PMID- 35867944
OWN - NLM
STAT- MEDLINE
DCOM- 20221018
LR  - 20221018
IS  - 1473-5733 (Electronic)
IS  - 0957-5235 (Linking)
VI  - 33
IP  - 7
DP  - 2022 Oct 1
TI  - Harmonizing factor assay-related testing performed in a large laboratory network.
PG  - 402-411
LID - 10.1097/MBC.0000000000001155 [doi]
AB  - Coagulation factor testing is commonly performed within haemostasis laboratories, 
      either to assess for bleeding disorders, such as haemophilia, or to investigate 
      unexplained prolongation in routine coagulation assays. The aim of this 
      evaluation was to harmonize procedures and normal reference ranges (NRRs) for 
      investigation of coagulation factors on the ACL TOP 50 family of instruments in a 
      large laboratory network. We employed comparative evaluations using newly 
      installed ACL TOPs 550 and 750 and HemosIL reagents vs. existing 'reference' 
      instrumentation and reagents, predominantly Stago and Siemens, as well as 
      assessment of factor sensitivity in routine coagulation assays, prothrombin time 
      (PT) and activated partial thromboplastin time (APTT). Also, establishment of 
      coagulation factor NRRs using normal plasma samples. HemosIL factor assays showed 
      good comparability with the existing reference methods ( R > 0.9). Factor 
      sensitivity for PT and APTT assays were acceptable at around 30 U/dl. NRRs were 
      established and harmonized across the laboratory network. This evaluation of 
      factor testing on ACL TOP 50 Family instruments identified overall acceptable 
      performance using Werfen reagents and enabled harmonization of coagulation factor 
      testing in our large network.
CI  - Copyright (c) 2022 Wolters Kluwer Health, Inc. All rights reserved.
FAU - Favaloro, Emmanuel J
AU  - Favaloro EJ
AD  - Haematology, Institute of Clinical Pathology and Medical Research (ICPMR), NSW 
      Health Pathology, Westmead Hospital.
AD  - Sydney Centres for Thrombosis and Haemostasis, Westmead.
AD  - Faculty of Science and Health, Charles Sturt University, Wagga Wagga.
FAU - Mohammed, Soma
AU  - Mohammed S
AD  - Haematology, Institute of Clinical Pathology and Medical Research (ICPMR), NSW 
      Health Pathology, Westmead Hospital.
FAU - Vong, Ronny
AU  - Vong R
AD  - Haematology, Institute of Clinical Pathology and Medical Research (ICPMR), NSW 
      Health Pathology, Westmead Hospital.
FAU - Chapman, Kent
AU  - Chapman K
AD  - Haematology, NSW Health Pathology, John Hunter Hospital, Newcastle.
FAU - Swanepoel, Priscilla
AU  - Swanepoel P
AD  - Haematology, NSW Health Pathology, John Hunter Hospital, Newcastle.
FAU - Kershaw, Geoffrey
AU  - Kershaw G
AD  - Haematology, NSW Health Pathology, Prince Alfred Hospital, Camperdown.
FAU - Cai, Nancy
AU  - Cai N
AD  - Haematology, NSW Health Pathology, Prince Alfred Hospital, Camperdown.
FAU - Just, Sarah
AU  - Just S
AD  - Haematology, NSW Health Pathology, Royal North Shore Hospital, St Leonards.
FAU - Connelly, Lynne
AU  - Connelly L
AD  - Haematology, NSW Health Pathology, Royal North Shore Hospital, St Leonards.
FAU - Brighton, Timothy
AU  - Brighton T
AD  - Haematology, NSW Health Pathology, Prince of Wales Hospital, Randwick.
FAU - Pasalic, Leonardo
AU  - Pasalic L
AD  - Haematology, Institute of Clinical Pathology and Medical Research (ICPMR), NSW 
      Health Pathology, Westmead Hospital.
AD  - Sydney Centres for Thrombosis and Haemostasis, Westmead.
AD  - Westmead Clinical School, University of Sydney, Westmead, New South Wales, 
      Australia.
LA  - eng
PT  - Journal Article
DEP - 20220722
PL  - England
TA  - Blood Coagul Fibrinolysis
JT  - Blood coagulation & fibrinolysis : an international journal in haemostasis and 
      thrombosis
JID - 9102551
RN  - 0 (Blood Coagulation Factors)
SB  - IM
MH  - *Blood Coagulation Factors
MH  - Blood Coagulation Tests/methods
MH  - Humans
MH  - *Laboratories
MH  - Partial Thromboplastin Time
MH  - Prothrombin Time/methods
EDAT- 2022/07/23 06:00
MHDA- 2022/10/19 06:00
CRDT- 2022/07/22 15:53
PHST- 2022/07/23 06:00 [pubmed]
PHST- 2022/10/19 06:00 [medline]
PHST- 2022/07/22 15:53 [entrez]
AID - 00001721-202210000-00006 [pii]
AID - 10.1097/MBC.0000000000001155 [doi]
PST - ppublish
SO  - Blood Coagul Fibrinolysis. 2022 Oct 1;33(7):402-411. doi: 
      10.1097/MBC.0000000000001155. Epub 2022 Jul 22.