PMID- 35857144
OWN - NLM
STAT- MEDLINE
DCOM- 20221014
LR  - 20221014
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 167
IP  - 11
DP  - 2022 Nov
TI  - Role of zinc metalloprotease (ZMPSTE24) in porcine reproductive and respiratory 
      syndrome virus (PRRSV) replication in vitro.
PG  - 2281-2286
LID - 10.1007/s00705-022-05529-0 [doi]
AB  - The transmembrane zinc metalloprotease ZMPSTE24 works in cooperation with 
      interferon-induced transmembrane protein 3 (IFITM3) to restrict entry of several 
      enveloped viruses. We investigated the role of ZMPSTE24 in porcine reproductive 
      and respiratory syndrome virus (PRRSV) replication. ZMPSTE24 overexpression 
      significantly reduced PRRSV replication in MARC-145 cells. Interestingly, 
      knockdown of endogenous ZMPSTE24 did not significantly impact virus replication. 
      There was no significant difference in the percentage of PRRSV-positive cells and 
      viral RNA copies at 3 hours postinfection (hpi) between cells transfected with 
      ZMPSTE24-FLAG and the vector control. Our results suggest that ZMPSTE24 
      overexpression may restrict PRRSV replication at a post-entry step.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, 
      part of Springer Nature.
FAU - Katwal, Pratik
AU  - Katwal P
AD  - Department of Biology and Microbiology, South Dakota State University, Brookings, 
      SD, 57007, USA.
FAU - Aftab, Shamiq
AU  - Aftab S
AD  - Department of Biology and Microbiology, South Dakota State University, Brookings, 
      SD, 57007, USA.
FAU - Nelson, Eric
AU  - Nelson E
AD  - Department of Veterinary and Biomedical Sciences, South Dakota State University, 
      Brookings, SD, 57007, USA.
FAU - Hildreth, Michael
AU  - Hildreth M
AD  - Department of Biology and Microbiology, South Dakota State University, Brookings, 
      SD, 57007, USA.
FAU - Li, Shitao
AU  - Li S
AD  - Department of Microbiology and Immunology, Tulane University, New Orleans, LA, 
      70112, USA.
FAU - Wang, Xiuqing
AU  - Wang X
AUID- ORCID: 0000-0003-0491-4937
AD  - Department of Biology and Microbiology, South Dakota State University, Brookings, 
      SD, 57007, USA. Xiuqing.Wang@sdstate.edu.
LA  - eng
GR  - 2021-67016-34460/National Institute of Food and Agriculture/
GR  - SD00H660-19/National Institute of Food and Agriculture/
GR  - SD00R656-16/National Institute of Food and Agriculture/
PT  - Journal Article
DEP - 20220720
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (RNA, Viral)
RN  - 9008-11-1 (Interferons)
RN  - EC 3.4.- (Metalloproteases)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Interferons
MH  - Metalloproteases/genetics
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - RNA, Viral
MH  - Swine
MH  - Virus Replication/genetics
MH  - Zinc
EDAT- 2022/07/21 06:00
MHDA- 2022/10/15 06:00
CRDT- 2022/07/20 11:18
PHST- 2022/02/03 00:00 [received]
PHST- 2022/05/23 00:00 [accepted]
PHST- 2022/07/21 06:00 [pubmed]
PHST- 2022/10/15 06:00 [medline]
PHST- 2022/07/20 11:18 [entrez]
AID - 10.1007/s00705-022-05529-0 [pii]
AID - 10.1007/s00705-022-05529-0 [doi]
PST - ppublish
SO  - Arch Virol. 2022 Nov;167(11):2281-2286. doi: 10.1007/s00705-022-05529-0. Epub 
      2022 Jul 20.