PMID- 35858290
OWN - NLM
STAT- MEDLINE
DCOM- 20220803
LR  - 20220826
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 56
IP  - 15
DP  - 2022 Aug 2
TI  - Microdroplet-Based In Situ Characterization Of The Dynamic Evolution Of Amorphous 
      Calcium Carbonate during Microbially Induced Calcium Carbonate Precipitation.
PG  - 11017-11026
LID - 10.1021/acs.est.1c08858 [doi]
AB  - Amorphous calcium carbonate (ACC) plays an important role in microbially induced 
      calcium carbonate precipitation (MICP), which has great potential in broad 
      applications such as building restoration, CO(2) sequestration, and 
      bioremediation of heavy metals, etc. However, our understanding of ACC is still 
      limited. By combining microscopy of cell-laden microdroplets with confocal Raman 
      microspectroscopy, we investigated the ACC dynamics during MICP. The results show 
      that MICP inside droplets can be divided into three stages: liquid, gel-like ACC, 
      and precipitated CaCO(3) stages. In the liquid stage, the droplets are 
      transparent. As the MICP process continues into the gel-like stage, the ACC 
      structure appears and the droplets become opaque. Subsequently, dissolution of 
      the gel-like structure is accompanied by growth of precipitated CaCO(3) crystals. 
      The size, morphology, and lifetime of the gel-like structures depend on the 
      Ca(2+) concentration. Using polystyrene colloids as tracers, we find that the 
      colloids exhibit diffusive behavior in both the liquid and precipitated CaCO(3) 
      stages, while their motion becomes arrested in the gel-like ACC stage. These 
      results provide direct evidence for the formation-dissolution process of the 
      ACC-formed structure and its gel-like mechanical properties. Our work provides a 
      detailed view of the time evolution of ACC and its mechanical properties at the 
      microscale level, which has been lacking in previous studies.
FAU - Feng, Chunying
AU  - Feng C
AD  - Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems 
      Bioengineering (Ministry of Education), School of Chemical Engineering and 
      Technology, Tianjin University, Tianjin 300072, P.R. China.
FAU - Zhao, Shufeng
AU  - Zhao S
AD  - School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 
      305-8577, Japan.
FAU - Zong, Yiwu
AU  - Zong Y
AUID- ORCID: 0000-0003-0326-4581
AD  - Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems 
      Bioengineering (Ministry of Education), School of Chemical Engineering and 
      Technology, Tianjin University, Tianjin 300072, P.R. China.
FAU - He, Qing
AU  - He Q
AD  - School of Chemical Engineering and Technology, Tianjin University, Tianjin, 
      300072, P. R. China.
FAU - Winarto, William
AU  - Winarto W
AD  - School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 
      305-8577, Japan.
FAU - Zhang, Wenchao
AU  - Zhang W
AD  - School of Chemistry and Life Sciences, Suzhou University of Science and 
      Technology, Suzhou 215009, P.R. China.
FAU - Utada, Andrew S
AU  - Utada AS
AUID- ORCID: 0000-0003-4542-6315
AD  - Faculty of Life and Environmental Sciences and Microbial Institute for 
      Sustainability (MiCS), University of Tsukuba, Ibaraki 305-8577, Japan.
FAU - Zhao, Kun
AU  - Zhao K
AUID- ORCID: 0000-0003-3928-1981
AD  - Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems 
      Bioengineering (Ministry of Education), School of Chemical Engineering and 
      Technology, Tianjin University, Tianjin 300072, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220720
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Calcium Carbonate/chemistry
MH  - Chemical Precipitation
MH  - *Sporosarcina
OTO - NOTNLM
OT  - Raman microspectroscopy
OT  - Sporosarcina pasteurii
OT  - amorphous calcium carbonate (ACC)
OT  - microbially induced calcium carbonate precipitation (MICP)
OT  - microdroplets
EDAT- 2022/07/21 06:00
MHDA- 2022/08/04 06:00
CRDT- 2022/07/20 17:02
PHST- 2022/07/21 06:00 [pubmed]
PHST- 2022/08/04 06:00 [medline]
PHST- 2022/07/20 17:02 [entrez]
AID - 10.1021/acs.est.1c08858 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2022 Aug 2;56(15):11017-11026. doi: 10.1021/acs.est.1c08858. 
      Epub 2022 Jul 20.