored the effect and potential mechanism of Danlou Tablets(DLT) on insulin resistance in db/db mice with type 2 diabetic mellitus(T2 DM). The db/db male mice were randomly assigned into model control(MC) group, metformin(MET, tablet, 100 mg.kg~(-1)) group, and DLT(1 g.kg~(-1)) group, and C57 BL/6 J mice were taken as normal control(NC) group. The mice in the MET group and DLT group were given corresponding drugs by gavage once a day for 16 weeks. The fasting blood glucose, glucose tolerance, and insulin tolerance were measured to evaluate the effect of DLT on blood glucose and insulin resistance in diabetic mice. The serum free fatty acid, triacylglycerol, and total cholesterol levels were determined to evaluate the effect of DLT on blood lipids in diabetic mice. The liver index and perirenal fat index were calculated to measure the effect of DLT on lipid accumulation in non-adipose tissue and adipose tissue. Western blot was performed to determine the protein levels of insulin receptor-beta(IRbeta), phospho-IRbeta(p-IRbeta), phosphatidylinositol 3-kinase(PI3 K), and insulin receptor substrate-1(IRS-1) involved in IRS-1/PI3 K/Akt signaling pathway in the livers of mice to reveal the mechanism of DLT in alleviating insulin resistance in diabetic mice. The protein levels of sterol regulatory element binding protein-1(SREBP-1) and the mRNA levels of sterol regulatory element binding protein-1 c(SREBP-1 c), fatty acid synthase(FAS), acetyl-CoA carboxylase(ACC), diacylglycerol acyltransferase-1(Dgat1), and diacylglycerol acyltransferase-2(Dgat2) involved in the SREBP-1/FAS signaling pathway were detected to evaluate the effect of DLT on lipid metabolism in diabetic mice. Real-time quantitative PCR was employed to determine the mRNA levels of galectin-3(Gal-3), interleukin-6(IL-6), and monocyte chemoattractant protein-1(MCP-1) in mouse liver to evaluate the effect of DLT on inflammatory response in diabetic mice. The results showed that DLT significantly reduced the blood glucose and lipid levels and alleviated the insulin resistance in diabetic mice. Compared with the MC group, DLT significantly up-regulated the protein levels of p-IRbeta, PI3 K, and IRS-1(P<0.05 or P<0.01), and down-regulated the protein level of SREBP-1 in liver tissues of diabetic mice(P<0.05). DLT lowered the mRNA levels of SREBP-1 c, FAS, ACC, Dgat1, and Dgat2 related to lipid metabolism as well as those of Gal-3, IL-6, and MCP-1 associated with inflammation in the livers of diabetic mice(P<0.05 or P<0.01). The findings of this study suggest that DLT may alleviate insulin resistance in diabetic mice by regulating IRS-1/PI3 K/Akt signaling pathway and SREBP-1/FAS signaling pathway to reduce lipid production and inhibit inflammatory response. FAU - Pang, Ya-Fen AU - Pang YF AD - Tianjin University of Traditional Chinese Medicine Tianjin 301617, China. FAU - Huang, Ming AU - Huang M AD - Tianjin University of Traditional Chinese Medicine Tianjin 301617, China State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China. FAU - Li, Lin AU - Li L AD - Tianjin University of Traditional Chinese Medicine Tianjin 301617, China State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China. FAU - Zhao, Xin AU - Zhao X AD - Tianjin University of Traditional Chinese Medicine Tianjin 301617, China State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China. FAU - Zhang, Hong-Xia AU - Zhang HX AD - Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300250, China. FAU - Li, Yu-Hong AU - Li YH AD - Tianjin University of Traditional Chinese Medicine Tianjin 301617, China State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine Tianjin 301617, China. LA - chi PT - Journal Article PL - China TA - Zhongguo Zhong Yao Za Zhi JT - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JID - 8913656 RN - 0 (Blood Glucose) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Insulin) RN - 0 (Interleukin-6) RN - 0 (Lipids) RN - 0 (RNA, Messenger) RN - 0 (Sterol Regulatory Element Binding Protein 1) RN - 0 (danlou tablet) RN - EC 2.3.1.20 (Diacylglycerol O-Acyltransferase) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) SB - IM MH - Adipogenesis MH - Animals MH - Blood Glucose/metabolism MH - *Diabetes Mellitus, Experimental/metabolism MH - Diacylglycerol O-Acyltransferase/metabolism/pharmacology MH - Drugs, Chinese Herbal MH - Insulin/metabolism MH - *Insulin Resistance MH - Interleukin-6/metabolism MH - Lipids MH - Liver MH - Male MH - Mice MH - Proto-Oncogene Proteins c-akt/metabolism MH - RNA, Messenger/metabolism MH - Sterol Regulatory Element Binding Protein 1/genetics/metabolism OTO - NOTNLM OT - Danlou Tablets OT - IRS-1/PI3K/Akt OT - SREBP-1/FAS OT - inflammatory response OT - insulin resistance OT - type 2 diabetes mellitus EDAT- 2022/07/20 06:00 MHDA- 2022/07/22 06:00 CRDT- 2022/07/19 08:07 PHST- 2022/07/19 08:07 [entrez] PHST- 2022/07/20 06:00 [pubmed] PHST- 2022/07/22 06:00 [medline] AID - 10.19540/j.cnki.cjcmm.20211220.401 [doi] PST - ppublish SO - Zhongguo Zhong Yao Za Zhi. 2022 Jun;47(12):3320-3327. doi: 10.19540/j.cnki.cjcmm.20211220.401.