PMID- 35864383
OWN - NLM
STAT- MEDLINE
DCOM- 20220811
LR  - 20220811
IS  - 1615-7605 (Electronic)
IS  - 1615-7591 (Print)
IS  - 1615-7591 (Linking)
VI  - 45
IP  - 8
DP  - 2022 Aug
TI  - Optimisation of the fermentation media to enhance the production of the bioactive 
      isomer of vitamin menaquinone-7.
PG  - 1371-1390
LID - 10.1007/s00449-022-02752-6 [doi]
AB  - Menaquinone-7 (MK-7) offers significant health benefits; however, only the 
      all-trans form is biologically active. MK-7 produced through fermentation can 
      occur as all-trans and cis isomers, and the therapeutic value of the resulting 
      MK-7 is exclusively determined by the quantity of the all-trans isomer. 
      Therefore, this study aimed to investigate the effect of the media composition on 
      the isomer profile obtained from fermentation and determine the optimum media 
      combination to increase the concentration of the all-trans isomer and diminish 
      the production of cis MK-7. For this purpose, design of experiments (DOE) was 
      used to screen the most effective nutrients, and a central composite face-centred 
      design (CCF) was employed to optimise the media components. The optimum media 
      consisted of 1% (w/v) glucose, 2% (w/v) yeast extract, 2% (w/v) soy peptone, 2% 
      (w/v) tryptone, and 0.1% (w/v) CaCl(2). This composition resulted in an average 
      all-trans and cis isomer concentration of 36.366 mg/L and 1.225 mg/L, 
      respectively. In addition, the optimised media enabled an all-trans isomer 
      concentration 12.2-fold greater and a cis isomer concentration 2.9-fold less than 
      the unoptimised media. This study was the first to consider the development of an 
      optimised fermentation media to enhance the production of the bioactive isomer of 
      MK-7 and minimise the concentration of the inactive isomer. Furthermore, this 
      media is commercially promising, as it will improve the process productivity and 
      reduce the costs associated with the industrial fermentation of the vitamin.
CI  - (c) 2022. The Author(s).
FAU - Lal, Neha
AU  - Lal N
AD  - School of Engineering, The University of Waikato, Hamilton, 3240, New Zealand.
FAU - Seifan, Mostafa
AU  - Seifan M
AD  - School of Engineering, The University of Waikato, Hamilton, 3240, New Zealand.
FAU - Berenjian, Aydin
AU  - Berenjian A
AD  - School of Engineering, The University of Waikato, Hamilton, 3240, New Zealand. 
      aydin.berenjian@waikato.ac.nz.
AD  - Department of Agricultural and Biological Engineering, Pennsylvania State 
      University, 221 Agricultural Engineering Building, University Park, PA, 16802, 
      USA. aydin.berenjian@waikato.ac.nz.
LA  - eng
PT  - Journal Article
DEP - 20220721
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
RN  - 0 (Vitamins)
RN  - 11032-49-8 (Vitamin K 2)
RN  - 8427BML8NY (menaquinone 7)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Fermentation
MH  - *Glucose
MH  - Vitamin K 2/analogs & derivatives
MH  - *Vitamins
PMC - PMC9302956
OTO - NOTNLM
OT  - Bioactivity
OT  - Fermentation
OT  - Media optimisation
OT  - Menaquinone-7 isomers
COIS- The authors declare that they have no conflicts of interest associated with this 
      publication.
EDAT- 2022/07/22 06:00
MHDA- 2022/08/12 06:00
PMCR- 2022/07/21
CRDT- 2022/07/21 23:31
PHST- 2022/05/05 00:00 [received]
PHST- 2022/06/22 00:00 [accepted]
PHST- 2022/07/22 06:00 [pubmed]
PHST- 2022/08/12 06:00 [medline]
PHST- 2022/07/21 23:31 [entrez]
PHST- 2022/07/21 00:00 [pmc-release]
AID - 10.1007/s00449-022-02752-6 [pii]
AID - 2752 [pii]
AID - 10.1007/s00449-022-02752-6 [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2022 Aug;45(8):1371-1390. doi: 
      10.1007/s00449-022-02752-6. Epub 2022 Jul 21.