PMID- 35866408 OWN - NLM STAT- MEDLINE DCOM- 20220725 LR - 20221028 IS - 2768-6698 (Electronic) IS - 2768-6698 (Linking) VI - 27 IP - 7 DP - 2022 Jul 14 TI - Aqueous Humor Cytokine Response in the Contralateral Eye after First-Eye Cataract Surgery in Patients with Primary Angle-Closure Glaucoma, High Myopia or Type 2 Diabetes Mellitus. PG - 222 LID - 10.31083/j.fbl2707222 [doi] AB - BACKGROUND: Bilateral sequential cataract surgery within a short period is becoming more prevalent because of the efficiency and safety of modern cataract surgery. It has been reported that the first surgical eye might affect the contralateral eye. This study investigated the cytokines involved in the immunopathogenesis of pre-existing ocular or systemic conditions, as well as the inflammatory biomarkers in response to topical stimuli, by analyzing the cytokine profile of aqueous humor (AH) from cataract patients without these morbidities as control and with type 2 diabetes mellitus (DM), primary angle-closure glaucoma (PACG) or high myopia (HM) in each eye at the beginning of first (defined as baseline) and second eye cataract surgery. METHODS: Forty patients were recruited in this cohort study (10/group). Bilateral sequential cataract surgeries were conducted at intervals of 12.08 +/- 1.2 days. Aqueous humor samples (100-200 muL/eye) were separately collected from 40 first-eyes and 40 second-eyes at the beginning of the cataract surgeries. Twenty-seven selected cytokines were detected with Luminex-multiplex immunoassay. The concentrations of cytokines in the aqueous humor and their association with pre-existing ocular or systemic conditions were analyzed and compared between and within the groups. RESULTS: Before first-eye surgery (baseline), the levels of interleukin (IL)-1ra, IL-13 and tumor necrosis factor (TNF)-alpha were significantly increased in PACG compared with controls. The levels of IL-13 were increased while that of IL-15 were decreased in HM. Compared with controls, 11 cytokines were significantly increased in DM. In the AH of the contralateral eye after first-eye cataract surgery, basic fibroblast growth factor (bFGF) was significantly more abundant in PACG and HM, while the levels of monocyte chemoattractant protein-1 (MCP-1) and interferon gamma-induced protein 10 (IP-10) were decreased in PACG. We also identified 6 significantly upregulated cytokines in DM compared with controls. Compared with baseline, there was an overlap of 5 altered cytokines in the AH of contralateral eyes after first-eye surgery between the four groups. Some were exclusively altered in each subgroup, with 1 in the control group, 4 cytokines in the PACG and HM groups, and none in the DM group. CONCLUSIONS: From the initial profile, it is observed that patients with pre-existing ocular or systemic conditions have some degree of inflammation in their eyes before surgery and in the contralateral eye after the first eye cataract surgery, which could be peculiar of the morbid conditions of the patients. Inflammation was more detectable in patients with type 2 DM before surgery. PACG and HM patients showed stronger intraocular inflammatory reactions to topical stimuli compared with controls and DM patients. Our data suggest that ophthalmologists should pay closer attention to inflammatory responses, especially in cataract patients with pre-existing conditions, although the clinical significance of these changes following surgery remains to be further investigated. CI - (c) 2022 The Author(s). Published by IMR Press. FAU - Tang, Jiancen AU - Tang J AD - Department of Ophthalmology, Shanghai East Hospital, Tongji University School of Medicine, 200120 Shanghai, China. FAU - Liu, Hanhan AU - Liu H AD - Department of Ophthalmology, Faculty of Medicine and University Hospital of Cologne, University of Cologne, 50937 Cologne, Germany. FAU - Sun, Mengsha AU - Sun M AD - Department of Ophthalmology, Shanghai East Hospital, Tongji University School of Medicine, 200120 Shanghai, China. FAU - Zhang, Xueqian AU - Zhang X AD - Department of Ophthalmology, Shanghai East Hospital, Tongji University School of Medicine, 200120 Shanghai, China. FAU - Chu, Hao AU - Chu H AD - Department of Ophthalmology, Shanghai East Hospital, Tongji University School of Medicine, 200120 Shanghai, China. FAU - Li, Qian AU - Li Q AD - Department of Ophthalmology, Shanghai East Hospital, Tongji University School of Medicine, 200120 Shanghai, China. FAU - Prokosch, Verena AU - Prokosch V AD - Department of Ophthalmology, Faculty of Medicine and University Hospital of Cologne, University of Cologne, 50937 Cologne, Germany. FAU - Cui, Hongping AU - Cui H AD - Department of Ophthalmology, Shanghai East Hospital, Tongji University School of Medicine, 200120 Shanghai, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Singapore TA - Front Biosci (Landmark Ed) JT - Frontiers in bioscience (Landmark edition) JID - 101612996 RN - 0 (Cytokines) RN - 0 (Interleukin-13) SB - IM MH - Aqueous Humor/metabolism MH - *Cataract/complications/metabolism MH - Cohort Studies MH - Cytokines/metabolism MH - *Diabetes Mellitus, Type 2/complications/metabolism/surgery MH - *Glaucoma, Angle-Closure/complications/metabolism/surgery MH - Humans MH - Inflammation/metabolism MH - Interleukin-13/metabolism MH - *Myopia/complications/metabolism/surgery OTO - NOTNLM OT - aqueous humor OT - cataract surgery OT - contralateral eye OT - cytokine OT - high myopia OT - primary angle-closure glaucoma OT - type 2 diabetes mellitus COIS- The authors declare no conflict of interest. EDAT- 2022/07/23 06:00 MHDA- 2022/07/26 06:00 CRDT- 2022/07/22 04:58 PHST- 2022/05/16 00:00 [received] PHST- 2022/06/07 00:00 [revised] PHST- 2022/07/06 00:00 [accepted] PHST- 2022/07/22 04:58 [entrez] PHST- 2022/07/23 06:00 [pubmed] PHST- 2022/07/26 06:00 [medline] AID - S2768-6701(22)00584-6 [pii] AID - 10.31083/j.fbl2707222 [doi] PST - ppublish SO - Front Biosci (Landmark Ed). 2022 Jul 14;27(7):222. doi: 10.31083/j.fbl2707222.