PMID- 35866032
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220723
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 10
DP  - 2022
TI  - Effect of the Human Amniotic Membrane on the Umbilical Vein Endothelial Cells of 
      Gestational Diabetic Mothers: New Insight on Inflammation and Angiogenesis.
PG  - 854845
LID - 10.3389/fbioe.2022.854845 [doi]
LID - 854845
AB  - One of the most relevant diabetes complications is impaired wound healing, mainly 
      characterized by reduced peripheral blood flow and diminished neovascularization 
      together with increased inflammation and oxidative stress. Unfortunately, 
      effective therapies are currently lacking. Recently, the amniotic membrane (AM) 
      has shown promising results in wound management. Here, the potential role of AM 
      on endothelial cells isolated from the umbilical cord vein of gestational 
      diabetes-affected women (GD-HUVECs), has been investigated. Indeed, GD-HUVECs in 
      vivo exposed to chronic hyperglycemia during pregnancy compared to control cells 
      (C-HUVECs) have shown molecular modifications of cellular homeostasis ultimately 
      impacting oxidative and nitro-oxidative stress, inflammatory phenotype, nitric 
      oxide (NO) synthesis, and bioavailability, thus representing a useful model for 
      studying the mechanisms potentially supporting the role of AM in chronic 
      non-healing wounds. In this study, the anti-inflammatory properties of AM have 
      been assessed using a monocyte-endothelium interaction assay in cells 
      pre-stimulated with tumor necrosis factor-alpha (TNF-alpha) and through vascular adhesion 
      molecule expression and membrane exposure, together with the AM impact on the 
      nuclear factor kappa-light-chain-enhancer of activated B cell (NF-kB) pathway and 
      NO bioavailability. Moreover, GD-HUVEC migration and tube formation ability were 
      evaluated in the presence of AM. The results showed that AM significantly reduced 
      TNF-alpha-stimulated monocyte-endothelium interaction and the membrane exposure of 
      the endothelial vascular and intracellular adhesion molecules (VCAM-1 and ICAM-1, 
      respectively) in both C- and GD-HUVECs. Strikingly, AM treatment significantly 
      improved vessel formation in GD-HUVECs and cell migration in both C- and 
      GD-HUVECs. These collective results suggest that AM positively affects various 
      critical pathways in inflammation and angiogenesis, thus providing further 
      validation for ongoing clinical trials in diabetic foot ulcers.
CI  - Copyright (c) 2022 Pipino, Bernabe-Garcia, Cappellacci, Stelling-Ferez, Di Tomo, 
      Santalucia, Navalon, Pandolfi and Nicolas.
FAU - Pipino, Caterina
AU  - Pipino C
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI-MeT), Department of 
      Medical, Oral and Biotechnological Sciences, University G. D'Annunzio 
      Chieti-Pescara, StemTeCh Group, Chieti, Italy.
FAU - Bernabe-Garcia, Angel
AU  - Bernabe-Garcia A
AD  - Regeneration, Molecular Oncology and TGFss, IMIB-Arrixaca, Hospital Clinico 
      Universitario Virgen de La Arrixaca, Murcia, Spain.
FAU - Cappellacci, Ilaria
AU  - Cappellacci I
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI-MeT), Department of 
      Medical, Oral and Biotechnological Sciences, University G. D'Annunzio 
      Chieti-Pescara, StemTeCh Group, Chieti, Italy.
FAU - Stelling-Ferez, Javier
AU  - Stelling-Ferez J
AD  - Regeneration, Molecular Oncology and TGFss, IMIB-Arrixaca, Hospital Clinico 
      Universitario Virgen de La Arrixaca, Murcia, Spain.
AD  - Department of Nutrition and Food Technology, UCAM, Murcia, Spain.
FAU - Di Tomo, Pamela
AU  - Di Tomo P
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI-MeT), Department of 
      Medical, Oral and Biotechnological Sciences, University G. D'Annunzio 
      Chieti-Pescara, StemTeCh Group, Chieti, Italy.
FAU - Santalucia, Manuela
AU  - Santalucia M
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI-MeT), Department of 
      Medical, Oral and Biotechnological Sciences, University G. D'Annunzio 
      Chieti-Pescara, StemTeCh Group, Chieti, Italy.
FAU - Navalon, Carlos
AU  - Navalon C
AD  - Regeneration, Molecular Oncology and TGFss, IMIB-Arrixaca, Hospital Clinico 
      Universitario Virgen de La Arrixaca, Murcia, Spain.
FAU - Pandolfi, Assunta
AU  - Pandolfi A
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI-MeT), Department of 
      Medical, Oral and Biotechnological Sciences, University G. D'Annunzio 
      Chieti-Pescara, StemTeCh Group, Chieti, Italy.
FAU - Nicolas, Francisco Jose
AU  - Nicolas FJ
AD  - Regeneration, Molecular Oncology and TGFss, IMIB-Arrixaca, Hospital Clinico 
      Universitario Virgen de La Arrixaca, Murcia, Spain.
LA  - eng
PT  - Journal Article
DEP - 20220705
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC9294233
OTO - NOTNLM
OT  - HUVECs
OT  - amniotic membrane (AM)
OT  - angiogenesis
OT  - diabetes
OT  - inflammation
OT  - wound healing
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/23 06:00
MHDA- 2022/07/23 06:01
PMCR- 2022/01/01
CRDT- 2022/07/22 02:38
PHST- 2022/01/14 00:00 [received]
PHST- 2022/05/11 00:00 [accepted]
PHST- 2022/07/22 02:38 [entrez]
PHST- 2022/07/23 06:00 [pubmed]
PHST- 2022/07/23 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 854845 [pii]
AID - 10.3389/fbioe.2022.854845 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2022 Jul 5;10:854845. doi: 10.3389/fbioe.2022.854845. 
      eCollection 2022.