PMID- 35855981
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220721
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 8
IP  - 7
DP  - 2022 Jul
TI  - Microbially-Induced-Calcite-Precipitation (MICP): A biotechnological approach to 
      enhance the durability of concrete using Bacillus pasteurii and Bacillus 
      sphaericus.
PG  - e09879
LID - 10.1016/j.heliyon.2022.e09879 [doi]
LID - e09879
AB  - Developing bio-based self-healing concrete aims to minimize durability problems 
      related to cracking. In this study, MICP was used as a smart and eco-friendly 
      approach to produce bio-based durable materials. Bacillus pasteurii (BP) and 
      Bacillus sphaericus (BS) were added into mortar mixtures with 0.25% and 0.5% 
      cement weight. All treated samples exhibited a significant decline in water 
      uptake, capillary permeability, and volume of permeable voids, as compared to 
      control with no bacteria. All treated samples showed significant increase in 
      compressive strength by 28-50%, after 28 days of curing. At the age of 120 days, 
      the flexural strength of all treated samples was significantly increased by 
      19.29-65.94%. SEM imaging and EDAX confirmed that treated samples were denser 
      with less voids due to MICP. DTA verified that the calcite amount and the 
      crystallinity degree were improved in treated samples. Load deflection of 
      bacterial Reinforced-Laminates had less deformation than control. Reloaded 
      bacterial Reinforced-Laminates exhibited excellent restoration of 
      physico-mechanical properties and performance, after 28, 90, and 120 days, 
      confirming the healing process. Microbial self-healing is an innovative approach 
      for continuous repair of micro-cracks in concrete, improving its durability, thus 
      can reduce the maintenance costs.
CI  - (c) 2022 The Author(s).
FAU - Nasser, Amal A
AU  - Nasser AA
AD  - Department of Civil Engineering, Faculty of Engineering, Menoufia University, 
      Shebeen El-Kom, Egypt.
FAU - Sorour, Noha M
AU  - Sorour NM
AD  - Department of Industrial Biotechnology, Genetic Engineering and Biotechnology 
      Research Institute, University of Sadat City, Sadat City, Egypt.
FAU - Saafan, Mohamed A
AU  - Saafan MA
AD  - Department of Civil Engineering, Faculty of Engineering, Menoufia University, 
      Shebeen El-Kom, Egypt.
FAU - Abbas, Rateb N
AU  - Abbas RN
AD  - Department of Microbial Biotechnology, Genetic Engineering and Biotechnology 
      Research Institute, University of Sadat City, Sadat City, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20220705
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC9287183
OTO - NOTNLM
OT  - Bacillus pasteurii
OT  - Bacillus sphaericus
OT  - BioConcrete
OT  - Micro-cracks
OT  - Self-healing
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/21 06:00
MHDA- 2022/07/21 06:01
PMCR- 2022/07/05
CRDT- 2022/07/20 04:10
PHST- 2022/01/25 00:00 [received]
PHST- 2022/03/25 00:00 [revised]
PHST- 2022/06/30 00:00 [accepted]
PHST- 2022/07/20 04:10 [entrez]
PHST- 2022/07/21 06:00 [pubmed]
PHST- 2022/07/21 06:01 [medline]
PHST- 2022/07/05 00:00 [pmc-release]
AID - S2405-8440(22)01167-7 [pii]
AID - e09879 [pii]
AID - 10.1016/j.heliyon.2022.e09879 [doi]
PST - epublish
SO  - Heliyon. 2022 Jul 5;8(7):e09879. doi: 10.1016/j.heliyon.2022.e09879. eCollection 
      2022 Jul.