PMID- 35859377
OWN - NLM
STAT- MEDLINE
DCOM- 20220722
LR  - 20220722
IS  - 1003-0034 (Print)
IS  - 1003-0034 (Linking)
VI  - 35
IP  - 7
DP  - 2022 Jul 25
TI  - [Correlation between osteopontin and cartilage oligomeric matrix protein levels 
      in joint synovial fluid of patients with knee osteoarthritis and disease 
      severity].
PG  - 655-60
LID - 10.12200/j.issn.1003-0034.2022.07.012 [doi]
AB  - OBJECTIVE: To investigate the correlation between osteopontin(OPN) and cartilage 
      oligomeric matrix protein (COMP) levels in synovial fluid of patients with knee 
      osteoarthritis(KOA) and the severity of the disease. METHODS: A total of 59 
      patients with KOA admitted to our hospital from February 2018 to May 2020 were 
      selected as the KOA group, including 25 males and 34 females, age ranged 60 to 75 
      years old with an average of(65.57+/-1.56) years old, the body mass index(BMI) 
      ranged 21.4 to 30.7(26.12+/-1.54) kg/m(2). After admission, X-ray examination was 
      performed, and Kellgren-Lawrence(K-L) grading system was used to evaluate the 
      X-ray examination results. There were 14 cases in gradeⅡ(K-L2 group), 27 cases in 
      grade Ⅲ(K-L3 group), and 18 cases in grade Ⅳ(K-L4 group). Eighteen patients who 
      underwent arthroscopy for ligament or meniscus disease without cartilage damage 
      were selected as control group, including 7 males and 11 females, age ranged 61 
      to 78 years old with an average of (64.88+/-1.60) years old, BMI ranged 22.8 to 
      29.9(25.89+/-1.49) kg/m(2). Before treatment, synovial fluid samples of subjects 
      were collected, and the OPN and COMP levels of synovial fluid were detected by 
      Elisa. The OPN and COMP levels of synovial fluid in KOA group and control group 
      were compared. The clinical data of KOA patients with different K-L grades were 
      collected, including gender, age and BMI. The biochemical indices of 
      interleukin-1 beta(IL-1beta), OPN, COMP and matrix metalloproteinase 3(MMP-3) in 
      synovitic fluid were detected by enzyme-linked immunoassay, and the clinical data 
      and biochemical indices of KOA patients with different K-L grades were compared. 
      Logistic regression was used to analyze the factors affecting the K-L 
      classification of KOA patients, and the area under the ROC curve(AUC) was used to 
      predict the severity of KOA. RESULTS: All the 59 patients were followed up for 8 
      to 27(15.75+/-3.27) months. The levels of OPN and COMP in synovial fluid in KOA 
      group were significantly higher than those in control group (t=16.991, 17.387, 
      P<0.001). The levels of IL-1beta, OPN, COMP and MMP-3 in synovitic fluid were 
      significantly different among those in different K-L grade KOA patients(P<0.001). 
      Compared with the K-L2 group, the levels of IL-1beta, OPN, COMP, and MMP-3 in the 
      synovial fluid of K-L3 and K-L4 were increased (P<0.05). Compared with the K-L3 
      group, the levels of IL-1beta, OPN, COMP, and MMP-3 in the K-L4 joint were increased 
      (P<0.05). Multivariate Logistic regression analysis showed that the levels of 
      OPN, COMP and MMP-3 were independent risk factors for K-L grading of KOA 
      patients(OR=6.653, 4.229, 1.579, P<0.001). AUC of OPN in synovial fluid 
      predicting K-L4 KOA was 0.720[95%CI(0.588-0.851)], and the sensitivity was 94.4%. 
      the specificity was 65.9%. The AUC of COMP in synoviac fluid predicting K-L4 KOA 
      was 0.731[95%CI(0.592-0.870)], the sensitivity was 88.9%, the specificity was 
      63.4%. The AUC of OPN combined with COMP in synoviac fluid predicting K-L4 KOA 
      was 0.839 [95%CI(0.724-0.953)], the sensitivity was 94.4%, and the specificity 
      was 51.2%. The AUC of OPN combined with COMP in synoviac fluid predicting K-L4 
      grade KOA was greater than that of OPN and COMP alone(Z=4.037, 3.540, P<0.05). 
      CONCLUSION: The levels of OPN and COMP in synovial fluid increase in patients 
      with KOA, and they increase with the increase of K-L grade. Synovitic fluid OPN 
      and COMP are independent risk factors affecting K-L grade of KOA patients, and 
      they have high AUC, sensitivity and specificity in predicting of K-L4 KOA, and 
      can be used to evaluate the progression of KOA disease.
FAU - Li, Xiao-Yun
AU  - Li XY
AD  - Department of Spine, Yueyang First People's Hospital, Yueyang 414000, Hunan, 
      China.
FAU - Shen, Zheng-Xiang
AU  - Shen ZX
AD  - Department of Spine, Yueyang First People's Hospital, Yueyang 414000, Hunan, 
      China.
FAU - Yang, Dan
AU  - Yang D
AD  - Department of Spine, Yueyang First People's Hospital, Yueyang 414000, Hunan, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Gu Shang
JT  - Zhongguo gu shang = China journal of orthopaedics and traumatology
JID - 9815790
RN  - 0 (Biomarkers)
RN  - 0 (Cartilage Oligomeric Matrix Protein)
RN  - 106441-73-0 (Osteopontin)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Aged
MH  - Biomarkers/metabolism
MH  - Cartilage Oligomeric Matrix Protein/metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Middle Aged
MH  - *Osteoarthritis, Knee/diagnosis
MH  - Osteopontin/metabolism
MH  - Severity of Illness Index
MH  - *Synovial Fluid/metabolism
OTO - NOTNLM
OT  - Cartilage oligomeric matrix protein
OT  - Osteoarthritis, knee
OT  - Osteopontin
OT  - Synovitic fluid
EDAT- 2022/07/22 06:00
MHDA- 2022/07/23 06:00
CRDT- 2022/07/21 01:42
PHST- 2022/07/21 01:42 [entrez]
PHST- 2022/07/22 06:00 [pubmed]
PHST- 2022/07/23 06:00 [medline]
AID - 10.12200/j.issn.1003-0034.2022.07.012 [doi]
PST - ppublish
SO  - Zhongguo Gu Shang. 2022 Jul 25;35(7):655-60. doi: 
      10.12200/j.issn.1003-0034.2022.07.012.