PMID- 35869735
OWN - NLM
STAT- MEDLINE
DCOM- 20220726
LR  - 20220729
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 68
IP  - 2
DP  - 2022 Feb 28
TI  - Influences of ALDH2 on Cardiomyocyte Apoptosis in Heart Failure Rats Through 
      Regulating PINK1-Parkin Signaling Pathway-Mediated Mitophagy.
PG  - 94-102
LID - 10.14715/cmb/2022.68.2.14 [doi]
AB  - The study aimed to investigate the influences of aldehyde dehydrogenase 2 (ALDH2) 
      on cardiomyocyte apoptosis in heart failure (HF) rats through regulating the PTEN 
      induced putative kinase 1 (PINK1)-Parkin signaling pathway-mediated mitophagy. 
      The rat model of HF was established, and the rats were randomly divided into 
      model group (HF model, n=20) and ALDH2 group (intervention with ALDH2, n=20), 
      with a normal group (n=20) set. After successful modeling, MRI and ECG were 
      applied to detect the cardiac function indexes of the rats. The myocardial 
      function index creatine kinase (CK) was measured, the status of myocardial tissue 
      injury was determined using hematoxylin and eosin staining, and the apoptosis was 
      observed via terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) staining. The activity of ALDH2 was detected, and the expression 
      levels of genes and proteins were measured through quantitative polymerase chain 
      reaction (qPCR) and Western blotting assay. The model group had notably decreased 
      fractional shortening (FS) and ejection fraction (EF) and remarkably increased 
      left ventricular end-diastolic diameter (LVEDD) and left ventricular end-systolic 
      diameter (LVESD) compared with the normal group (p<0.05). The activity of ALDH2 
      declined obviously in the model group. The myocardial tissue injury was severer 
      in the model group, and the number of apoptotic cells in myocardial tissues was 
      greater in the model group than that in other groups (p<0.05). The model group 
      manifested higher expression levels of Caspase-3 and light chain 3 (LC3) than the 
      ALDH2 group (p<0.05) but significantly lower expression levels of PINK1, Parkin 
      and B-cell lymphoma-2 (Bcl-2) (p<0.05). In comparison with those in the model 
      group, the protein expression levels of PINK1, Parkin and Bcl-2 in myocardial 
      tissues were prominently higher in the ALDH2 group (p<0.05). ALDH2 can inhibit 
      cardiomyocyte apoptosis in HF rats by activating the PINK1-Parkin signaling 
      pathway-mediated mitophagy, which is conducive to the recovery of HF.
FAU - Abudureyimu, Miyesaier
AU  - Abudureyimu M
AD  - Shanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, Fudan University. 
      fuchuitu8662868@163.com.
FAU - Zhao, Lifang
AU  - Zhao L
AD  - Shanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, Fudan University. 
      fuchuitu8662868@163.com.
FAU - Luo, Xuanming
AU  - Luo X
AD  - Shanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, Fudan University. 
      fuchuitu8662868@163.com.
FAU - Wang, Xiang
AU  - Wang X
AD  - Shanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, Fudan University. 
      fuchuitu8662868@163.com.
FAU - Liu, Haibo
AU  - Liu H
AD  - Qingpu Branch of Zhongshan Hospital, Fudan University. fuchuitu8662868@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220228
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - EC 1.2.1.3 (Aldehyde Dehydrogenase, Mitochondrial)
RN  - EC 1.2.1.3 (Aldh2 protein, rat)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.- (Protein Kinases)
SB  - IM
MH  - Aldehyde Dehydrogenase, Mitochondrial/genetics/metabolism
MH  - Animals
MH  - Apoptosis/genetics
MH  - Disease Models, Animal
MH  - *Heart Failure/metabolism
MH  - Mitophagy
MH  - *Myocytes, Cardiac/metabolism
MH  - Protein Kinases/genetics/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - Ubiquitin-Protein Ligases/genetics/metabolism
EDAT- 2022/07/24 06:00
MHDA- 2022/07/27 06:00
CRDT- 2022/07/23 03:34
PHST- 2022/04/01 00:00 [received]
PHST- 2022/07/23 03:34 [entrez]
PHST- 2022/07/24 06:00 [pubmed]
PHST- 2022/07/27 06:00 [medline]
AID - 10.14715/cmb/2022.68.2.14 [doi]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2022 Feb 28;68(2):94-102. doi: 
      10.14715/cmb/2022.68.2.14.