PMID- 35866307
OWN - NLM
STAT- MEDLINE
DCOM- 20221011
LR  - 20221014
IS  - 1097-0347 (Electronic)
IS  - 1043-3074 (Linking)
VI  - 44
IP  - 11
DP  - 2022 Nov
TI  - Loss of exosomal miR-34c-5p in cancer-associated fibroblast for the maintenance 
      of stem-like phenotypes of laryngeal cancer cells.
PG  - 2437-2451
LID - 10.1002/hed.27155 [doi]
AB  - BACKGROUND: Cancer-associated fibroblasts (CAFs) reconstitute cancer stemness. 
      This study aims to investigate whether the loss of CAF-derived exosomal 
      miR-34c-5p contributes to the maintenance of stem-like properties of laryngeal 
      squamous cell carcinoma (LSCC). METHODS: Exosomes from primarily cultured CAFs 
      and paired normal fibroblasts (NFs) were collected and identified. The 
      differential expression of exosomal miR-34c-5p between CAFs and NFs was detected 
      by next-generation sequencing. In vitro and in vivo assays were performed to 
      examine the effects of miR-34c-5p on the maintenance of stem-like properties. 
      RESULTS: MiR-34c-5p expression is significantly reduced in CAF-derived exosomes. 
      In vitro and in vivo assays revealed that exosomal miR-34c-5p can regulate the 
      stem-like properties of LSCC cells, such as proliferation, invasion, sphere and 
      plate colony formation, chemoresistance, tumorigenicity in nude mice, as well as 
      the expression of cancer stem cell genes. CONCLUSIONS: Loss of miR-34c-5p in 
      CAF-derived exosomes contributes to the maintenance of stem-like phenotypes of 
      LSCC.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Wang, Mei
AU  - Wang M
AD  - Department of Otolaryngology, Shanghai Municipal Hospital of Traditional Chinese 
      Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
FAU - Zhuoma, Ciwang
AU  - Zhuoma C
AD  - Department of Otolaryngology, Shigatse People's Hospital, Tibet, China.
FAU - Liu, Xiaojin
AU  - Liu X
AD  - Department of Otolaryngology, Shigatse People's Hospital, Tibet, China.
FAU - Huang, Qiang
AU  - Huang Q
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Shanghai Key Clinical 
      Disciplines of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, 
      Shanghai, China.
FAU - Cai, Guoyu
AU  - Cai G
AD  - Department of Otolaryngology, Shigatse People's Hospital, Tibet, China.
FAU - Sang, Ge
AU  - Sang G
AD  - Department of Otolaryngology, Shigatse People's Hospital, Tibet, China.
FAU - Zhou, Liang
AU  - Zhou L
AUID- ORCID: 0000-0002-8588-2959
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Shanghai Key Clinical 
      Disciplines of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, 
      Shanghai, China.
FAU - Du, Huaidong
AU  - Du H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Shanghai Key Clinical 
      Disciplines of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, 
      Shanghai, China.
FAU - Wu, Chunping
AU  - Wu C
AUID- ORCID: 0000-0002-0712-8715
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Shanghai Key Clinical 
      Disciplines of Otorhinolaryngology, Eye & ENT Hospital of Fudan University, 
      Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220722
PL  - United States
TA  - Head Neck
JT  - Head & neck
JID - 8902541
RN  - 0 (MIRN34 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - *Cancer-Associated Fibroblasts/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *Laryngeal Neoplasms/pathology
MH  - Mice
MH  - Mice, Nude
MH  - *MicroRNAs/genetics/metabolism
MH  - Phenotype
OTO - NOTNLM
OT  - cancer stem cell
OT  - cancer-associated fibroblasts
OT  - exosome
OT  - laryngeal squamous cell carcinoma
OT  - miR-34c-5p
EDAT- 2022/07/23 06:00
MHDA- 2022/10/12 06:00
CRDT- 2022/07/22 04:02
PHST- 2022/06/17 00:00 [revised]
PHST- 2022/02/10 00:00 [received]
PHST- 2022/07/07 00:00 [accepted]
PHST- 2022/07/23 06:00 [pubmed]
PHST- 2022/10/12 06:00 [medline]
PHST- 2022/07/22 04:02 [entrez]
AID - 10.1002/hed.27155 [doi]
PST - ppublish
SO  - Head Neck. 2022 Nov;44(11):2437-2451. doi: 10.1002/hed.27155. Epub 2022 Jul 22.