PMID- 35861275
OWN - NLM
STAT- MEDLINE
DCOM- 20220722
LR  - 20220727
IS  - 1743-2928 (Electronic)
IS  - 1351-0002 (Print)
IS  - 1351-0002 (Linking)
VI  - 27
IP  - 1
DP  - 2022 Dec
TI  - Beneficial role of naringin against methotrexate-induced injury to rat testes: 
      biochemical and ultrastructural analyses.
PG  - 158-166
LID - 10.1080/13510002.2022.2101832 [doi]
AB  - BACKGROUND: Methotrexate (MTX) is a commonly used chemotherapeutic drug that has 
      adverse toxic effects on germ cells. Naringin (NG) is a natural flavanone 
      glycoside, with different phytotherapeutic applications, and its possible 
      protective effects against MTX-induced testicular tissue damage were investigated 
      in this study. METHODS: Low and high doses of NG (40 and 80 mg/kg/day) were given 
      for 10 days by intraperitoneal (i.p.) injection and MTX (20 mg/kg i.p.) was given 
      at the 4th day of the experiment, with or without NG in rats. RESULTS: The 
      obtained results showed that exposure to MTX increased malondialdehyde (MDA) 
      levels and nitric oxide (NO) production compared with the control. In the 
      meantime, MTX depleted catalse (CAT), superoxide dismutase (SOD), glutathione 
      reductase (GR), glutathione peroxidase (GPx), and reduced glutathione (GSH) in 
      the testicular tissue. Further, serum testosterone levels were significantly 
      decreased in the MTX group. NG significantly counteracted the aforementioned 
      effects of MTX; however, NG80 was more effective in restoring SOD, GR, MDA and 
      NO. Interestingly, NG80 achieved a better improvement in the ultrastructural 
      pattern of the testicular cells in MTX-exposed rats. CONCLUSION: These results 
      indicated, for the first time, that NG could be a potential candidate therapy 
      against MTX-reprotoxic impacts.
FAU - Elsawy, Hany
AU  - Elsawy H
AUID- ORCID: 0000-0001-8250-4023
AD  - Department of Chemistry, College of Science, King Faisal University, Al-Ahsa, 
      Saudi Arabia.
AD  - Department of Chemistry, Faculty of Science, Tanta University, Tanta, Egypt.
FAU - Alzahrani, Abdullah M
AU  - Alzahrani AM
AD  - Department of Biological Sciences, College of Science, King Faisal University, 
      Al-Ahsa, Saudi Arabia.
FAU - Alfwuaires, Manal
AU  - Alfwuaires M
AD  - Department of Biological Sciences, College of Science, King Faisal University, 
      Al-Ahsa, Saudi Arabia.
FAU - Abdel-Moneim, Ashraf M
AU  - Abdel-Moneim AM
AD  - Department of Biological Sciences, College of Science, King Faisal University, 
      Al-Ahsa, Saudi Arabia.
AD  - Department of Zoology, Faculty of Science, Alexandria University, Alexandria, 
      Egypt.
FAU - Khalil, Mahmoud
AU  - Khalil M
AD  - Department of Zoology, Faculty of Science, Alexandria University, Alexandria, 
      Egypt.
AD  - Department of Biological Sciences, Faculty of Science, Beirut Arab University, 
      Beirut, Lebanon.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Redox Rep
JT  - Redox report : communications in free radical research
JID - 9511366
RN  - 0 (Antioxidants)
RN  - 0 (Flavanones)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - N7TD9J649B (naringin)
RN  - YL5FZ2Y5U1 (Methotrexate)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - *Flavanones/pharmacology/therapeutic use
MH  - Male
MH  - *Methotrexate/toxicity
MH  - Oxidative Stress
MH  - Rats
MH  - Rats, Wistar
MH  - Superoxide Dismutase/metabolism
MH  - Testis/metabolism
PMC - PMC9310850
OTO - NOTNLM
OT  - Methotrexate
OT  - antioxidants
OT  - naringin
OT  - nitric oxide
OT  - oxidative stress
OT  - testicular toxicity
OT  - testosterone
OT  - ultrastructure
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/07/22 06:00
MHDA- 2022/07/23 06:00
PMCR- 2022/07/21
CRDT- 2022/07/21 07:24
PHST- 2022/07/21 07:24 [entrez]
PHST- 2022/07/22 06:00 [pubmed]
PHST- 2022/07/23 06:00 [medline]
PHST- 2022/07/21 00:00 [pmc-release]
AID - 2101832 [pii]
AID - 10.1080/13510002.2022.2101832 [doi]
PST - ppublish
SO  - Redox Rep. 2022 Dec;27(1):158-166. doi: 10.1080/13510002.2022.2101832.